1887

Abstract

During isolation of soil bacteria having antibacterial functions, an aerobic, Gram-stain-positive, oxidase-negative, catalase-positive bacterium, designated strain R434, was isolated. Strain R434 showed antimicrobial activity against Propionibacterium acnes and Staphylococcus epidermidis and significant enzyme-inhibitory capability. The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid, and the whole-cell sugars were galactose, arabinose and glucose. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain R434 formed a lineage within the family Pseudonocardiaceae . Strain R434 showed highest sequence similarity with type strains of the genus Actinokineospora , including Actinokineospora guangxiensis Gk-6 (99.4 % sequence similarity), Actinokineospora soli YIM 75948 (98.5 %), Actinokineospora fastidiosa IMSNU 20054 (98.0 %), Actinokineospora cibodasensis ID03-0784 (97.9 %), Actinokineospora terrae IFO 15668 (97.6 %) and Actinokineospora auranticolor IFO 16518 (97.4 %). The predominant respiratory quinone of strain R434 was MK-9(H4). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and unidentified glycolipid. The major cellular fatty acids were iso-C16 : 0, iso-C16 : 1 H and C17 : 1ω6c. The DNA G+C content of strain R434 was 71.6 mol%. On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, strain R434 represents a novel species of the genus Actinokineospora , for which the name Actinokineospora acnipugnans sp. nov. is proposed. The type strain of ' Actinokineospora acnipugnans' is R434 (=KEMB 9005-403=KACC 18904=JCM 31557).

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2017-08-18
2019-10-14
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