@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001786, author = "Dahal, Ram Hari and Kim, Jaisoo", title = "Rhodanobacter humi sp. nov., an acid-tolerant and alkalitolerant gammaproteobacterium isolated from forest soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "5", pages = "1185-1190", doi = "https://doi.org/10.1099/ijsem.0.001786", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001786", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "alkalitolerant", keywords = "forest soil", keywords = "Rhodanobacter humi sp. nov", keywords = "Gammaproteobacteria", keywords = "acid-tolerant", abstract = "A novel acid-tolerant and alkalitolerant gammaproteobacterium designated strain RS22T was isolated from Kyonggi University forest soil. Cells were aerobic, Gram-stain-negative, catalase- and oxidase-positive, non-motile, non-spore-forming, rod-shaped and yellow-pigmented. Flexirubin-type pigments were absent. Strain RS22T was able to assimilate lactic acid, l-proline and 3-hydroxybenzoic acid; it tolerated 4 % (w/v) NaCl, fermented glucose and was able to grow at pH 11.0. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain RS22T formed a lineage within the class Gammaproteobacteria of the phylum Proteobacteria that was distinct from various species of the genus Rhodanobacter , including Rhodanobacter denitrificans 2APBS1T (97.7 % sequence similarity), Rhodanobacter thiooxydans LCS2T (97.5 %), Rhodanobacter terrae GP18-1T (97.5 %) and Rhodanobacter soli DCY45T (97.2 %). The predominant respiratory quinone was Q-8. The major polar lipids of strain RS22T were phosphatidylethanolamine, phosphatidyl-N-methylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major cellular fatty acids were summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl), iso-C15 : 0, iso-C17 : 0, iso-C16 : 0, anteiso-C15 : 0 and C16 : 0. The DNA G+C content of strain RS22T was 63.2 mol%. DNA–DNA hybridization between strain RS22T and other closest members of the genus Rhodanobacter revealed relatedness values from 28 to 51 %. On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, strain RS22T represents a novel species of the genus Rhodanobacter , for which the name Rhodanobacter humi sp. nov. is proposed. The type strain is RS22T (=KEMB 9005-480T=KACC 19048T=NBRC 112473T).", }