1887

Abstract

Four yeast strains were isolated from rotting wood samples collected in the Baotianman Nature Reserve in Henan Province, central China. The sequences of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer regions showed that these four strains represent two different undescribed yeast species belonging to the Ogataea clade. Ogataea neixiangensis sp. nov. produces two to four hat-shaped ascospores per ascus, and its closest relative among recognized species is Candida nitratophila. Ogataea paraovalis f.a., sp. nov. is closely related to Candida ovalis but the formation of ascospores was not observed on various sporulation media. The type strain of O. neixiangensis sp. nov. (MycoBank number MB 820697) is NYNU 16951 (=CICC 33166=CBS 14695), and the type strain of O. paraovalis f.a., sp. nov. (MycoBank number MB 820698) is NYNU 167106 (=CICC 33168=CBS 14697).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002075
2017-08-18
2019-09-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/8/3038.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002075&mimeType=html&fmt=ahah

References

  1. Yamada Y, Maeda K, Mikata K. The phylogenetic relationships of the hat-shaped ascospore-forming, nitrate-assimilating Pichia species, formerly classified in the genus Hansenula Sydow et Sydow, based on the partial sequences of 18S and 26S ribosomal RNAs (Saccharomycetaceae): the proposals of three new genera, Ogataea, Kuraishia, and Nakazawaea. Biosci Biotechnol Biochem 1994;58:1245–1257 [CrossRef][PubMed]
    [Google Scholar]
  2. Kurtzman CP, Robnett CJ. Systematics of methanol assimilating yeasts and neighboring taxa from multigene sequence analysis and the proposal of Peterozyma gen. nov., a new member of the Saccharomycetales. FEMS Yeast Res 2010;10:353–361 [CrossRef][PubMed]
    [Google Scholar]
  3. Nakase T, Imanishi Y, Ninomiya S, Takashima M. Candida rishirensis sp. nov., a novel methylotrophic anamorphic yeast species isolated from soil on Rishiri Island in Japan. J Gen Appl Microbiol 2010;56:169–173 [CrossRef][PubMed]
    [Google Scholar]
  4. Péter G, Dlauchy D, Tornai-Lehoczki J, Gouliamova D, Kurtzman CP. Ogataea saltuana sp. nov., a novel methanol-assimilating yeast species. Antonie van Leeuwenhoek 2011;100:375–383 [CrossRef][PubMed]
    [Google Scholar]
  5. Čadež N, Dlauchy D, Raspor P, Péter G. Ogataea kolombanensis sp. nov., Ogataea histrianica sp. nov. and Ogataea deakii sp. nov., three novel yeast species from plant sources. Int J Syst Evol Microbiol 2013;63:3115–3123 [CrossRef][PubMed]
    [Google Scholar]
  6. Limtong S, Kaewwichian R, Groenewald M. Ogataea kanchanaburiensis sp. nov. and Ogataea wangdongensis sp. nov., two novel methylotrophic yeast species from phylloplane in Thailand. Antonie van Leeuwenhoek 2013;103:551–558 [CrossRef][PubMed]
    [Google Scholar]
  7. Santos AR, Faria ES, Lachance MA, Rosa CA. Ogataea mangiferae sp. nov., a methylotrophic yeast isolated from mango leaves. Int J Syst Evol Microbiol 2015;65:1855–1859 [CrossRef][PubMed]
    [Google Scholar]
  8. Kurtzman CP. Ogataea Yamada Y, Maeda K & Mikata (1994). In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts – A Taxonomic Study, 5th ed.vol. 2 Amsterdam: Elsevier; 2011; pp.645–671[CrossRef]
    [Google Scholar]
  9. Lachance MA, Boekhout T, Scorzetti G, Fell JW, Kurtzman CP et al. Candida Berkhout (1923). In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts – A Taxonomic Study, 5th ed.vol. 2 Amsterdam: Elsevier; 2011; pp.987–1278[CrossRef]
    [Google Scholar]
  10. Fall R, Benson AA. Leaf methanol — the simplest natural product from plants. Trends Plant Sci 1996;1:296–301 [CrossRef]
    [Google Scholar]
  11. Hui FL, Chen L, Li ZH, Niu QH, Ke T. Metschnikowia henanensis sp. nov., a new anamorphic yeast species isolated from rotten wood in China. Antonie van Leeuwenhoek 2013;103:899–904 [CrossRef][PubMed]
    [Google Scholar]
  12. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts – A Taxonomic Study, 5th ed.vol. 1 Amsterdam: Elsevier; 2011; pp.87–110[CrossRef]
    [Google Scholar]
  13. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998;73:331–371 [CrossRef][PubMed]
    [Google Scholar]
  14. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: A Guide to Methods and Applications. New York: Academic Press 1990; pp.315–322
    [Google Scholar]
  15. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25:3389–3402 [CrossRef][PubMed]
    [Google Scholar]
  16. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  17. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  18. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  19. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002075
Loading
/content/journal/ijsem/10.1099/ijsem.0.002075
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error