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Abstract

A novel, Gram-stain-negative, aerobic, non-motile and filamentous bacterial strain, designated HMF3829, was isolated from wetland freshwater in Gyeong-an wetland, Republic of Korea. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HMF3829 formed a lineage within the genus Runella . Strain HMF3829 was closely related to Runella slithyformis DSM 19594 (95.2 % 16S rRNA gene sequence similarity), Runella limosa DSM 17973 (94.9 %), Runella zeae NS12 (94.2 %) and Runella defluvii EMB13 (94.0 %). The major fatty acids of strain HMF3829 were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C16 : 1ω5c and iso-C17 : 0 3-OH. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The polar lipids of strain HMF3829 consisted of phosphatidylethanolamine, three unidentified aminolipids, one unidentified phospholipid, three unidentified aminophospholipids and 12 unidentified lipids. The DNA G+C content of strain HMF3829 was 46.2 mol%. On the basis of the evidence presented in this study, strain HMF3829 represents a novel species of the genus Runella , for which the name Runella palustris sp. nov. is proposed. The type strain is HMF3829 (=KCTC 42850=CECT 8978).

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/content/journal/ijsem/10.1099/ijsem.0.001692
2017-04-03
2019-10-15
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References

  1. Stanier RY. Studies on the Cytophagas. J Bacteriol 1940;40:619–635[PubMed]
    [Google Scholar]
  2. Larkin JM, Williams PM. Runella slithyformis gen. nov., sp. nov., a curved, nonflexible, pink bacterium. Int J Syst Bacteriol 1978;28:32–36[CrossRef]
    [Google Scholar]
  3. Lu S, Lee JR, Ryu SH, Chung BS, Choe WS et al. Runella defluvii sp. nov., isolated from a domestic wastewater treatment plant. Int J Syst Evol Microbiol 2007;57:2600–2603 [CrossRef][PubMed]
    [Google Scholar]
  4. Ryu SH, Nguyen TT, Park W, Kim CJ, Jeon CO. Runella limosa sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 2006;56:2757–2760 [CrossRef][PubMed]
    [Google Scholar]
  5. Chelius MK, Henn JA, Triplett EW. Runella zeae sp. nov., a novel gram-negative bacterium from the stems of surface-sterilized Zea mays. Int J Syst Evol Microbiol 2002;52:2061–2063 [CrossRef][PubMed]
    [Google Scholar]
  6. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991; pp.125–175
    [Google Scholar]
  7. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  8. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  9. Pruesse E, Peplies J, Glöckner FO. SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 2012;28:1823–1829 [CrossRef][PubMed]
    [Google Scholar]
  10. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  11. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376[PubMed][CrossRef]
    [Google Scholar]
  12. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406 [CrossRef]
    [Google Scholar]
  13. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783[CrossRef]
    [Google Scholar]
  14. Stackebrandt E, Goebel B. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994;44:846–849[CrossRef]
    [Google Scholar]
  15. Brown A. Benson’s Microbiological Application Laboratory Manual in General Microbiology New York: McGraw-Hill; 2007
    [Google Scholar]
  16. Sasser M. Identification of Bacteria By Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  17. Minnikin DE, O'Donnell AF, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241[CrossRef]
    [Google Scholar]
  18. Collins M. Analysis of isoprenoid Quinones. Methods Microbiol 1985;18:329–366[CrossRef]
    [Google Scholar]
  19. Gonzalez JM, Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 2002;4:770–773[PubMed][CrossRef]
    [Google Scholar]
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