1887

Abstract

A novel endophytic actinobacterium, designated strain 8BXZ-J1, was isolated from surface-sterilized branches of collected from Beilun Estuary Mangrove Forest National Nature Reserve in Guangxi, China, and examined by a polyphasic approach to determine its taxonomic position. Cells of the isolate were Gram-stain-positive, aerobic, non-spore-forming, non-motile and short rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 8BXZ-J1 belonged to the genus , sharing highest similarity with DSM 27140 (96.9 %). The isolate grew at 10–35 °C (optimum, 28–30 °C), at pH 6.0–8.0 (optimum, pH 7.0) and in the presence of 0–10 % (w/v) NaCl (optimum, 0–5.0 %). The organism contained -2,6-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, MK-8(H) as the major menaquinone, and a polar lipid profile including diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and two unknown lipids. The major fatty acids of strain 8BXZ-J1 were C 10-methyl, iso-C and C. The G+C content of the genomic DNA was 68.7 mol%. These data demonstrate that strain 8BXZ-J1 is representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 8BXZ-J1 (=KCTC 39789=CGMCC 1.16067).

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2017-11-01
2020-10-01
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