1887

Abstract

A slightly thermophilic, aerobic bacterium, designated YIM 72257, was isolated from a sediment sample taken from a hot spring in Tengchong county, Yunnan province, south-west China. The isolate was Gram-stain-negative, non-sporulating and forms non-motile rods, appearing in chains. The isolate grew at 50–65 °C, pH 6.0–9.0 and with 0.5–1 % NaCl (w/v). 16S rRNA gene sequence analysis showed that strain YIM 72257 was most closely related to LY1 (95.6 %), DSM 1279 (95.1 %) and WR-30 (94.6 %). The genomic DNA G+C content of strain YIM 72257 was 62.6 mol%. The main cellular fatty acids (>5 %) were anteiso-C, anteiso-C, iso-C, iso-C, iso-C and C. The polar lipids consisted of an uncharacterized phospholipid and two glycolipids. Based on phenotypic, phylogenetic and chemotaxonomic characteristics, strain YIM 72257 is proposed to be a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 72257 (=KCTC 52599=CCTCC AB 2017100).

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2017-08-01
2021-07-28
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References

  1. Nobre MF, Truper HG, da Costa MS. Transfer of Thermus ruber (Loginova et al. 1984), Thermus silvanus (Tenreiro et al. 1995), and Thermus chliarophilus (Tenreiro et al. 1995) to Meiothermus gen. nov. as Meiothermus ruber comb. nov., Meiothermus silvanus comb. nov., and Meiothermus chliarophilus comb. nov., respectively, and emendation of the genus Thermus. Int J Syst Bacteriol 1996; 46:604–606 [View Article]
    [Google Scholar]
  2. Chung AP, Rainey F, Nobre MF, Burghardt J, da Costa MS. Meiothermus cerbereus sp. nov., a new slightly thermophilic species with high levels of 3-hydroxy fatty acids. Int J Syst Bacteriol 1997; 47:1225–1230 [View Article][PubMed]
    [Google Scholar]
  3. Loginova LG, Egorova LA, Golovacheva RS, Seregina LM. Thermus ruber sp. nov., nom. rev. Int J Syst Bacteriol 1984; 34:498–499 [View Article]
    [Google Scholar]
  4. Tenreiro S, Nobre MF, da Costa MS. Thermus silvanus sp. nov. and Thermus chliarophilus sp. nov., two new species related to Thermus ruber but with lower growth temperatures. Int J Syst Bacteriol 1995; 45:633–639 [View Article][PubMed]
    [Google Scholar]
  5. Chen MY, Lin GH, Lin YT, Tsay SS. Meiothermus taiwanensis sp. nov., a novel filamentous, thermophilic species isolated in Taiwan. Int J Syst Evol Microbiol 2002; 52:1647–1654 [View Article][PubMed]
    [Google Scholar]
  6. Pires AL, Albuquerque L, Tiago I, Nobre MF, Empadinhas N et al. Meiothermus timidus sp. nov., a new slightly thermophilic yellow-pigmented species. FEMS Microbiol Lett 2005; 245:39–45 [View Article][PubMed]
    [Google Scholar]
  7. Albuquerque L, Ferreira C, Tomaz D, Tiago I, Veríssimo A et al. Meiothermus rufus sp. nov., a new slightly thermophilic red-pigmented species and emended description of the genus Meiothermus. Syst Appl Microbiol 2009; 32:306–313 [View Article][PubMed]
    [Google Scholar]
  8. Albuquerque L, Rainey FA, Nobre MF, da Costa MS. Meiothermus granaticius sp. nov., a new slightly thermophilic red-pigmented species from the Azores. Syst Appl Microbiol 2010; 33:243–246 [View Article][PubMed]
    [Google Scholar]
  9. Zhang XQ, Zhang WJ, Wei BP, Xu XW, Zhu XF et al. Meiothermus cateniformans sp. nov., a slightly thermophilic species from north-eastern China. Int J Syst Evol Microbiol 2010; 60:840–844 [View Article][PubMed]
    [Google Scholar]
  10. Mori K, Iino T, Ishibashi J, Kimura H, Hamada M et al. Meiothermus hypogaeus sp. nov., a moderately thermophilic bacterium isolated from a hot spring. Int J Syst Evol Microbiol 2012; 62:112–117 [View Article][PubMed]
    [Google Scholar]
  11. Yu TT, Yin YR, Zhang YG, Yao JC, Klenk HP et al. Meiothermus terrae sp. nov., isolated from a geothermally heated soil sample. Int J Syst Evol Microbiol 2014; 64:794–798 [CrossRef]
    [Google Scholar]
  12. Ming H, Duan YY, Guo QQ, Yin YR, Zhou EM et al. Meiothermus roseus sp. nov., a thermophilic bacterium isolated from a geothermal area. Antonie van Leeuwenhoek 2015; 108:897–905 [View Article][PubMed]
    [Google Scholar]
  13. Nobre MF. Genus II. Meiothermus. In Boone DR, Castenholz RW, Garrity GM. (editors) Meiothermus in Bergey’s Manual of Systematic Bacteriology, 2nd ed. vol. 1 New York: Springer; 2001 pp. 414–420
    [Google Scholar]
  14. Cerny G. Studies on the aminopeptidase test for the distinction of gram-negative from gram-positive bacteria. Eur J Appl Microbiol 1978; 5:113–122 [View Article]
    [Google Scholar]
  15. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ et al. Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China. Int J Syst Evol Microbiol 2005; 55:1149–1153 [View Article]
    [Google Scholar]
  16. Degryse E, Glansdorff N, Piérard A. A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus. Arch Microbiol 1978; 117:189–196 [View Article][PubMed]
    [Google Scholar]
  17. Nie GX, Ming H, Li S, Zhou EM, Cheng J et al. Geodermatophilus nigrescens sp. nov., isolated from a dry-hot valley. Antonie van Leeuwenhoek 2012; 101:811–817 [View Article][PubMed]
    [Google Scholar]
  18. Kovacs N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 1956; 178:703 [View Article][PubMed]
    [Google Scholar]
  19. MacFaddin JF. Biochemical Tests for Identification of Medical Bacteria Williams & Wilkins Co; 1976
    [Google Scholar]
  20. Gonzalez C, Gutierrez C, Ramirez C. Halobacterium vallismortis sp. nov. an amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 1978; 24:710–715 [View Article][PubMed]
    [Google Scholar]
  21. Minnikin DE, Collins MD, Goodfellow M. Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Microbiol 1979; 47:87–95
    [Google Scholar]
  22. Collins MD, Jones D. Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. J Appl Microbiol 1980; 48:459–470 [View Article]
    [Google Scholar]
  23. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  24. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article][PubMed]
    [Google Scholar]
  25. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded Ion exchanger as stationary phases. J Liq Chromatogr 1982; 5:2359–2367 [View Article]
    [Google Scholar]
  26. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
    [Google Scholar]
  27. Cui XL, Mao PH, Zeng M, Li WJ, Zhang LP et al. Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 2001; 51:357–363 [View Article][PubMed]
    [Google Scholar]
  28. Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R et al. Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 2007; 57:1424–1428 [View Article][PubMed]
    [Google Scholar]
  29. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
    [Google Scholar]
  30. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  31. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article]
    [Google Scholar]
  32. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
    [Google Scholar]
  33. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  34. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
    [Google Scholar]
  35. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
    [Google Scholar]
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