1887

Abstract

A bacterial strain designated strain SK-11 was isolated from the acidic soil of a deciduous forest in the Shirakami Mountains in Japan. Cells of strain SK-11 were aerobic, non-motile, Gram-stain-negative rods, 0.7–1.0 µm in width and 1.0–1.4 µm in length. The pH range for growth was between pH 4.0 and 5.5, with an optimum at pH 5.0. The temperature range for growth was between 10 and 35 °C, with an optimum at around 25–30 °C. Strain SK-11 utilized various carbohydrates as growth substrates as well as yeast extract and protein hydrolysates. The major cellular fatty acids (>10 % of total fatty acid contents) were iso-C15 : 0 (55.4 %), iso-C17 : 0 (16.7 %) and iso-C17 : 1ω9c/10 methyl-hexadecanoic acid (17.7 %). The major respiratory quinone was MK-8. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids and an unidentified polar lipid. The DNA G+C content of strain SK-11 was 56.9 %. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SK-11 belonged to the family Acidobacteriaceae within subdivision 1 of the phylum Acidobacteria , and the closest relatives of strain SK-11 were Acidicapsa ligni WH120 and Acidicapsa borealis KA1, with 16S rRNA gene sequence similarities of 96.6 and 96.5 %, respectively. On the basis of the evidence from our polyphasic study, we concluded that strain SK-11 represents a novel species of the genus Acidicapsa , and propose the name Acidicapsa acidisoli sp. nov. The type strain of Acidicapsa acidisolisp nov. is SK-11 (=DSM 100508=NBRC 111227).

Keyword(s): Acidobacteria and subdivision I
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2017-05-05
2019-10-17
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