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Abstract

A strictly anaerobic Gram-stain-negative bacterium, designated strain SEBR 4223, was isolated from the production water of an offshore Congolese oil field. Cells were non-motile, pleomorphic and had spherical, annular or budding shapes, often exhibiting long stalks. Strain SEBR 4223 grew on a range of carbohydrates, optimally at 37 °C and pH 7, in a medium containing 40 g l NaCl. Predominant fatty acids were C14 : 0, C14 : 0 DMA, C16 : 0 and C16 : 1ω7c and the major polar lipids were phosphoglycolipids, phospholipids, glycolipids and diphosphatidylglycerol. The G+C content of the DNA was 28.7 mol%. Phylogenetic analysis, based on the 16S rRNA gene sequence, showed that strain SEBR 4223 and Sphaerochaeta multiformis MO-SPC2 formed a cluster with similarity to other species of the genus Sphaerochaeta of of less than 86 %. On the basis of the phenotypic characteristics and taxonomic analyses, we propose a novel genus, Pleomorphochaeta gen. nov., to accommodate the novel species Pleomorphochaeta caudata sp. nov., with SEBR 4223 (=DSM 103077=JCM 31 475) as the type strain. We also propose the reclassification of Sphaerochaeta multiformis MO SPC2 as Pleomorphochaeta multiformis MO-SPC2 comb. nov., the type strain of this novel genus and emend description of the genus Sphaerochaeta .

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2017-02-24
2019-09-22
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