1887

Abstract

Nine anamorphic yeast strains isolated from various plant leaves collected in southern China were phylogenetically characterized based on sequences of the internal transcribed spacer (ITS) region, the D1/D2 domains of the large subunit (LSU) rRNA gene, the small subunit (SSU) rRNA gene, the two subunits of the RNA polymerase II gene (RPB1 and RPB2) and the translation elongation factor 1-α (TEF1). Phylogenetic analysis of the combined sequences of the six genes showed that the new strains formed a distinct clade in the class Microbotryomycetes but could not be assigned to any of the existing genera, families or orders of the class. Three separate groups were consistently resolved from the nine new strains based on the combined sequences of the six genes and single gene sequences of ITS, RPB1, RPB2 and TEF1. The results suggest that the nine yeast strains compared represent three novel species in a novel genus. The names Heitmania gen. nov. (MycoBank registration number MB819987), Heitmania litseae sp. nov. (MB820112, type strain CGMCC 2.5697=CBS 14756), Heitmania castanopsis sp. nov. (MB819988, CGMCC 2.5698=CBS 14750) and Heitmania elacocarpi sp. nov. (MB820113, CGMCC 2.5695=CBS 14752) are proposed for the new taxa.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002323
2017-09-21
2019-12-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/11/4534.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002323&mimeType=html&fmt=ahah

References

  1. Bauer R, Begerow D, Sampaio JP, Weiβ M, Oberwinkler F. The simple-septate basidiomycetes: a synopsis. Mycol Prog 2006; 5: 41– 66 [CrossRef]
    [Google Scholar]
  2. Aime MC, Matheny PB, Henk DA, Frieders EM, Nilsson RH et al. An overview of the higher level classification of Pucciniomycotina based on combined analyses of nuclear large and small subunit rDNA sequences. Mycologia 2006; 98: 896– 905 [CrossRef] [PubMed]
    [Google Scholar]
  3. Aime MC, Toome M, McLaughlin DJ. Pucciniomycotina. In McLaughlin DJ, Spatafora JW. (editors) The Mycota, Vol. VII, Part A: Systematics and Evolution, 2nd ed. Berlin: Springer-Verlag; 2014; pp. 271– 294
    [Google Scholar]
  4. Wang QM, Groenewald M, Takashima M, Theelen B, Han PJ et al. Phylogeny of yeasts and related filamentous fungi within Pucciniomycotina determined from multigene sequence analyses. Stud Mycol 2015; 81: 27– 53 [CrossRef] [PubMed]
    [Google Scholar]
  5. Bauer R, Oberwinkler F. The colacosomes: new structures at the host-parasite interface of a mycoparasitic basidiomycete. Botanica Acta 1991; 104: 53– 57 [CrossRef]
    [Google Scholar]
  6. Sampaio JP, Gadanho M, Bauer R, Weiß M. Taxonomic studies in the Microbotryomycetidae: Leucosporidium golubevii sp. nov., Leucosporidiella gen. nov. and the new orders Leucosporidiales and Sporidiobolales. Mycol Prog 2003; 2: 53– 68 [CrossRef]
    [Google Scholar]
  7. Bauer R, Oberwinkler F. Cellular basidiomycete-fungus interactions. In Varma A, Abbott L, Werner D, Hampp R. (editors) Plant Surface Microbiology Berlin Heidelberg: Springer; 2008; pp. 267– 279 [Crossref]
    [Google Scholar]
  8. Wang QM, Yurkov AM, Göker M, Lumbsch HT, Leavitt SD et al. Phylogenetic classification of yeasts and related taxa within Pucciniomycotina. Stud Mycol 2015; 81: 149– 189 [CrossRef] [PubMed]
    [Google Scholar]
  9. Hibbett DS, Binder M, Bischoff JF, Blackwell M, Cannon PF et al. A higher-level phylogenetic classification of the fungi. Mycol Res 2007; 111: 509– 547 [CrossRef] [PubMed]
    [Google Scholar]
  10. Toome M, Roberson RW, Aime MC. Meredithblackwellia eburnea gen. et sp. nov., Kriegeriaceae fam. nov. and Kriegeriales ord. nov.–toward resolving higher-level classification in Microbotryomycetes. Mycologia 2013; 105: 486– 495 [CrossRef] [PubMed]
    [Google Scholar]
  11. Nakase T, Takashima M. A simple procedure for the high frequency isolation of new taxa of ballistosporous yeasts living on the surfaces of plants. RIKEN Rev 1993; 3: 33– 34
    [Google Scholar]
  12. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts: a Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011; pp. 87– 110 [Crossref]
    [Google Scholar]
  13. Makimura K, Murayama SY, Yamaguchi H. Detection of a wide range of medically important fungi by the polymerase chain reaction. J Med Microbiol 1994; 40: 358– 364 [CrossRef] [PubMed]
    [Google Scholar]
  14. Bai FY, Zhao JH, Takashima M, Jia JH, Boekhout T et al. Reclassification of the Sporobolomyces roseus and Sporidiobolus pararoseus complexes, with the description of Sporobolomyces phaffii sp. nov. Int J Syst Evol Microbiol 2002; 52: 2309– 2314 [CrossRef] [PubMed]
    [Google Scholar]
  15. Liu XZ, Wang QM, Theelen B, Groenewald M, Bai FY et al. Phylogeny of tremellomycetous yeasts and related dimorphic and filamentous basidiomycetes reconstructed from multiple gene sequence analyses. Stud Mycol 2015; 81: 1– 26 [CrossRef] [PubMed]
    [Google Scholar]
  16. Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 2013; 30: 772– 780 [CrossRef] [PubMed]
    [Google Scholar]
  17. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014; 30: 1312– 1313 [CrossRef] [PubMed]
    [Google Scholar]
  18. Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A et al. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 2012; 61: 539– 542 [CrossRef] [PubMed]
    [Google Scholar]
  19. Posada D. jModelTest: phylogenetic model averaging. Mol Biol Evol 2008; 25: 1253– 1256 [CrossRef] [PubMed]
    [Google Scholar]
  20. Librado P, Rozas J. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 2009; 25: 1451– 1452 [CrossRef] [PubMed]
    [Google Scholar]
  21. Huson DH, Bryant D. Application of phylogenetic networks in evolutionary studies. Mol Biol Evol 2006; 23: 254– 267 [CrossRef] [PubMed]
    [Google Scholar]
  22. Mašínová T, Pontes A, Carvalho C, Sampaio JP, Baldrian P. Libkindia masarykiana gen. et sp. nov., Yurkovia mendeliana gen. et sp. nov. and Leucosporidium krtinense f.a. sp. nov., isolated from temperate forest soils. Int J Syst Evol Microbiol 2017; 67: 902– 908 [CrossRef] [PubMed]
    [Google Scholar]
  23. Taylor JW, Jacobson DJ, Kroken S, Kasuga T, Geiser DM et al. Phylogenetic species recognition and species concepts in fungi. Fungal Genet Biol 2000; 31: 21– 32 [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002323
Loading
/content/journal/ijsem/10.1099/ijsem.0.002323
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error