1887

Abstract

A novel acid-tolerant and alkalitolerant gammaproteobacterium designated strain RS22 was isolated from Kyonggi University forest soil. Cells were aerobic, Gram-stain-negative, catalase- and oxidase-positive, non-motile, non-spore-forming, rod-shaped and yellow-pigmented. Flexirubin-type pigments were absent. Strain RS22 was able to assimilate lactic acid, -proline and 3-hydroxybenzoic acid; it tolerated 4 % (w/v) NaCl, fermented glucose and was able to grow at pH 11.0. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain RS22 formed a lineage within the class of the phylum that was distinct from various species of the genus , including 2APBS1 (97.7 % sequence similarity), LCS2 (97.5 %), GP18-1 (97.5 %) and DCY45 (97.2 %). The predominant respiratory quinone was Q-8. The major polar lipids of strain RS22 were phosphatidylethanolamine, phosphatidyl--methylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major cellular fatty acids were summed feature 9 (iso-Cω9 and/or C 10-methyl), iso-C, iso-C, iso-C, anteiso-C and C. The DNA G+C content of strain RS22 was 63.2 mol%. DNA–DNA hybridization between strain RS22 and other closest members of the genus revealed relatedness values from 28 to 51 %. On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, strain RS22 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RS22 (=KEMB 9005-480=KACC 19048=NBRC 112473).

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2017-05-01
2019-12-15
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