RT Journal Article SR Electronic(1) A1 Hu, Jiangmeihui A1 Han, Chuanyu A1 Yu, Bing A1 Zhao, Junwei A1 Guo, Xiaowei A1 Shen, Yue A1 Wang, Xiangjing A1 Xiang, WenshengYR 2020 T1 Actinomadura harenae sp. nov., a novel actinomycete isolated from sea sand in Sanya JF International Journal of Systematic and Evolutionary Microbiology, VO 70 IS 2 SP 766 OP 772 DO https://doi.org/10.1099/ijsem.0.003819 PB Microbiology Society, SN 1466-5034, AB A novel actinomycete, designated strain NEAU-Ht49T, was isolated from sea sand sampled in Sanya and characterized by using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-Ht49T was most closely related to Actinomadura rhizosphaerae SDA37T (98.8 %), Actinomadura logoneensis NEAU-G17T (98.6 %), Actinomadura oligospora ATCC 43269T (98.6 %) and Actinomadura gamaensis NEAU-Gz5T (98.6 %). The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain NEAU-Ht49T formed a cluster with A. rhizosphaerae SDA37T, A. logoneensis NEAU-G17T, A. oligospora ATCC 43269T, A. gamaensis NEAU-Gz5T and Actinomadura rupiterrae CS5-AC15T (96.4 %). Meso-diaminopimelic acid was detected in its cell walls and glucose, madurose, mannose and ribose were detected in whole-cell hydrolysate. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositolmannoside and two unidentified lipids. The majoy menaquinone was MK-10(H6) and the minor menaquinones were MK-9(H4) and MK-9(H8). The major fatty acids were C16 : 0, C18 : 1ω9c, 10-methyl C18 : 0 and iso-C16 : 0. Moreover, morphological and chemotaxonomic characteristics of properties of strain NEAU-Ht49T also confirmed the affiliation of the isolate to the genus Actinomadura . However, DNA–DNA relatedness, physiological and biochemical data showed that strain NEAU-Ht49T could be distinguished from its closest relatives. Therefore, strain NEAU-Ht49T represents a novel species of the genus Actinomadura , for which the name Actinomadura harenae sp. nov. is proposed, with strain NEAU-Ht49T (=CGMCC 4.7499T=JCM 32659T) as the type strain., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003819