1887

Abstract

Two Gram-negative, heterotrophic, aerobic, prosthecated, marine bacteria, designated strains MCS23 and MCS27, were isolated from seawater samples. NaCl was required for growth. The major polar lipid detected in strain MCS27 was phosphatidylglycerol, whereas those detected in MCS23 were phosphatidylglycerol, sulfoquinovosyl diacylglycerol and 1,2-diacyl-3-α--glucuronopyranosyl-sn-glycerol taurineamide. The most abundant cellular fatty acids were Cω7 and C, hydroxyl-fatty acids were 3-OH C in both strains and 3-OH C in MCS23. Strains MCS23 and MCS27 had DNA G+C contents of 57.0 and 55.0 mol%, respectively. The two strains shared 99.3 % 16S rRNA gene sequence similarity; levels of similarity with the type strains of species of the genus were 99.4–97.8 % but DNA–DNA hybridizations were 53 % or lower. Besides their 16S rRNA gene sequences, the novel strains can be differentiated from other species of the genus by cell morphology, lipid and fatty acid patterns and enzyme activities. The data obtained led to the identification of two novel species, for which the names sp. nov. (type strain MCS23=LMG 28705=CCUG 66934) and sp. nov. (type strain MCS27=LMG 29152=CCUG 67844) are proposed. As these two novel species are the first prosthecate species in the genus , an emended genus description is also provided.

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2017-08-01
2019-12-14
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