RT Journal Article SR Electronic(1) A1 Cao, Chengliang A1 Xu, Tangyu A1 Liu, Jinjuan A1 Cai, Xiaorui A1 Sun, Yong A1 Qin, Sheng A1 Jiang, Jihong A1 Huang, YingYR 2018 T1 Actinomadura deserti sp. nov., isolated from desert soil JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 9 SP 2930 OP 2935 DO https://doi.org/10.1099/ijsem.0.002922 PB Microbiology Society, SN 1466-5034, AB A Gram-positive, strictly aerobic actinobacterium, designated BMP B8004T, was isolated from desert soil collected in Xinjiang Province, Northwest China. It produced an extensively branched non-fragmenting substrate mycelium, and very scanty aerial mycelium that formed a short hooked chain of arthrospores with a smooth surface. Optimum growth occurred at 28 °C, pH 7.0 and 0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BMP B8004T formed a distinct phyletic lineage within the genus Actinomadura . It shared the highest 16S rRNA gene sequence similarity to Actinomadura apis IM17-1T (99.2 %) and Actinomadura rifamycini NBRC 14183T (98.6 %). However, it could be distinguished from the two closest strains based on the low levels of DNA–DNA relatedness (52.7±0.7 and 45±1.8 %, respectively). Chemotaxonomic characteristics, including the main phospholipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides, the major menaquinones MK-9(H6) and MK-9(H8), the predominant fatty acids iso-C16 : 0, C16 : 0, C18 : 0 10-methyl and C18 : 1ω9c, were also consistent with the properties of the genus Actinomadura . The DNA G+C content of strain BMP B8004T was 71.9 mol%. Based on phenotypic and genotypic features, strain BMP B8004T is considered to represent a novel species of the genus Actinomadura , for which the name Actinomadura deserti sp. nov. is proposed. The type strain is BMP B8004T (=CGMCC 4.7432T=KCTC 39998T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002922