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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 3

sp. nov., a skin bacterium associated with infections in dogs Free

Abstract

Gram-stain-positive cocci were isolated from miscellaneous sites of the skin of healthy dogs as well as from infection sites in dogs. The closest relative by sequencing of the 16S rRNA gene was with 99.7 % sequence identity, but compared with , the novel strains shared only 90.8 to 93.5 % DNA sequence identity with , , and partial genes, respectively. The novel strains also exhibited differential phenotypic characteristics from , and the majority displayed a visible haemolysis on sheep blood agar, while did not have any haemolytic activity. They generated different matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) MS spectral profiles compared with the other species of the genus . Furthermore, strain KM 45013 shared only 53.7 % DNA–DNA relatedness with the type strain of , confirming that they do not belong to the same species. The DNA G+C content of strain KM 45013 was 36.9 mol%. The most abundant fatty acids were C, Cω6 (6, 9, 12) and C n alcohol. MK-6 was the menaquinone type of KM 45013. Cell-wall structure analysis revealed that the peptidoglycan type was A3α -Lys–Gly–-Ser. Based on genotypic and chemotaxonomic characteristics, we propose to classify these strains within a novel species of the genus for which the name sp. nov. is proposed. The type strain is KM 45013 (=DSM 101690=CCOS 969=CCUG 68920).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): animals , cocci , Gram-positive and Staphylococcaceae
© 2017 IUMS
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2017-03-01
2024-04-26
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References

  1. Götz F, Bannerman T, Schleifer KH. The Genera Staphylococcus and Macrococcus. In Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E. et al (editors) The Prokaryotes, Bacteria: Firmicutes, Cyanobacteria, 3rd ed. vol. 4 Springer; 2006 pp. 5–75
     [Google Scholar]
  2. Mannerová S, Pantůcek R, Doskar J, Svec P, Snauwaert C et al. Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov. and Macrococcus lamae sp. nov., from the skin of llamas. Int J Syst Evol Microbiol 2003; 53:1647–1654 [View Article][PubMed]
     [Google Scholar]
  3. Kloos WE, Ballard DN, George CG, Webster JA, Hubner RJ et al. Delimiting the genus Staphylococcus through description of Macrococcus caseolyticus gen. nov., comb. nov. and Macrococcus equipercicus sp. nov., and Macrococcus bovicus sp. nov. and Macrococcus carouselicus sp. nov. Int J Syst Evol Microbiol 1998; 48:859–877 [View Article][PubMed]
     [Google Scholar]
  4. Gómez-Sanz E, Schwendener S, Thomann A, Gobeli Brawand S, Perreten V. First staphylococcal cassette chromosome mec containing a mecB-carrying gene complex independent of transposon Tn6045 in a Macrococcus caseolyticus isolate from a canine infection. Antimicrob Agents Chemother 2015; 59:4577–4583 [View Article][PubMed]
     [Google Scholar]
  5. de La Fuente R, Suarez G, Ruiz Santa Quiteria JA, Meugnier H, Bes M et al. Identification of coagulase negative staphylococci isolated from lambs as Staphylococcus caseolyticus. Comp Immunol Microbiol Infect Dis 1992; 15:47–52 [View Article][PubMed]
     [Google Scholar]
  6. Kuhnert P, Frey J, Lang NP, Mayfield L. Phylogenetic analysis of Prevotella nigrescens, Prevotella intermedia and Porphyromonas gingivalis clinical strains reveals a clear species clustering. Int J Syst Evol Microbiol 2002; 52:1391–1395 [View Article][PubMed]
     [Google Scholar]
  7. Ye Y, Jiang Q, Wu Q, Zhang J, Lu J et al. The characterization and comparison of Staphylococcus aureus by antibiotic susceptibility testing, enterobacterial repetitive intergenic consensus-polymerase chain reaction, and random amplified polymorphic DNA-polymerase chain reaction. Foodborne Pathog Dis 2012; 9:168–171 [View Article][PubMed]
     [Google Scholar]
  8. Riesen A, Perreten V. Staphylococcus rostri sp. nov., a haemolytic bacterium isolated from the noses of healthy pigs. Int J Syst Evol Microbiol 2010; 60:2042–2047 [View Article][PubMed]
     [Google Scholar]
  9. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Evol Microbiol 1989; 39:159–167 [View Article]
     [Google Scholar]
  10. Miller LT. A single derivatization method for bacterial fatty acid methyl esters including hydroxy acids. J Clin Microbiol 1982; 16:584–586
     [Google Scholar]
  11. Kuykendall LD, Roy MA, O'Neill JJ, Devine TE. Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Evol Microbiol 1988; 38:358–361 [View Article]
     [Google Scholar]
  12. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Med Sci 1959; 37:911–917
     [Google Scholar]
  13. Tindall BJ, Sikorski J, Smibert RM, Kreig NR. Phenotypic characterization and the principe of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM. et al (editors) Methods for General and Molecular Microbiology Washington, DC: ASM Press; 2007 pp. 330–393
     [Google Scholar]
  14. Tindall BJ. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990; 13:128–130 [View Article]
     [Google Scholar]
  15. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990; 66:199–202 [View Article]
     [Google Scholar]
  16. Schumann P. Peptidoglycan structure. In Rainey F, Oren A. (editors) Taxonomy of Prokaryotes, Methods in Microbiology vol. 38 London: Academic Press; 2011 pp. 101–129 [CrossRef]
     [Google Scholar]
  17. Cashion P, Holder-Franklin MA, McCully J, Franklin M. A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 1977; 81:461–466 [View Article][PubMed]
     [Google Scholar]
  18. De Ley J, Cattoir H, Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 1970; 12:133–142 [View Article][PubMed]
     [Google Scholar]
  19. Huss VAR, Festl H, Schleifer KH. Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 1983; 4:184–192 [View Article]
     [Google Scholar]
  20. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987; 37:463–464 [View Article]
     [Google Scholar]
  21. Freney J, Kloos WE, Hajek V, Webster JA, Bes M et al. Recommended minimal standards for description of new staphylococcal species. Int J Syst Bacteriol 1999; 49:489–502 [View Article]
     [Google Scholar]
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Macrococcus canis sp. nov., a skin bacterium associated with infections in dogs
Int J Syst Evol Microbiol 67, 621 (2017); https://doi.org/10.1099/ijsem.0.001673
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