1887

Abstract

A Gram-stain-positive, aerobic, endospore-forming bacterial strain isolated from the rhizosphere of was studied to determine its detailed taxonomic position. Based on 16S rRNA gene sequence similarity comparisons, strain JJ-64 was shown to be a member of the genus , most closely related to the type strains of (99 %) and (97.5 %). 16S rRNA gene sequence similarity to all other species was ≤97.5 %. DNA–DNA hybridization values to the type strains of and were 51 % (reciprocal 25 %) and 31 % (reciprocal 37 %), respectively. The presence of diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, the major quinone MK-7 and the polyamine pattern with spermidine as the major component were well in line with the characteristics of the genus . Furthermore, the polar lipid profile of strain JJ-64 with the predominant lipids diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and two unidentified aminophospholipids reflected the close phylogenetic relatedness to . Major fatty acids were iso- and anteiso-branched components. Physiological and biochemical characteristics allowed the further phenotypic differentiation of strain JJ-64 from the most closely related species. Thus, strain JJ-64 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JJ-64 (=LMG 29875=CCM 8725).

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2017-04-01
2024-11-09
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