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Abstract

A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterial strain, designated ISSFR-015, was isolated from a high-efficiency particulate arrestance filter in the International Space Station and was characterized by polyphasic taxonomy. A comparative analysis of the 16S rRNA gene sequence (1494 bp) of strain ISSFR-015 showed highest similarity to Solibacillus isronensis B3W22 (98.9 %), followed by Solibacillus silvestris HR3-23 (98.6 %) and Bacillus cecembensis PN5 (96.7 %). DNA–DNA hybridization analysis revealed that the DNA relatedness values of strain ISSFR-015 with other closely related species were in the range of 41–47 % [ S. silvestris MTCC 10789 (47 %), S. isronensis MTCC 7902 (41 %) and B. cecembensis MTCC 9127 (43 %)]. The DNA G+C content of strain ISSFR-015 was 45.4 mol%. The major fatty acids were iso-C15 : 0 (45.2 %) and C17 : 1ω10c (12.1 %). The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and one unknown phospholipid. The isoprenoid quinones present in strain ISSFR-015 were MK-7 (86.8 %), MK-6 (11.6 %) and MK-8 (1.0 %). The peptidoglycan type of the cell wall was A4α l-Lys-d-Glu. Based on the phylogenetic analysis, strain ISSFR-015 belongs to the genus Solibacillus . The polyphasic taxonomic data, including low DNA–DNA hybridization values, and the chemotaxonomic analysis confirmed that strain ISSFR-015 represents a novel species, for which the name Solibacillus kalamii sp. nov. is proposed. The type strain for this proposed species is ISSFR-015 (=NRRL B-65388=DSM 101595).

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2017-05-05
2019-10-17
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