1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 8

gen. nov., sp. nov., isolated from the gut of a limpet and proposal of fam. nov. in the order of the class Free

Abstract

A Gram-stain-negative, short rod-shaped and non-flagellated bacterium, named strain XMTR2A4, was isolated from the gut of a marine limpet, on intertidal rocks. Colonies were small, light grey and circular. Catalase- and oxidase-positive. Growth was observed at 15 to 37 °C (optimum 28–30 °C), with salinity range from 0.5 to 9 % (optimum 1–2 %), and at pH 6 to 9 (optimum pH 7). The almost full-length 16S rRNA gene of strain XMTR2A4 had the highest sequence similarity of 93.7 % with DSM 7292, and of 93.5 , 93.5 and 93.4 % with CC-MHSW-5, ATCC 35423 and IAM 13584, respectively. Phylogenetic analysis showed that strain XMTR2A4 formed a tight cluster with a cultured but uncharacterized strain, YP382-1-A, which was deeply separated from the species within the order in the class . The predominant fatty acid of strain XMTR2A4 was summed feature 8 (Cω7 and/or Cω6; 83.4 %). Ubiquinone-10 (Q-10) was detected as the sole respiratory quinone. The polar lipids were identified as phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylmonomethylethanolamine, an unidentified phospholipid and three unknown lipids. The genome size was about 3.6 Mbp and the G+C content was 61.5 mol%. Combining the results above, it was ascertained that strain XMTR2A4represents a novel species of a new genus in the order , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is XMTR2A4 (=MCCC 1A01882=KCTC 52427). A novel family in the order , named fam. nov., is also proposed to accommodate the new genus.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): limpet , Notoacmea schrenckii , Notoacmeibacter marinus and Rhizobiales
© 2017 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001951
2017-08-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/8/2527.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001951&mimeType=html&fmt=ahah

References

  1. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
     [Google Scholar]
  2. Tanaka T, Kawasaki K, Daimon S, Kitagawa W, Yamamoto K et al. A hidden pitfall in the preparation of agar media undermines microorganism cultivability. Appl Environ Microbiol 2014; 80:7659–7666 [View Article][PubMed]
     [Google Scholar]
  3. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  4. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012; 19:455–477 [View Article][PubMed]
     [Google Scholar]
  5. Gurevich A, Saveliev V, Vyahhi N, Tesler G. QUAST: quality assessment tool for genome assemblies. Bioinformatics 2013; 29:1072–1075 [View Article][PubMed]
     [Google Scholar]
  6. Hyatt D, Chen GL, Locascio PF, Land ML, Larimer FW et al. Prodigal: prokaryotic gene recognition and translation initiation site identification. BMC Bioinformatics 2010; 11:119 [View Article][PubMed]
     [Google Scholar]
  7. Zuo G, Hao B. CVTree3 web server for whole-genome-based and alignment-free prokaryotic phylogeny and taxonomy. Genomics Proteomics Bioinformatics 2015; 13:321–331 [View Article][PubMed]
     [Google Scholar]
  8. Ostle AG, Holt JG. Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate. Appl Environ Microbiol 1982; 44:238–241[PubMed]
     [Google Scholar]
  9. Urakami T, Oyanagi H, Araki H, Suzuki K-I, Komagata K. Recharacterization and emended description of the Genus Mycoplana and description of two new species, Mycoplana ramosa and Mycoplana segnis. Int J Syst Bacteriol 1990; 40:434–442 [View Article]
     [Google Scholar]
  10. Scholla MH, Elkan GH. Rhizobium fredii sp. nov., a fast-growing species that effectively nodulates soybeans. Int J Syst Bacteriol 1984; 34:484–486 [View Article]
     [Google Scholar]
  11. Mergaert J, Cnockaert MC, Swings J. Phyllobacterium myrsinacearum (subjective synonym Phyllobacterium rubiacearum) emend. Int J Syst Evol Microbiol 2002; 52:1821–1823 [View Article][PubMed]
     [Google Scholar]
  12. Peix A, Rivas R, Trujillo ME, Vancanneyt M, Velázquez E et al. Reclassification of Agrobacterium ferrugineum LMG 128 as Hoeflea marina gen. nov., sp. nov. Int J Syst Evol Microbiol 2005; 55:1163–1166 [View Article][PubMed]
     [Google Scholar]
  13. Lai Q, Yu Z, Yuan J, Sun F, Shao Z. Nitratireductor indicus sp. nov., isolated from deep-sea water. Int J Syst Evol Microbiol 2011; 61:295–298 [View Article][PubMed]
     [Google Scholar]
  14. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–207 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001951
Loading
Notoacmeibacter marinus gen. nov., sp. nov., isolated from the gut of a limpet and proposal of Notoacmeibacteraceae fam. nov. in the order Rhizobiales of the class Alphaproteobacteria
Int J Syst Evol Microbiol 67, 2527 (2017); https://doi.org/10.1099/ijsem.0.001951
/content/journal/ijsem/10.1099/ijsem.0.001951
/content/journal/ijsem/10.1099/ijsem.0.001951
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error