1887

Abstract

A novel Gram-stain-negative, yellow-pigmented, catalase- and oxidase-positive, non-endospore-forming, flagellated bacterium, designated strain Yeonmyeong 2-22, was isolated from surface seawater of Geoje Island, Republic of Korea. Strain Yeonmyeong 2-22 showed algalytic activity against the seven strains tested: Cochlodinium polykrikoides, Chattonella marina, Heterosigma akashiwo, Scrippsiella trochoidea, Heterocapsa triquetra, Prorocentrum minimum and Skeletonema costatum. A taxonomic study was carried out based on a polyphasic approach to characterize the exact taxonomic position of strain Yeonmyeong 2-22. The bacterium was able to grow at 10–40 °C, at salinities from 0 to 9 %, at pH from 4.0 to 9.0 and was not able to degrade gelatin or casein. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain Yeonmyeong 2-22 was considered to represent a novel species of the genus Porphyrobacter , which belongs to the family Erythrobacteraceae, and was related most closely to Porphyrobacter dokdonensis DSW-74 with 97.23 % 16S rRNA gene sequence similarity. The dominant cellular fatty acids of strain Yeonmyeong 2-22 were C18 : 1ω7c (49.7 %), C16 : 0 (12.0 %) and 11-methyl C18 : 1ω7c (11.5 %), and ubiquinone-10 (Q-10) was the predominant respiratory lipoquinone. The genomic DNA G+C content of strain Yeonmyeong 2-22 was calculated to be 63.0 mol%. Phenotypic characteristics of the novel strain also differed from other members of the genus Porphyrobacter . On the basis of polyphasic taxonomic data, strain Yeonmyeong 2-22represents as a novel species of the genus Porphyrobacter , for which the name of Porphyrobacter algicida sp. nov. is proposed. The type strain is Yeonmyeong 2-22 (=KEMB 9005-328=JCM 31499).

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2017-10-06
2019-10-23
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References

  1. Fuerst JA, Hawkins JA, Holmes A, Sly LI, Moore CJ et al. Porphyrobacter neustonensis gen. nov., sp. nov., an aerobic bacteriochlorophyll-synthesizing budding bacterium from fresh water. Int J Syst Bacteriol 1993; 43: 125– 134 [CrossRef] [PubMed]
    [Google Scholar]
  2. Hanada S, Kawase Y, Hiraishi A, Takaichi S, Matsuura K et al. Porphyrobacter tepidarius sp. nov., a moderately thermophilic aerobic photosynthetic bacterium isolated from a hot spring. Int J Syst Bacteriol 1997; 47: 408– 413 [CrossRef] [PubMed]
    [Google Scholar]
  3. Hiraishi A, Yonemitsu Y, Matsushita M, Shin YK, Kuraishi H et al. Characterization of Porphyrobacter sanguineus sp. nov., an aerobic bacteriochlorophyll-containing bacterium capable of degrading biphenyl and dibenzofuran. Arch Microbiol 2002; 178: 45– 52 [CrossRef] [PubMed]
    [Google Scholar]
  4. Rainey FA, Silva J, Nobre MF, Silva MT, da Costa MS. Porphyrobacter cryptus sp. nov., a novel slightly thermophilic, aerobic, bacteriochlorophyll a-containing species. Int J Syst Evol Microbiol 2003; 53: 35– 41 [CrossRef] [PubMed]
    [Google Scholar]
  5. Yoon JH, Lee MH, Oh TK. Porphyrobacter donghaensis sp. nov., isolated from sea water of the East Sea in Korea. Int J Syst Evol Microbiol 2004; 54: 2231– 2235 [CrossRef] [PubMed]
    [Google Scholar]
  6. Yoon JH, Kang SJ, Lee MH, Oh HW, Oh TK et al. Porphyrobacter dokdonensis sp. nov., isolated from sea water. Int J Syst Evol Microbiol 2006; 56: 1079– 1083 [CrossRef] [PubMed]
    [Google Scholar]
  7. Furuhata K, Edagawa A, Miyamoto H, Kawakami Y, Fukuyama M. Porphyrobacter colymbi sp. nov. isolated from swimming pool water in Tokyo, Japan. J Gen Appl Microbiol 2013; 59: 245– 250 [CrossRef]
    [Google Scholar]
  8. Coil DA, Flanagan JC, Stump A, Alexiev A, Lang JM et al. Porphyrobacter mercurialis sp. nov., isolated from a stadium seat and emended description of the genus Porphyrobacter. PeerJ 2015; 3: e1400 [CrossRef] [PubMed]
    [Google Scholar]
  9. Anderson DM, Glibert PM, Burkholder JM. Harmful algal blooms and eutrophication: nutrient sources, composition, and consequences. Estuaries 2002; 25: 704– 726 [CrossRef]
    [Google Scholar]
  10. Hallegraeff GM. A review of harmful algal blooms and their apparent global increase. Phycologia 1993; 32: 79– 99 [CrossRef]
    [Google Scholar]
  11. Mayali X, Azam F. Algicidal bacteria in the sea and their impact on algal blooms. J Eukaryot Microbiol 2004; 51: 139– 144 [CrossRef] [PubMed]
    [Google Scholar]
  12. Seong KA, Jeong HJ. Interactions between marine bacteria and red tide organisms in Korean waters. Algae 2013; 28: 297– 305 [CrossRef]
    [Google Scholar]
  13. Kristyanto S. Studies on biological control of harmful red tides by a novel bio-ceramic biofilm system Thesis Suwon, Rep Korea: Kyonggi University; 2016
    [Google Scholar]
  14. Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 1987; 19: 11– 15
    [Google Scholar]
  15. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173: 697– 703 [CrossRef]
    [Google Scholar]
  16. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67: 1613– 1617 [CrossRef] [PubMed]
    [Google Scholar]
  17. Wu HX, Lai PY, Lee OO, Zhou XJ, Miao L et al. Erythrobacter pelagi sp. nov., a member of the family Erythrobacteraceae isolated from the Red Sea. Int J Syst Evol Microbiol 2012; 62: 1348– 1353 [CrossRef] [PubMed]
    [Google Scholar]
  18. Yoon JH, Oh TK, Park YH. Erythrobacter seohaensis sp. nov. and Erythrobacter gaetbuli sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 2005; 55: 71– 75 [CrossRef] [PubMed]
    [Google Scholar]
  19. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30: 2725– 2729 [CrossRef] [PubMed]
    [Google Scholar]
  20. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25: 4876– 4882 [CrossRef] [PubMed]
    [Google Scholar]
  21. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999; 41: 95– 98
    [Google Scholar]
  22. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4: 406– 425 [PubMed]
    [Google Scholar]
  23. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17: 368– 376 [CrossRef] [PubMed]
    [Google Scholar]
  24. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20: 406– 416 [CrossRef]
    [Google Scholar]
  25. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16: 111– 120 [CrossRef] [PubMed]
    [Google Scholar]
  26. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39: 783– 791 [CrossRef] [PubMed]
    [Google Scholar]
  27. Beveridge TJ, Lawrence JR, Murray RGE. Sampling and staining for light microscopy. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM. et al. (editors) In Methods for General and Molecular Microbiology Washington, DC: American Society for Microbiology; 2007; pp. 19– 33
    [Google Scholar]
  28. Lanyi B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987; 19: 1– 67
    [Google Scholar]
  29. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) In Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp. 607– 654
    [Google Scholar]
  30. MacFaddin JF. Biochemical Tests for Identification of Medical Bacteria, 2nd ed. Baltimore, MD: Williams & Wilkins; 1980
    [Google Scholar]
  31. Ruddell KA, Anselmo CR. Antibiotic susceptibility testing of Gram-negative nonfermentative bacilli. Antimicrob Agents Chemother 1975; 7: 400– 412 [CrossRef] [PubMed]
    [Google Scholar]
  32. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  33. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2: 233– 241 [CrossRef]
    [Google Scholar]
  34. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37: 463– 464 [Crossref]
    [Google Scholar]
  35. Komagata K, Suzuki K. Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19: 161– 207 [Crossref]
    [Google Scholar]
  36. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984; 25: 125– 128 [CrossRef]
    [Google Scholar]
  37. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39: 159– 167 [CrossRef]
    [Google Scholar]
  38. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39: 224– 229 [CrossRef]
    [Google Scholar]
  39. Stackebrandt E, Goebel BM. Taxonomic note: A place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 1994; 44: 846– 849 [CrossRef]
    [Google Scholar]
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