- Volume 67, Issue 8, 2017

Strain AFT2T was isolated from a mural painting sample from a ca. 1500-year-old tomb located in Shanxi Province, China. The isolate was a Gram-stain-positive, non-motile, non-spore-forming, aerobic and oval to short-rod-shaped bacterium that formed white-pigmented colonies. Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain AFT2T was most closely (97.01 %) correlated and formed a monophyletic clade with Naumannella halotolerans WS4616T (=DSM 24323T). The G+C content of the genomic DNA was 71.97 mol%, and the strain showed 37.27 % DNA–DNA relatedness to N. halotolerans DSM 24323T. The major cellular fatty acid was anteiso-C15 : 0 (55.32 %), and MK-9(H4) was the only respiratory quinone. The polar lipids comprised phosphatidylglycerol, diphosphatidylglycerol, two unknown phospholipids and five unknown glycolipids. ll-Diaminopimelic acid was detected in the cell-wall peptidoglycan (type A3γ), and the whole-cell sugars consisted of ribose, mannose, arabinose and galactose. On the basis of its phenotypic and phylogenetic characteristics, it is proposed that strain AFT2T should be classified as a representative of a novel species of the genus Naumannella , for which the name Naumannella cuiyingiana sp. nov. is proposed. The type strain is AFT2T (=CCTCC AB 2015428T=DSM 103164T).

On the basis of whole genome comparisons of Streptomyces griseorubiginosus and Streptomyces phaeopurpureus it could by shown that these two species are subjective synonyms. The names of both species have been published in the Approved Lists of Bacterial Names and, in such a case, normally Rule 24b (1) of the Prokaryotic Code applies, which reads: ‘If two names compete for priority and if both names date from 1 January 1980 on an Approved List, the priority shall be determined by the date of the original publication of the name before 1 January 1980’. Streptomyces griseorubiginosus and Streptomyces phaeopurpureus were both effectively published in 1957, and for both publications, the exact date cannot be obtained. In this case a further statement of Rule 24 applies, which reads: ‘If the names or epithets are of the same date, the author who first unites the taxa has the right to choose one of them, and his choice must be followed.’ Hence we propose that Streptomyces phaeopurpureus is a later heterotypic subjective synonym of Streptomyces griseorubiginosus .

A Gram-staining-positive, aerobic non-spore-forming and short-rod-shaped endophytic actinomycete was isolated from a branch of Sonneratia apetala, designated strain BGMRC0092T and investigated in detail data to determine its taxonomic position. On the basis of the 16S rRNA gene sequence analysis, the results of the phylogenetic analyses indicated that BGMRC0092T was most closely related to Nocardioides alpinus Cr7-14T (96.9 %), Nocardioides oleivorans DSM16090T (96.4 %) and Nocardiodes exalbidus RC825T (96.3 %). The predominant cellular fatty acids of BGMRC0092T were iso-C16 : 0 and C18 : 1ω8c. The major menaquinone was MK-8(H4). The diagnostic diamino acid in the cell-wall peptidoglycan was ll-2,6-diaminopimelic acid. The predominant cell-wall sugars were composed of galactose, mannose, rhamnose and xylose. The polar lipid pattern contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, one unknown phospholipid and three unknown polar lipids. The DNA G+C content was 69.3 mol%. On the basis of phenotypic and chemotaxonomic characteristics and the results of phylogenetic analysis, BGMRC0092T represents a novel species of the genus Nocardioides , for which the name Nocardioides sonneratiae sp. nov. is proposed. The type strain is Nocardioides sonneratiae BGMRC0092T (=KCTC 39565T=NBRC 110251 T=DSM 100390T).

A Gram-stain-positive bacterium, designated TSL3T, was isolated from faeces of a porcupine, Hystrix indica, from the Budapest Zoo and Botanical Garden, Hungary. On the basis of 16S rRNA gene sequence analysis, the strain is phylogenetically related to the family Micrococcaceae . The highest 16S rRNA gene sequence similarity was found with Micrococcus terreus V3M1T (96.50 %) followed by Arthrobacter humicola KV-653T (96.43 %). Cells of strain TSL3T were aerobic, non-motile and coccoid-shaped. The main fatty acids were anteiso-C15 : 0 (54.4 %), iso-C16 : 0 (18.2 %) and iso C15 : 0 (9.7 %). The major menaquinone was MK-7, and the polar lipid profile included phosphatidylglycerol, diphosphatidylglycerol, dimannosylglyceride, trimannosyldiacylglycerol, phosphatidylinositol, three unknown phospholipids and two unknown glycolipids. Strain TSL3T showed the peptidoglycan structure A4alpha l-Lys - Gly - l-Glu. The DNA G+C content of strain TSL3T was 58.4 mol%. Phenotypic and genotypic characterisation clearly showed that strain TSL3T could be differerentiated from the members of other genera in the family Micrococcaceae . According to these results, strain TSL3T represents a novel genus and species, for which the name Micrococcoides hystricis gen. nov., sp. nov. is proposed. The type strain is TSL3T (=DSM 29785T=NCAIM B. 02604T).

A novel actinomycete, designated strain EURKPP3H10T, was isolated from surface-sterilized roots of Eucalyptus camaldulensis Dehnh., collected from Kamphaengphet Silvicultural Research Station, Kamphaengphet province, Thailand. The taxonomic position of strain EURKPP3H10T was studied using a polyphasic approach. Phylogenetic evaluation based on 16S rRNA gene sequence analysis showed that strain EURKPP3H10T belongs to the genus Cryptosporangium , with the highest sequence similarity to Cryptosporangium cibodasense LIPI11-2-Ac046T (99.2 %). Colonies of strain EURKPP3H10T were orange yellow. Spherical sporangia with motile spores were observed. The strain contained meso-diaminopimelic acid and acofriose, arabinose, galactose, glucose, mannose, xylose and ribose in whole-cell hydrolysates. The predominant menaquinones were MK-9(H8) and MK-9(H6). The major fatty acids were iso-C16 : 0, C17 : 1ω8c, C18 : 1ω9c and C17 : 0. The polar lipids of the strain were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and unknown lipids. The DNA G+C content of the genomic DNA was 71.5 mol%. Based on comparative analysis of physiological, biochemical and chemotaxonomic data, including DNA–DNA hybridization, strain EURKPP3H10T represents a novel species of the genus Cryptosporangium , for which the name Cryptosporangium eucalypti sp. nov. is proposed. The type strain is EURKPP3H10T (=BCC 77605T=NBRC 111482T).

The taxonomic positions of Microterricola viridarii JCM 15926T, Phycicola gilvus DSM 18319T and Leifsonia pindariensis JCM 15132T were re-examined. Phylogenetic analysis and 16S rRNA gene sequence similarities revealed that all three strains are closely related with each other and form a monophyletic cluster with high sequence similarity (99.2 –99.9 %). A dendrogram constructed based on the protein spectra generated by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy also displayed close clustering of these three strains. The fatty acid profiles of three strains were very similar to each other and contained branched fatty acids (anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0) as the predominant cellular fatty acids. The polar lipid profiles of the three stains were similar and consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine as major polar lipids and an unknown lipid. Comparisons of morphological, chemotaxonomic and physiological data of Microterricola viridarii JCM 15926T, Leifsonia pindariensis JCM 15132T and Phycicola gilvus DSM 18319T are in agreement with the features of a common genus. DNA–DNA hybridization data generated during this study showed less than 70 % reassociation value with each other indicating that they are different at species level. Based on the present study, we conclude that Phycicola gilvus DSM 18319T and Leifsonia pindariensis JCM 15132T should be reclassified under the genus Microterricola , since this genus has the nomenclatural priority, and reclassified as Microterricola gilva comb. nov. (type strain SSWW-21T=DSM 18319T=KCTC 19185T=JCM 30550T) and Microterricola pindariensis comb. nov. (type strain PON10T=LMG 24222T=JCM 15132T=MTCC9128T). An emended description of the genus Microterricola is also presented.

A novel slowly growing, non-chromogenic species of the class Actinobacteria was isolated from a human respiratory sample in Nebraska, USA, in 2012. Analysis of the internal transcribed spacer sequence supported placement into the genus Mycobacterium with high sequence similarity to a previously undescribed strain isolated from a patient respiratory sample from Oregon, USA, held in a collection in Colorado, USA, in 2000. The two isolates were subjected to phenotypic testing and whole genome sequencing and found to be indistinguishable. The bacteria were acid-fast stain-positive, rod-shaped and exhibited growth after 7–10 days on solid media at temperatures ranging from 25 to 42°C. Colonies were non-pigmented, rough and slightly raised. Analyses of matrix-assisted laser desorption ionization time-of-flight profiles showed no matches against a reference library of 130 mycobacterial species. Full-length 16S rRNA gene sequences were identical for the two isolates, the average nucleotide identity (ANI) between their genomes was 99.7 % and phylogenetic comparisons classified the novel mycobacteria as the basal most species in the slowly growing Mycobacterium clade. Mycobacterium avium is the most closely related species based on rpoB gene sequence similarity (92 %), but the ANI between the genomes was 81.5 %, below the suggested cut-off for differentiating two species (95 %). Mycolic acid profiles were more similar to M. avium than to Mycobacterium simiae or Mycobacterium abscessus . The phenotypic and genomic data support the conclusion that the two related isolates represent a novel Mycobacterium species for which the name Mycobacterium talmoniae sp. nov. is proposed. The type strain is NE-TNMC-100812T (=ATCC BAA-2683T=DSM 46873T).

The Mycobacterium abscessus complex is a group of rapidly growing, multiresistant mycobacteria previously divided into three species. Proposal for the union of Mycobacterium bolletii and Mycobacterium massiliense into one subspecies, so-called M. abscessus subsp. massiliense, created much confusion about the routine identification and reporting of M. abscessus clinical isolates for clinicians. Results derived from multigene sequencing unambiguously supported the reinstatement of M. massiliense and M. bolletii as species, culminating in the presence of erm(41)-encoded macrolide resistance in M. bolletii . Present genome-based analysis unambiguously supports the reinstatement of M. massiliense and M. bolletii as species after the average nucleotide identity values of 96.7 % for M. abscessus versus M. bolletii , and 96.4 % for M. abscessus versus M. massiliense , and the 96.6 % identity between M. bolletii and M. massiliense was put into the perspective of a larger, 28-species analysis. Accordingly, DNA–DNA hybridization values predicted by the complete rpo B gene sequencing analysis were between 68.7 and 72.3 % in this complex. These genomic data as well as the phenotypic characteristics prompted us to propose to reinstate the previously known M. massiliense and M. bolletii into two distinct species among the M. abscessus complex.

A previously undescribed rapidly growing, non-pigmented mycobacterium was identified based on biochemical and nucleic acid analyses, as well as growth characteristics. Seven isolates were cultured from samples collected from five thread-sail filefish (Stephanolepis cirrhifer) and two farmed black scraper (Thamnaconus modestus). Bacterial growth occurred at 15–35 °C on Middlebrook 7H11 agar. The bacteria were positive for catalase activity at 68 °C and urease activity, intermediate for iron uptake, and negative for Tween 80 hydrolysis, nitrate reduction, semi-quantitative catalase activity and arylsulfatase activity at day 3. No growth was observed on Middlebrook 7H11 agar supplemented with picric acid, and very little growth was observed in the presence of 5 % NaCl. α- and α′-mycolates were identified in the cell walls, and a unique profile of the fatty acid methyl esters and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiles of the protein and cell-wall lipids were acquired. Sequence analysis revealed that the seven isolates shared identical sequences for the 16S rRNA, rpoB, hsp65, recA and sodA genes. Phylogenetic analysis of the five gene sequences confirmed that the isolates were unique, but closely related to Mycobacterium chelonae . Antibiotic susceptibility testing revealed the minimum inhibitory concentration (MIC) of clarithromycin against this novel species was <0.25 µg ml−1, which was lower than that for Mycobacterium salmoniphilum . The hsp65 PCR restriction enzyme analysis pattern differed from those of M. chelonae and M. salmoniphilum . Based on these findings, the name Mycobacterium stephanolepidis sp. nov. is proposed for this novel species, with the type strain being NJB0901T (=JCM 31611T=KCTC 39843T).

A Gram-stain-positive actinobacterium, designated as strain 2-36T, was isolated from sludge of a sewage outlet in a manganese mine. Phylogenetic analysis of 16S rRNA genes assigned strain 2-36T in a single lineage in the family Geodermatophilaceae and closely related to Modestobacter roseus KLBMP 1279T (93.8 % similarity), Blastococcus saxobsidens BC444T (93.1 %) and Geodermatophilus aquaeductus BMG801T (92.8 %). It contained iso-C16 : 0, iso-C15 : 0, C17 : 1ω6c, iso-C14 : 0 and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) as the major fatty acids (>5 %), with MK-9(H4), MK-8(H4) and MK-9(H6) as the quinones. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid and the DNA G+C content was 70.1 mol%. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, an unknown glycolipid, an unknown aminophospholipid and two unknown phospholipids. Compared to closely related strains, strain 2-36T showed distinguishing characteristics, such as the distinct phylogenetic lineage, positive result for phenylacetic acid assimilation and negative result for α-glucosidase and having C17 : 1ω6c and iso-C14 : 0 as the major fatty acids. On the basis of the polyphasic analyses, strain 2-36T represents a novel species of a new genus within the family Geodermatophilaceae, for which the name Cumulibacter manganitolerans gen. nov., sp. nov. is proposed. The type strain of 'Cumulibacter manganitolerans' is 2-36T (=CCTCC AA 2016026T=DSM 103787T).

During isolation of soil bacteria having antibacterial functions, an aerobic, Gram-stain-positive, oxidase-negative, catalase-positive bacterium, designated strain R434T, was isolated. Strain R434T showed antimicrobial activity against Propionibacterium acnes and Staphylococcus epidermidis and significant enzyme-inhibitory capability. The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid, and the whole-cell sugars were galactose, arabinose and glucose. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain R434T formed a lineage within the family Pseudonocardiaceae . Strain R434T showed highest sequence similarity with type strains of the genus Actinokineospora , including Actinokineospora guangxiensis Gk-6T (99.4 % sequence similarity), Actinokineospora soli YIM 75948T (98.5 %), Actinokineospora fastidiosa IMSNU 20054T (98.0 %), Actinokineospora cibodasensis ID03-0784T (97.9 %), Actinokineospora terrae IFO 15668T (97.6 %) and Actinokineospora auranticolor IFO 16518T (97.4 %). The predominant respiratory quinone of strain R434T was MK-9(H4). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and unidentified glycolipid. The major cellular fatty acids were iso-C16 : 0, iso-C16 : 1 H and C17 : 1ω6c. The DNA G+C content of strain R434T was 71.6 mol%. On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, strain R434T represents a novel species of the genus Actinokineospora , for which the name Actinokineospora acnipugnans sp. nov. is proposed. The type strain of ' Actinokineospora acnipugnans' is R434T (=KEMB 9005-403T=KACC 18904T=JCM 31557T).

A novel Gram-stain-positive bacterium designated CMU-NKS-70T was isolated from a subterranean termite nest and characterized using a polyphasic approach. The strain exhibited branching, pinkish-cream aerial mycelium and cream–brown substrate mycelium, and formed chains of rod-like spores. The 16S rRNA gene sequence analyses indicated that strain CMU-NKS-70T belonged to the genus Pseudonocardia , showing high similarity with Pseudonocardia oroxyli D10T (98.9 % 16S rRNA gene sequence similarity), Pseudonocardia xishanensis YIM 63638T (98.9 %) and Pseudonocardia kujensis A4038T (98.5 %). However, DNA–DNA relatedness values between strains CMU-NKS-70T and the closest phylogenetically related species ranged from 40.5±2.9 to 48.6±0.7 %. Whole-cell hydrolysates of strain CMU-NKS-70T consisted of meso-diaminopimelic acid, glucose, galactose, arabinose, mannose, ribose and rhamnose. The predominant menaquinone was MK-8(H4). The major cellular fatty acids (>10 %) were iso-C16 : 0, C16 : 0, C16 : 1ω7c and/or iso-C15 : 0 2-OH and 10-methyl C16 : 0. The polar lipids detected were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, three unidentified glycolipids and two unidentified phospholipids. The G+C content of genomic DNA was 71.9 mol%. The physiological and biochemical properties also supported the phenotypic distinction of strain CMU-NKS-70T from its closely related species. On the basis of evidence from this study using a polyphasic approach, strain CMU-NKS-70T represents a novel species of the genus Pseudonocardia for which the name Pseudonocardia thailandensis sp. nov. is proposed. The type strain is CMU-NKS-70T (=JCM 31292T=TBRC 2000T).

A cold-tolerant, translucent, yellow-pigmented, Gram-stain-positive, non-motile, rod-shaped bacteria was isolated from snow of the Zadang Glacier on the Tibetan Plateau, PR China. 16S rRNA gene sequence similarity analysis indicated that the isolate was closely related to Conyzicola lurida KCTC 29231T and Leifsonia psychrotolerans DSM 22824T at a level of 97.72 and 97.49 %, respectively. Other close relatives had a 16S rRNA gene sequence similarity of less than 97 %. The major cell-wall amino acid was 2,4-diaminobutyric acid. The cell-wall sugars were rhamnose, ribose, glucose, galactose and mannose. The major menaquinones were MK-9, MK-10 and MK-11. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The predominant fatty acids were anteiso-C15 : 0 and iso-C16 : 0. In DNA–DNA hybridization tests, strain ZD5-4T shared 43.6 and 34.2 % relatedness with C. lurida KCTC 29231T and L. psychrotolerans DSM 22824T, respectively. Based on phenotypic and chemotaxonomic characteristics, strain ZD5-4T was considered to represent a novel species of the genus Conyzicola , for which the name Conyzicola nivalis sp. nov. is proposed. The type strain is ZD5-4T (=JCM 30076T=CGMCC 1.12813T). Emended descriptions of the genus Conyzicola and Conyzicola lurida are also provided.

Halopolyspora alba AFM10251T was proposed to represent a novel species of a new genus belonging to the family Actinopolysporaceae in a previous study. The family Mzabimycetaceae , containing one genus, Mzabimyces , was proposed subsequently and Mzabimyces algeriensis H195T was the type strain. However, analysis of 16S rRNA gene sequence similarity showed that the two strains were highly similar (99.2 %). Phenotypic and chemotaxonomic data, as well as DNA–DNA hybridization confirmed that the two strains are different genomic species of the same genus, Halopolyspora . Mzabimyces algeriensis should be classified in the genus Halopolyspora as Halopolyspora algeriensis comb. nov. The type strain of Halopolyspora algeriensis is H195T (=DSM 46680T=CECT 8575T).

A slightly irregular, short rod-shaped bacterial strain, MOZIV/2T, showing activity of fructose 6-phosphate phosphoketolase was isolated from the oral cavity of a home-bred guinea-pig. Based on comparative 16S rRNA gene sequence analyses, its closest relatives were Alloscardovia omnicolens DSM 21503T and Alloscardovia criceti DSM 17774T with 96.0 and 95.6 % pairwise similarities, respectively. Completeness of the compared sequences was 97.3 and 96.9 %, respectively. Growth was found only under anaerobic conditions. Activities of α- and β-gluco(galacto)sidases were detected in strain MOZIV/2T, which is characteristic for almost all members of the family Bifidobacteriaceae . Sequencing of other molecular markers (fusA, gyrB and xfp) revealed low gene sequence similarities to A. omnicolens DSM 21503T ranging from 72.7 to 87.5 %. Strain MOZIV/2T differed from other species within the genus Alloscardovia by the presence of C18 : 1ω9t. In addition, much higher proportions of C8 : 0, C11 : 0, C12 : 0, C14 : 1, C16 : 1 and C17 : 0 fatty acids were found in cells of strain MOZIV/2T. The peptidoglycan structure was of type A4α [l-Lys(l-Orn)–d-Asp], which is consistent with its classification within the genus Alloscardovia . The DNA G+C content (45.8 mol%) was lower than those found in other alloscardovia. Phylogenetic studies and evaluation of phenotypic characteristics including the results of biochemical, physiological and chemotaxonomic analyses confirmed the novel species status for strain MOZIV/2T, for which the name Alloscardovia venturai sp. nov. is proposed. The type strain is MOZIV/2T (=DSM 100237T=CCM 8604T=LMG 28781T).

One strain, designated 63MJ-1T, was isolated from fresh faeces of broad-winged katydids collected in Jinan-gun, Jeollabuk-do, the Republic of Korea. The organism stained Gram-positive and was an aerobic, non-flagellated and short-rod-shaped bacterium. The organism grew in the range of 4–35 °C (optimum, 28–30 °C) and pH 6.0–9.0 (optimum, pH 7.0), and in the presence of 5 % NaCl (w/v), but not in media containing 7 % NaCl. According to the 16S rRNA gene sequence analysis, strain 63MJ-1T showed the highest sequence similarities with Nakamurella panacisegetis P4-7T (95.9 %), Nakamurella endophytica 2Q3S-4-2T (95.8 %) and Nakamurella multipartita DSM 44233T (95.7 %). Phylogenetic trees also indicated that strain 63MJ-1T formed one robust cluster with members of the genus Nakamurella . The predominant quinone of strain 63MJ-1T was MK-8(H4). Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified aminophospholipid and two unidentified lipids. The major fatty acids were C16 : 0, anteiso-C15 : 0 and iso-C15 : 0. The peptidoglycan type was A1γ with meso-diaminopimelic acid as the diagnostic amino acid. The DNA G+C content was 64.6 mol%. Based on the phylogenetic, physiological and chemotaxonomic data, it was demonstrated that strain 63MJ-1T represents a novel species of the genus Nakamurella , for which the name Nakamurella intestinalis sp. nov. is proposed. The type strain is 63MJ-1T (=KACC 18662T=NBRC 111844T).

A novel actinobacterial strain, designated YIM A1135T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were aerobic, Gram-stain-positive and partially acid-alcohol-fast. Strain YIM A1135T shared 98.3 % 16S rRNA gene sequence similarity with Nocardia jejuensis NBRC 103114T and 97.6 % with Nocardia alba YIM 30243T. DNA–DNA hybridization values between strain YIM A1135T and related type strains of the genus Nocardia were less than 70 %. In addition, meso-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan. The whole-cell sugars were fructose, mannose, galactose and glucose. The major isoprenoid quinone was MK-8(H4, ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c, C18 : 0 10-methyl and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. Mycolic acids were present. The genomic DNA G+C content of strain YIM A1135T was 66.7 mol%. Based on the results of the molecular studies supported by its morphological, physiological, chemotaxnomic and other differential phenotypic characteristics, strain YIM A1135T is considered to represent a novel species within the genus Nocardia , for which the name Nocardia cavernae sp. nov. is proposed. The type strain is YIM A1135T (=KCTC 39595T=CCTCC AA 2017030T).

A novel endophytic actinomycete, designated strain SC1-1T, was isolated from sterilized stem tissue from Stahlianthus campanulatus collected in Udon Thani province, Thailand. The isolate formed short chains of spores on aerial mycelium and presented meso-diaminopimelic acid in the cell wall peptidoglycan. Glucose, madurose, mannose, rhamnose and ribose were observed as sugars in the cells. The cell membrane phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxy-phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and ninhydrin-positive phosphoglycolipids. The major menaquinones were MK-9(H4) and MK-9(H2). The main cellular fatty acids were iso-C16:0, 10-methyl C17 : 0 and C17 : 1ω6c. A high G+C content (70.7 mol%) was present in the genomic DNA. The taxonomic position based on the 16S rRNA gene sequence analysis revealed that strain SC1-1T belonged to the genus Nonomuraea and shared the highest 16S rRNA gene sequence similarity value with Nonomuraea dietziae DSM 44320T (98.82 %), followed by Nonomuraea africana IFO 14745T (98.58 %), Nonomuraea jabiensis A4036T (98.43 %), Nonomuraea endophytica YIM 65601T (98.36 %), Nonomuraea purpurea 1SM4-01T (98.34 %), Nonomuraea angiospora IFO 13155T (98.29 %), Nonomuraea roseola IFO 14685T (98.23 %) and Nonomuraea recticatena IFO 14525T (98.23 %). On the basis of the DNA–DNA hybridization relatedness and including the physiological and biochemical characteristics, strain SC1-1T should be judged as a novel species of the genus Nonomuraea , for which the name Nonomuraea stahlianthi sp. nov. is proposed. The type strain is strain SC1-1T (=BCC 66361T=NBRC 110006T).