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Abstract

A bacterial strain, designated DL5-4, was isolated from the surface seawater of Dalian Bay and characterized using a polyphasic taxonomy approach. Cells of DL5-4 were Gram-staining-negative, non-motile and short-rod-shaped. Growth was observed at 8–40 °C (optimum 28–30 °C), at pH 6–9 (optimum pH 7) and in 0–7 % NaCl (optimum 1–3 %, w/v). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that DL5-4 formed an independent branch with members of the genus Defluviimonas , sharing high similarities with five related type strains, Defluviimonas aquaemixtae CDM-7 (96.6 %), Defluviimonas denitrificans DSM 18921 (96.0 %), Defluviimonas indica 20V17 (95.8 %), Defluviimonas aestuarii BS14 (95.8 %) and Defluviimonas alba cai42 (94.5 %). The predominant fatty acid was summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c). The isoprenoid quinone was identified as Q-10. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, four phospholipids, an aminolipid and an unknown lipid. The DNA G+C content was 63.8 mol%. The results of the phenotypic, phylogenetic and chemotaxonomic analyses clearly indicated that DL5-4 represents a novel species of the genus Defluviimonas , for which the name Defluviimonas nitratireducens sp. nov. is proposed, with type strain DL5-4 (=MCCC 1A06955=LMG 29616).

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2017-08-15
2019-10-21
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References

  1. Wang W, Zhang R, Shan D, Shao Z. Indigenous oil-degrading bacteria in crude oil-contaminated seawater of the Yellow sea, China. Appl Microbiol Biotechnol 2014;98:7253–7269 [CrossRef][PubMed]
    [Google Scholar]
  2. Foesel BU, Drake HL, Schramm A. Defluviimonas denitrificans gen. nov., sp. nov., and Pararhodobacter aggregans gen. nov., sp. nov., non-phototrophic Rhodobacteraceae from the biofilter of a marine aquaculture. Syst Appl Microbiol 2011;34:498–502 [CrossRef][PubMed]
    [Google Scholar]
  3. Math RK, Jin HM, Jeong SH, Jeon CO. Defluviimonas aestuarii sp. nov., a marine bacterium isolated from a tidal flat, and emended description of the genus Defluviimonas Foesel et al. 2011. Int J Syst Evol Microbiol 2013;63:2895–2900 [CrossRef][PubMed]
    [Google Scholar]
  4. Jiang L, Xu H, Shao Z, Long M. Defluviimonas indica sp. nov., a marine bacterium isolated from a deep-sea hydrothermal vent environment. Int J Syst Evol Microbiol 2014;64:2084–2088 [CrossRef][PubMed]
    [Google Scholar]
  5. Jung YT, Park S, Lee JS, Yoon JH. Defluviimonas aquaemixtae sp. nov., isolated from the junction between a freshwater spring and the ocean. Int J Syst Evol Microbiol 2014;64:4191–4197 [CrossRef][PubMed]
    [Google Scholar]
  6. Pan XC, Geng S, Lv XL, Mei R, Jiangyang JH et al. Defluviimonas alba sp. nov., isolated from an oilfield. Int J Syst Evol Microbiol 2015;65:1805–1811 [CrossRef][PubMed]
    [Google Scholar]
  7. Collee JG, Miles R, Watt B. Tests for identification of bacteria. Mackie and McCartney Practical Medical Microbiology 1996;14:131–149
    [Google Scholar]
  8. Lai Q, Liu Y, Shao Z. Bacillus xiamenensis sp. nov., isolated from intestinal tract contents of a flathead mullet (Mugil cephalus). Antonie van Leeuwenhoek 2014;105:99–107 [CrossRef][PubMed]
    [Google Scholar]
  9. Shieh WY, Chen YW, Chaw SM, Chiu HH. Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. Int J Syst Evol Microbiol 2003;53:479–484 [CrossRef][PubMed]
    [Google Scholar]
  10. Lai Q, Liu Y, Yuan J, du J, Wang L et al. Multilocus sequence analysis for assessment of phylogenetic diversity and biogeography in Thalassospira bacteria from diverse marine environments. PLoS One 2014;9:e106353 [CrossRef][PubMed]
    [Google Scholar]
  11. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  12. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  13. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  14. Rzhetsky A, Nei M. Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. J Mol Evol 1992;35:367–375 [CrossRef][PubMed]
    [Google Scholar]
  15. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  16. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  17. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101 Newark, DE: MIDI; 1990
    [Google Scholar]
  18. Collins M. Isoprenoid quinone analyses in bacterial classification and identification. Society for Applied Bacteriology Technical Series 1985;267–287
    [Google Scholar]
  19. Kates M. Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids Amsterdam: North. Holland Publishing Company; 1986; pp.106–107 241–246
    [Google Scholar]
  20. Mandel M, Igambi L, Bergendahl J, Dodson ML, Scheltgen E. Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. J Bacteriol 1970;101:333–338[PubMed]
    [Google Scholar]
  21. Jiang L, Long M, Shao Z. Draft genome sequence of Defluviimonas indica strain 20V17T, isolated from a deep-sea hydrothermal vent environment in the southwest Indian Ocean. Genome Announc 2014;2:e00479-14 [CrossRef][PubMed]
    [Google Scholar]
  22. Zhao JY, Geng S, Xu L, Hu B, Sun JQ et al. Complete genome sequence of Defluviimonas alba cai42T, a microbial exopolysaccharides producer. J Biotechnol 2016;239:9–12 [CrossRef][PubMed]
    [Google Scholar]
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