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Volume 69, Issue 10

sp. nov., a new actinobacterium isolated from a cave Free

Abstract

A novel Gram-stain-positive actinobacterial strain, designated C9-28, was isolated from soil sampled in a natural cave on Jeju Island, Republic of Korea. Strain C9-28 morphologically exhibited a rod–coccus life cycle and grew at 10–37 °C (optimum, 30 °C), pH 6–9 (optimum, pH 7) and 0–3 % (optimum, absence of NaCl). In the maximum-likelihood tree based on 16S rRNA gene sequences, strain C9-28 formed a sublineage between a clade and the type strain of . The closest relatives of strain C9-28 were the type strains of (98.88 % 16S rRNA gene sequence similarity), (98.88 %) and (98.60 %). The phylogenomic tree based on whole genome sequences supported the distinct position of the novel strain within the genus . The following chemotaxonomic characteristics also supported the assignment to the genus: -diaminopimelic acid; arabinose and galactose in whole-cell hydrolysates; the predominant menaquinone of MK-8(H); and polar lipids including diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, three unidentified glycolipids and two unidentified lipids. The predominant cellular fatty acids were C, summed feature 3 (Cω7 and/or Cω6), Cω9 and C. Based on the values of average nucleotide identity and digital DNA–DNA hybridization from whole genome sequences, and DNA–DNA hybridization between the isolate and the closest relatives, strain C9-28 (=KACC 19823=DSM 107559) represents a novel species of the genus , for which the name sp. nov. is proposed.

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Keyword(s): 16S rRNA gene , cave , genome , polyphasic taxonomic approach and Rhodococcus subtropicus
© 2019 IUMS
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2019-10-01
2024-04-26
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Rhodococcus subtropicus sp. nov., a new actinobacterium isolated from a cave
Int J Syst Evol Microbiol 69, 3128 (2019); https://doi.org/10.1099/ijsem.0.003601
/content/journal/ijsem/10.1099/ijsem.0.003601
/content/journal/ijsem/10.1099/ijsem.0.003601
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