1887

Abstract

A novel orange-pigmented, Gram-stain-negative and strictly aerobic bacterium, designated strain HR-U, was isolated from a water sample of the Han River located in the Republic of Korea. Cells were catalase-positive and oxidase-positive, and non-motile curved rods without flagella. The strain grew at 5–35 °C (optimum 25 °C), pH 6–10 (optimum of 7–8) and 0–3 % (w/v) NaCl (optimum 0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HR-U formed a tight phylogenic lineage with DSM 21668 and ‘’ 63MJ-2. The 16S rRNA gene sequence comparison of strain HR-U and other reported type strains showed that it shared the highest sequence similarity to DSM 21668 (96.9 %) and ‘’ 63MJ-2 (96.6 %), and had lower similarities than 87.0 % to other bacteria with validly published names. Average nucleotide identity and DNA–DNA hybridization values between train HR-U and the most closely related strain were 71.7 and 18.4 %, respectively. The major respiratory quinone was menaquinone-7, and the dominant fatty acids (>5 %) consisted of C 5, summed feature 3 (comprising Cω7 and/or iso-C 2-OH) and iso-C. The polar lipids comprised phosphatidylethanolamine, four unidentified aminolipids and three unidentified lipids. The DNA G+C content was 47.9 mol%. Based on the genotypic, chemotaxonomic and phenotypic analyses, strain HR-U represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HR-U (=KACC 19409=JCM 32267).

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2018-06-01
2020-03-29
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