1887

Abstract

A bacterial strain designated GTAE24 was isolated from a root of wheat growing in soil from the Canary Islands, Spain. Phylogenetic analyses based on 16S rRNA gene sequences placed the isolate in the genus Brevundimonas with Brevundimonas abyssalis TAR-001 as its closest relative at 99.4 % similarity. DNA–DNA hybridization studies showed an average of 38 % relatedness between strain GTAE24 and the type strain of B. abyssalis . Cells were Gram-stain-negative and motile by polar flagella. The strain was positive for oxidase and weakly positive for catalase. Gelatin, starch and casein were not hydrolysed. Growth was supported by many carbohydrates and organic acids as carbon source. Ubiquinone Q-10 was the predominant isoprenoid quinone and C18 : 1ω7c/C18  : 1ω6c (summed feature 8) and C16 : 0 were the major fatty acids. The major polar lipids were phosphatidylglycerol, 1,2-di-O -acyl-3-O-[d-glucopyranosyl-(1,4)-α-d-glucopyranuronosyl] glycerol, 1,2-diacyl-3-O-[6′-phosphatidyl-α-d-glucopyranosyl] glycerol, 1,2-di-O-acyl-3-O-α-d-glucopyranosyl glycerol, and 1,2-di-O-acyl-3-O-α-d-glucopyranuronosyl glycerol. The DNA G+C content was 63.9 mol%. Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain GTAE24 should be considered as representing a novel species of the genus Brevundimonas , for which the name Brevundimonas canariensis sp. nov. is proposed. The type strain is GTAE24 (=LMG 29500=CECT 9126).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001725
2017-05-05
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/4/969.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001725&mimeType=html&fmt=ahah

References

  1. Vancanneyt M, Segers P, Abraham WR, Vos PD.Brevundimonas. In Bergey's Manual of Systematics of Archaea and Bacteria NJ: John Wiley & Sons, Inc., in association with Bergey's Manual Trust; 2015; pp.1–14
    [Google Scholar]
  2. Doetsch RN. Determinative methods of light microscopy. In Gerdhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA. et al (editors) Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology; 1981; pp.21–33
    [Google Scholar]
  3. Rivas R, García-Fraile P, Mateos PF, Martínez-Molina E, Velázquez E. Characterization of xylanolytic bacteria present in the bract phyllosphere of the date palm Phoenix dactylifera. Lett Appl Microbiol 2007;44:181–187 [CrossRef][PubMed]
    [Google Scholar]
  4. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990;215:403–410 [CrossRef][PubMed]
    [Google Scholar]
  5. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  6. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res 1997;25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  7. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  8. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  9. Rogers JS, Swofford DL. A fast method for approximating maximum likelihoods of phylogenetic trees from nucleotide sequences. Syst Biol 1998;47:77–89[PubMed][CrossRef]
    [Google Scholar]
  10. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  11. Chun J, Goodfellow M. A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 1995;45:240–245 [CrossRef][PubMed]
    [Google Scholar]
  12. Mandel M, Marmur J. Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 1968;12:195–206[CrossRef]
    [Google Scholar]
  13. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989;39:224–229 [CrossRef]
    [Google Scholar]
  14. Willems A, Doignon-Bourcier F, Goris J, Coopman R, De Lajudie P et al. DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 2001;51:1315–1322 [CrossRef][PubMed]
    [Google Scholar]
  15. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987;37:463–464 [CrossRef]
    [Google Scholar]
  16. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  17. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990;66:199–202 [CrossRef]
    [Google Scholar]
  18. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  19. Kroppenstedt RM, Goodfellow M. The family thermomonosporaceae: Actinocorallia, Actinomadura, Spirillispora and Thermomonospora. In Dworkin M, Falkow S, Schleifer KH, Stackebrandt E. (editors) The Prokaryotes. Archaea and Bacteria: Firmicutes, Actinomycetes, 3rd ed.vol. 3 New York: Springer; 2006; pp.682–724
    [Google Scholar]
  20. Tsubouchi T, Shimane Y, Usui K, Shimamura S, Mori K et al. Brevundimonas abyssalis sp. nov., a dimorphic prosthecate bacterium isolated from deep-subsea floor sediment. Int J Syst Evol Microbiol 2013;63:1987–1994 [CrossRef][PubMed]
    [Google Scholar]
  21. Segers P, Vancanneyt M, Pot B, Torck U, Hoste B et al. Classification of Pseudomonas diminuta Leifson and Hugh 1954 and Pseudomonas vesicularis Büsing, Döll, and Freytag 1953 in Brevundimonas gen. nov. as Brevundimonas diminuta comb. nov. and Brevundimonas vesicularis comb. nov., respectively. Int J Syst Bacteriol 1994;44:499–510 [CrossRef][PubMed]
    [Google Scholar]
  22. Abraham WR, Strömpl C, Meyer H, Lindholst S, Moore ER et al. Phylogeny and polyphasic taxonomy of Caulobacter species. Proposal of Maricaulis gen. nov. with Maricaulis maris (Poindexter) comb. nov. as the type species, and emended description of the genera Brevundimonas and Caulobacter. Int J Syst Bacteriol 1999;49:1053–1073 [CrossRef][PubMed]
    [Google Scholar]
  23. Lemberg R, Foulkes EC. Reaction between catalase and hydrogen peroxide. Nature 1948;161:131–132 [CrossRef][PubMed]
    [Google Scholar]
  24. Kovacs N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 1956;178:703 [CrossRef][PubMed]
    [Google Scholar]
  25. Claus D, Berkeley RCW. Genus Bacillus Cohn 1872, 174AL. In Sneath PHA, Mair NS, Sharpe ME, Holt JG. (editors) Bergey’s Manual of Systematic Bacteriologyvol. 2 Baltimore: Williams & Wilkins; 1986; pp.1105–1139
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001725
Loading
/content/journal/ijsem/10.1099/ijsem.0.001725
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error