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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 4

sp. nov., an obligately anaerobic, dissimilatory antimonate- and arsenate-reducing bacterium isolated from anoxic sediments, and emended description of the genus Free

Abstract

A novel anaerobic, Gram-stain-negative, endospore-forming bacterium, designated strain MLFW-2, was isolated from anoxic sediments collected from the drainage area of a geothermal spring near Mono Lake, CA, USA. Optimal growth was achieved at 34 °C and pH 8.25–8.50 in medium containing 0.75 % (w/v) NaCl. Catalase, but not oxidase, was produced. Strain MLFW-2 was an obligate anaerobe capable of respiring with nitrate, nitrite, DMSO, arsenate, antimonate, selenate and selenite as terminal electron acceptors. Lactate, pyruvate, formate and H could serve as electron donors to support growth. The isolate was incapable of fermentation. The predominant fatty acids were C, Cω9, Cω7, Cω9 and Cω7. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The only isoprenoid quinone detected was menaquinone 7 (MK-7). The DNA G+C content was 38.2 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence demonstrated that strain MLFW-2 was a member of the order and was most closely related to AHT28 (93.9 % similarity). On the basis of phenotypic and phylogenomic evidence, strain MLFW-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MLFW-2 (=DSM 28709=JCM 30866). An emended description of the genus is also provided.

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Keyword(s): anaerobic respiration , antimony , arsenic and Mono Lake
© 2017 IUMS
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2017-04-01
2024-05-15
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Desulfuribacillus stibiiarsenatis sp. nov., an obligately anaerobic, dissimilatory antimonate- and arsenate-reducing bacterium isolated from anoxic sediments, and emended description of the genus Desulfuribacillus
Int J Syst Evol Microbiol 67, 1011 (2017); https://doi.org/10.1099/ijsem.0.001732
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