1887

Abstract

A Gram-stain-negative, short rod-shaped and non-flagellated bacterium, named strain XMTR2A4, was isolated from the gut of a marine limpet, Notoacmea schrenckii on intertidal rocks. Colonies were small, light grey and circular. Catalase- and oxidase-positive. Growth was observed at 15 to 37 °C (optimum 28–30 °C), with salinity range from 0.5 to 9 % (optimum 1–2 %), and at pH 6 to 9 (optimum pH 7). The almost full-length 16S rRNA gene of strain XMTR2A4 had the highest sequence similarity of 93.7 % with Mycoplana ramosa DSM 7292, and of 93.5 , 93.5 and 93.4 % with Chelativorans intermedius CC-MHSW-5, Ensifer fredii ATCC 35423 and Phyllobacterium myrsinacearum IAM 13584, respectively. Phylogenetic analysis showed that strain XMTR2A4 formed a tight cluster with a cultured but uncharacterized strain, YP382-1-A, which was deeply separated from the species within the order Rhizobiales in the class Alphaproteobacteria . The predominant fatty acid of strain XMTR2A4 was summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 83.4 %). Ubiquinone-10 (Q-10) was detected as the sole respiratory quinone. The polar lipids were identified as phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylmonomethylethanolamine, an unidentified phospholipid and three unknown lipids. The genome size was about 3.6 Mbp and the G+C content was 61.5 mol%. Combining the results above, it was ascertained that strain XMTR2A4represents a novel species of a new genus in the order Rhizobiales , for which the name Notoacmeibacter marinus gen. nov., sp. nov. is proposed. The type strain of the type species is XMTR2A4 (=MCCC 1A01882=KCTC 52427). A novel family in the order Rhizobiales , named Notoacmeibacteraceae fam. nov., is also proposed to accommodate the new genus.

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2017-08-04
2019-12-06
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