1887

Abstract

One unidentified, Gram-stain-positive, catalase-negative coccus-shaped organism was recovered from a subcutaneous abscess of the udder of a sheep and subjected to a polyphasic taxonomic analysis. Based on cellular morphology and biochemical criteria, the isolate was tentatively assigned to the genus Streptococcus , although the organism did not appear to match any recognized species. 16S rRNA gene sequence comparison studies confirmed its identification as a member of the genus Streptococcus and showed that the nearest phylogenetic relatives of the unknown coccus corresponded to Streptococcus moroccensis and Streptococcus cameli (95.9 % 16S rRNA gene sequence similarity). The sodA sequence analysis showed less than 89.3 % sequence similarity with the currently recognized species of the genus Streptococcus . The novel bacterial isolate was distinguished from close relatives of the genus Streptococcus by using biochemical tests. A mass spectrometry profile was also obtained for the novel isolate using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Based on both phenotypic and phylogenetic findings, it is proposed that the unknown bacterium be classified as a representative of a novel species of the genus Streptococcus , Streptococcus ovuberis sp. nov. The type strain of Streptococcus ovuberissp. nov. is VB15-00779 (=CECT 9179=CCUG 69612).

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2017-09-25
2019-10-24
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References

  1. Wilson CD, Salt GF. Streptococci in animal disease. Soc Appl Bacteriol Symp Ser 1978; 7: 143– 156 [PubMed]
    [Google Scholar]
  2. Chanter N. Streptococci and enterococci as animal pathogens. J Appl Microbiol 1997; 83: 100S– 109S [CrossRef] [PubMed]
    [Google Scholar]
  3. Las Heras A, Vela AI, Fernández E, Legaz E, Domínguez L et al. Unusual outbreak of clinical mastitis in dairy sheep caused by Streptococcus equi subsp. zooepidemicus. J Clin Microbiol 2002; 40: 1106– 1108 [CrossRef] [PubMed]
    [Google Scholar]
  4. Mørk T, Waage S, Tollersrud T, Kvitle B, Sviland S. Clinical mastitis in ewes; bacteriology, epidemiology and clinical features. Acta Vet Scand 2007; 49: 23 [CrossRef] [PubMed]
    [Google Scholar]
  5. Guerreiro O, Velez Z, Alvarenga N, Matos C, Duarte M. Molecular screening of ovine mastitis in different breeds. J Dairy Sci 2013; 96: 752– 760 [CrossRef] [PubMed]
    [Google Scholar]
  6. Kumar A, Verma AK, Sharma AK, Rahal A. Isolation and antibiotic sensitivity of Streptococcus pneumoniae infections with involvement of multiple organs in lambs. Pak J Biol Sci 2013; 16: 2021– 2025 [PubMed] [Crossref]
    [Google Scholar]
  7. Rutherford SJ, Rycroft AN, Ridler AL. Sources of Streptococcus dysgalactiae in English and Welsh sheep flocks affected by infectious arthritis (joint ill). Vet Rec 2014; 174: 579 [CrossRef] [PubMed]
    [Google Scholar]
  8. Rutherford SJ, Jeckel S, Ridler A. Characteristics of sheep flocks affected by Streptococcus dysgalactiae arthritis. Vet Rec 2015; 176: 435 [CrossRef] [PubMed]
    [Google Scholar]
  9. Muckle A, López A, Gottschalk M, López-Méndez C, Giles J et al. Isolation of Streptococcus suis from 2 lambs with a history of lameness. Can Vet J 2014; 55: 946– 949 [PubMed]
    [Google Scholar]
  10. Tadayon RA, Cheema AH, Muhammed SI. Microorganisms associated with abscesses of sheep and goats in the south of Iran. Am J Vet Res 1980; 41: 798– 802 [PubMed]
    [Google Scholar]
  11. Collins MD, Hutson RA, Hoyles L, Falsen E, Nikolaitchouk N et al. Streptococcus ovis sp. nov., isolated from sheep. Int J Syst Evol Microbiol 2001; 51: 1147– 1150 [Crossref]
    [Google Scholar]
  12. Al-Harbi KB. Prevalence and etiology of abscess disease of sheep and goats at Qassim region, Saudi Arabia. Vet World 2011; 4: 495– 499 [Crossref]
    [Google Scholar]
  13. Al-Harbi KB, Mahmoud OM. Abscess disease of sheep and goats: a disease of major concern in Saudi Arabia that urges production of an effective vaccine. J Agric Vet Sci 2012; 5: 61– 72
    [Google Scholar]
  14. Mugale Madhav N, Blachandran C, Dillibabu V, Kirubharan J, Dhinakar Raj G et al. Hepatic abscess in sheep and goat caused by O26 Escherichia coli serotype: an emerging pathogen. Indian Vet J 2015; 92: 76– 79
    [Google Scholar]
  15. Lawson PA, Gharbia SE, Shah HN, Clark DR. Recognition of Fusobacterium nucleatum subgroups Fn-1, Fn-2 and Fn-3 by ribosomal RNA gene restriction patterns. FEMS Microbiol Lett 1989; 53: 41– 45 [PubMed] [Crossref]
    [Google Scholar]
  16. Vela AI, Pérez-Sancho M, Zamora L, Domínguez L, Fernández-Garayzábal JF. Jeotgalibaca porci sp. nov. and Jeotgalibaca arthritidis sp. nov., two new species isolated from pigs and emended description of the genus Jeotgalibaca. Int J Syst Evol Microbiol 2017; 67: 1473– 1477 [Crossref]
    [Google Scholar]
  17. Hutson RA, Thompson DE, Collins MD. Genetic interrelationships of saccharolytic Clostridium botulinum types B, E and F and related clostridia as revealed by small-subunit rRNA gene sequences. FEMS Microbiol Lett 1993; 108: 103– 110 [CrossRef] [PubMed]
    [Google Scholar]
  18. Pascual C. Molecular taxonomy studies on some high G+C Gram-positive bacteria from human and animal sources. PhD Thesis University of Reading, United Kingdom: 1999
    [Google Scholar]
  19. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62: 716– 721 [CrossRef] [PubMed]
    [Google Scholar]
  20. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44: 846– 849 [CrossRef]
    [Google Scholar]
  21. Rasmussen SW. SeqTools, a Program Suite for Sequence Analysis Copenhagen: Carlsberg Laboratory; 2002
    [Google Scholar]
  22. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4: 406– 425 [PubMed]
    [Google Scholar]
  23. Page RD. TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 1996; 12: 357– 358 [PubMed]
    [Google Scholar]
  24. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28: 2731– 2739 [CrossRef] [PubMed]
    [Google Scholar]
  25. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16: 111– 120 [CrossRef] [PubMed]
    [Google Scholar]
  26. Poyart C, Quesne G, Coulon S, Berche P, Trieu-Cuot P. Identification of streptococci to species level by sequencing the gene encoding the manganese-dependent superoxide dismutase. J Clin Microbiol 1998; 36: 41– 47 [PubMed]
    [Google Scholar]
  27. Glazunova OO, Raoult D, Roux V. Streptococcus massiliensis sp. nov., isolated from a patient blood culture. Int J Syst Evol Microbiol 2006; 56: 1127– 1131 [CrossRef] [PubMed]
    [Google Scholar]
  28. Glazunova OO, Raoult D, Roux V. Partial sequence comparison of the rpoB, sodA, groEL and gyrB genes within the genus Streptococcus. Int J Syst Evol Microbiol 2009; 59: 2317– 2322 [CrossRef] [PubMed]
    [Google Scholar]
  29. Gonzalez JM, Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 2002; 4: 770– 773 [PubMed] [Crossref]
    [Google Scholar]
  30. Sokal RR, Rohlf FJ. Biometry, 2nd ed. New York: W.H. Freeman; 1981
    [Google Scholar]
  31. Facklam R, Elliott JA. Identification, classification, and clinical relevance of catalase-negative, gram-positive cocci, excluding the streptococci and enterococci. Clin Microbiol Rev 1995; 8: 479– 495 [PubMed]
    [Google Scholar]
  32. Vela AI, Perez Sancho M, Domínguez L, Busse H-J, Fernández-Garayzábal JF et al. Pelistega suis sp. nov., a new Pelistega species isolated from domestic and wild animals. Int J Syst Evol Microbiol 2015; 65: 4909– 4914 [Crossref]
    [Google Scholar]
  33. Alispahic M, Hummel K, Jandreski-Cvetkovic D, Nöbauer K, Razzazi-Fazeli E et al. Species-specific identification and differentiation of Arcobacter, Helicobacter and Campylobacter by full-spectral matrix-associated laser desorption/ionization time of flight mass spectrometry analysis. J Med Microbiol 2010; 59: 295– 301 [CrossRef] [PubMed]
    [Google Scholar]
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