1887

Abstract

A Gram-stain-negative and rod-shaped bacterial strain, 12-OD1, with rock phosphate solubilizing ability was isolated from agricultural soil in Hailun, Heilongjiang, PR China. The isolate was affiliated to the genus Massilia , based on 16S rRNA gene sequence alignments, having the highest similarities with Massilia putida 6 NM-7 (98.67 %), Massilia kyonggiensis TSA1 (98.28 %), and Massilia norwichensis NS9 (98.07 %), respectively. The DNA G+C content was 67.72 mol% and DNA–DNA hybridization showed low relatedness values (less than 47 %) between strain 12-OD1 and other phylogenetically related species of the genus Massilia . The predominant isoprenoid quinone was Q-8 and the polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were C17 : 0 cyclo (25.4 %), C16 : 0 (23.4 %) and summed feature 3 (C16 : 1ω7c and/or C16 : 1 ω6c) (22.5 %), which differentiates it from close relatives within the genus Massilia . Combined genetic, physiological and biochemical properties indicate that strain 12-OD1 is a novel species of the genus Massilia , for which the name Massilia phosphatilytica sp. nov., is proposed, with the type strain 12-OD1 (=CCTCC AB 2016251=LMG 29956=KCTC 52513).

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2017-08-08
2019-10-22
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