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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 68, Issue 9

Actinomadura deserti sp. nov., isolated from desert soil Free

Abstract

A Gram-positive, strictly aerobic actinobacterium, designated BMP B8004, was isolated from desert soil collected in Xinjiang Province, Northwest China. It produced an extensively branched non-fragmenting substrate mycelium, and very scanty aerial mycelium that formed a short hooked chain of arthrospores with a smooth surface. Optimum growth occurred at 28 °C, pH 7.0 and 0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BMP B8004 formed a distinct phyletic lineage within the genus Actinomadura . It shared the highest 16S rRNA gene sequence similarity to Actinomadura apis IM17-1 (99.2 %) and Actinomadura rifamycini NBRC 14183 (98.6 %). However, it could be distinguished from the two closest strains based on the low levels of DNA–DNA relatedness (52.7±0.7 and 45±1.8 %, respectively). Chemotaxonomic characteristics, including the main phospholipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides, the major menaquinones MK-9(H6) and MK-9(H8), the predominant fatty acids iso-C16 : 0, C16 : 0, C18 : 0 10-methyl and C18 : 1ω9c, were also consistent with the properties of the genus Actinomadura . The DNA G+C content of strain BMP B8004 was 71.9 mol%. Based on phenotypic and genotypic features, strain BMP B8004 is considered to represent a novel species of the genus Actinomadura , for which the name Actinomadura deserti sp. nov. is proposed. The type strain is BMP B8004 (=CGMCC 4.7432=KCTC 39998).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , polyphasic taxonomy , sandy soil and Thermomonosporaceae
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2018-08-09
2025-04-27
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Actinomadura deserti sp. nov., isolated from desert soil
Int J Syst Evol Microbiol 68, 2930 (2018); https://doi.org/10.1099/ijsem.0.002922
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