1887

Abstract

A Gram-stain-negative and non-motile bacterial strain that formed rods and orange-pigmented colonies, designated HMF6543, was isolated from sand of seashore on the South Sea, Republic of Korea. Strain HMF6543 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HMF6543 belonged to the genus Sabulilitoribacter . The most closest related species was Sabulilitoribacter multivorans M-M16 (96.7 %, sequence similarity). Strain HMF6543 contained MK-6 as the predominant menaquinone and iso-C15 : 1 G, iso-C15 : 0 and iso-C17 : 0 3-OH as the major fatty acids. The major polar lipids detected in strain HMF6543 were phosphatidylethanolamine, two unidentified aminolipids, one unidentified glycolipid and three unidentified lipids. The DNA G+C content of strain HMF6543 was 31.9 mol%. On the basis of the evidence presented in this study, strain HMF6543 represents a novel species of the genus Sabulilitoribacter , for which the name Sabulilitoribacter arenilitoris sp. nov. is proposed. The type strain is HMF6543 (=KCTC 52401=NBRC 112674).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002378
2017-10-06
2019-12-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/11/4796.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002378&mimeType=html&fmt=ahah

References

  1. Jooste PJ. The taxonomy and significance of Flavobacterium-Cytophaga strains from dairy sources. PhD thesis, University of the Orange Free State, South Africa 1985
  2. Bernardet JF. Family I. Flavobacteriaceae Reichenbach 1992. In Krieg NR, Staley JT, Brown DR, Hedlund BP, Paster BJ et al. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed.vol.4 New York: Springer; 2011; pp. 106– 111
    [Google Scholar]
  3. Zhang XY, Liu A, Liu C, Li H, Li GW et al. Arenitalea lutea gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from intertidal sand. Int J Syst Evol Microbiol 2013; 63: 2853– 2858 [CrossRef] [PubMed]
    [Google Scholar]
  4. Yoon BJ, Lee DH, Kang BJ, Kahng HY, Oh YS et al. Hyunsoonleella jejuensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seawater. Int J Syst Evol Microbiol 2010; 60: 382– 386 [CrossRef] [PubMed]
    [Google Scholar]
  5. Park S, Jung YT, Lee JS, Lee KC, Yoon JH. Sabulilitoribacter multivorans gen. nov., sp. nov., a polysaccharide-degrading bacterium of the family Flavobacteriaceae isolated from seashore sand. Antonie van Leeuwenhoek 2013; 104: 973– 981 [CrossRef] [PubMed]
    [Google Scholar]
  6. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991; pp. 125– 175
    [Google Scholar]
  7. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67: 1613– 1617 [CrossRef] [PubMed]
    [Google Scholar]
  8. Jeon YS, Lee K, Park SC, Kim BS, Cho YJ et al. EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. Int J Syst Evol Microbiol 2014; 64: 689– 691 [CrossRef] [PubMed]
    [Google Scholar]
  9. Pruesse E, Peplies J, Glöckner FO. SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 2012; 28: 1823– 1829 [CrossRef] [PubMed]
    [Google Scholar]
  10. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33: 1870– 1874 [CrossRef] [PubMed]
    [Google Scholar]
  11. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4: 406– 425 [PubMed]
    [Google Scholar]
  12. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17: 368– 376 [CrossRef] [PubMed]
    [Google Scholar]
  13. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20: 406– 416 [CrossRef]
    [Google Scholar]
  14. Jukes TH, Cantor CR. Evolution of protein molecules. In Munro HN. (editor) Mammalian Protein Metabolism, 3rd ed. New York: Academic Press; 1969; pp. 21– 132 [Crossref]
    [Google Scholar]
  15. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16: 111– 120 [CrossRef] [PubMed]
    [Google Scholar]
  16. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39: 783– 791 [CrossRef] [PubMed]
    [Google Scholar]
  17. Brown AE. Benson’s Microbiological Application Laboratory Manual in General Microbiology, 10th ed. New York: McGraw-Hill; 2007
    [Google Scholar]
  18. Hucker GJ. A new modification and application of the Gram stain. J Bacteriol 1921; 6: 395– 397 [PubMed]
    [Google Scholar]
  19. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on systematics of prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52: 1049– 1070 [CrossRef] [PubMed]
    [Google Scholar]
  20. CLSI Performance Standards for Antimicrobial Disk Susceptibility Testing: Approved Standard, 11th ed. CLSI Document M02-A11 PA: Clinical and Laboratory Standards Institute; 2012
    [Google Scholar]
  21. Gonzalez JM, Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 2002; 4: 770– 773 [CrossRef] [PubMed]
    [Google Scholar]
  22. Joung Y, Lee BI, Kang H, Kim H, Joh K. Chitinimonas viridis sp. nov., isolated from a mesotrophic artificial lake. Int J Syst Evol Microbiol 2014; 64: 1123– 1126 [CrossRef] [PubMed]
    [Google Scholar]
  23. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  24. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2: 233– 241 [CrossRef]
    [Google Scholar]
  25. Collins MD. Analysis of isoprenoid quinones. In Gottschalk G. (editor) Methods in Microbiologyvol.18 New York: Academic Press; 1985; pp. 329– 366
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002378
Loading
/content/journal/ijsem/10.1099/ijsem.0.002378
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error