1887

Abstract

Two Gram-stain-negative, aerobic, non-motile, non-spore-forming and rod-shaped bacteria, designated strains T2 and T5, were isolated from a culture of Microcystis from Daejeon, Republic of Korea. Comparative 16S rRNA gene sequence studies placed the new isolates in the class Alphaproteobacteria and, notably, most closely related to Blastomonas aquatica PE 4-5, Blastomonas natatoria DSM 3183 and Blastomonas ursincola KR-99 showing 99.4 %, 98.2 % and 97.9 % 16S rRNA gene sequence similarities, respectively. The two novel strains shared 100 % similarity with each other. The cells of strains T2 and T5 formed yellow colonies on R2A agar and contained Q-10 as the only ubiquinone, sphingoglycolipid, phosphatidylethanolamine, phosphatidylcholine, and phosphatidylglycerol as major polar lipids, and C17 : 1ω6c, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C17 : 1ω8c and C17 : 0 as the major fatty acids (>5 %). The DNA G+C content of the genomes was determined to be 64.2 mol% for strain T2 and 64.4 mol% for strain T5. The DNA–DNA hybridization values of strains T2 and T5 with B. aquatica PE 4-5, B. natatoria DSM 3183, and B. ursincola KR-99 were 19.7–42.4 %. Based on the combined genotypic and phenotypic data, we propose that strains T2 and T5 represent a novel species of the genus Blastomonas , for which the name Blastomonas fulvasp. nov. is proposed. The type strain is T2 (=KCTC 42354=JCM 30467).

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2017-08-18
2019-10-14
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