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Abstract

A bacterial strain designated LYH-12 was isolated from a freshwater fish culture pond in Taiwan, ROC and characterized by taking a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain LYH-12 belonged to the genus Hymenobacter and was most closely related to Hymenobacter xinjiangensis X2-1g and Hymenobacter rigui WPCB131 with a sequence similarity of 96.6 % and less than 96.5 % with other members of the genus. Cells of strain LYH-12 were Gram-stain-negative, aerobic, non-motile rods that were covered by large capsules and formed light pink-coloured colonies. Growth occurred at 10–37 °C (optimum, 20–30 °C), at pH 6.5–7.5 (optimum, pH 7) and with 0–1 % NaCl (optimum, 0.5 %). Strain LYH-12 contained iso-C15 : 0, C16 : 1ω5c, C16 : 0, iso-C17 : 0 3-OH, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and anteiso-C17 : 1ω9c as the predominant fatty acids. The only isoprenoid quinone detected was MK-7. The polar lipid profile consisted of phosphatidylethanolamine, one uncharacterized aminophospholipid, four uncharacterized aminolipids, two uncharacterized phospholipids and three uncharacterized lipids. The major polyamine was homospermidine. The DNA G+C content of the genomic DNA was 64.3 mol%. On the basis of the phylogenetic inference and phenotypic data, strain LYH-12 should be classified as a novel species, for which the name Hymenobacter pallidus sp. nov. is proposed. The type strain is LYH-12 (=BCRC 80919=LMG 29171=KCTC 42898).

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2017-08-18
2019-08-18
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