1887

Abstract

One unidentified, Gram-stain-positive, catalase-negative coccus-shaped organism was recovered from a subcutaneous abscess of the udder of a sheep and subjected to a polyphasic taxonomic analysis. Based on cellular morphology and biochemical criteria, the isolate was tentatively assigned to the genus , although the organism did not appear to match any recognized species. 16S rRNA gene sequence comparison studies confirmed its identification as a member of the genus and showed that the nearest phylogenetic relatives of the unknown coccus corresponded to and (95.9 % 16S rRNA gene sequence similarity). The sequence analysis showed less than 89.3 % sequence similarity with the currently recognized species of the genus . The novel bacterial isolate was distinguished from close relatives of the genus by using biochemical tests. A mass spectrometry profile was also obtained for the novel isolate using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Based on both phenotypic and phylogenetic findings, it is proposed that the unknown bacterium be classified as a representative of a novel species of the genus , sp. nov. The type strain of sp. nov. is VB15-00779 (=CECT 9179=CCUG 69612).

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2017-11-01
2020-09-23
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