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Abstract

A novel actinomycete, designated strain NEAU-Ht49, was isolated from sea sand sampled in Sanya and characterized by using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-Ht49 was most closely related to SDA37 (98.8 %), NEAU-G17 (98.6 %), ATCC 43269 (98.6 %) and NEAU-Gz5 (98.6 %). The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain NEAU-Ht49 formed a cluster with SDA37, NEAU-G17, ATCC 43269, NEAU-Gz5 and CS5-AC15 (96.4 %). Meso-diaminopimelic acid was detected in its cell walls and glucose, madurose, mannose and ribose were detected in whole-cell hydrolysate. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositolmannoside and two unidentified lipids. The majoy menaquinone was MK-10(H) and the minor menaquinones were MK-9(H) and MK-9(H). The major fatty acids were C, Cω9, 10-methyl C and iso-C. Moreover, morphological and chemotaxonomic characteristics of properties of strain NEAU-Ht49 also confirmed the affiliation of the isolate to the genus . However, DNA–DNA relatedness, physiological and biochemical data showed that strain NEAU-Ht49 could be distinguished from its closest relatives. Therefore, strain NEAU-Ht49 represents a novel species of the genus , for which the name sp. nov. is proposed, with strain NEAU-Ht49 (=CGMCC 4.7499=JCM 32659) as the type strain.

Funding
This study was supported by the:
  • Xiangjing Wang , the National Natural Science Foundation of China , (Award 31572070)
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2019-10-31
2021-01-23
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