- Volume 67, Issue 2, 2017

A Gram-stain-positive, strictly aerobic, mesophilic bacterium, designated H004T, was isolated from a water sample of the hypersaline and heliothermal Lake Ursu, Sovata, Romania. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H004T formed a distinct phyletic lineage within the genus Rhodococcus . It shared the highest 16S rRNA gene sequence similarity with Rhodococcus yunnanensis YIM 70056T (98.80 %), followed by Rhodococcus fascians LMG 3623T (98.73 %), Rhodococcus cercidiphylli YIM 65003T (98.73 %), Rhodococcus cerastii C5T (98.58 %) and Rhodococcus kyotonensis DS472T (98.53 %). The alkB-based phylogenetic analysis further confirmed that this strain constitutes a highly unique lineage within the genus. Chemotaxonomic characteristics, including the predominant fatty acids acids C15 : 0, C18 : 1ω9c, C19 : 1ω11c/C19  : 1ω9c and C16 : 1ω7c/iso-C15  : 0 2-OH, the major quinone MK-8(H2), the presence of mycolic acids and cell-wall chemotype IV were also consistent with the properties of members of the genus Rhodococcus . The DNA G+C content of strain H004T was 65.4 mol%. The results of DNA–DNA hybridization analyses with the closest relatives, in combination with the alkB-based phylogenetic analysis, as well as the chemotaxonomic and physiological data, demonstrated that isolate H004T represents a novel species of the genus Rhodococcus , for which the name Rhodococcus sovatensissp. nov. is proposed. The type strain is H004T (=DSM 102881T=NCAIM B.02632T).

An endophytic actinobacterium, strain TMS7T, was isolated from the stem of a Thai medicinal plant collected from the grounds of the Phujong-Nayoa National park, Ubon Ratchathani province, Thailand. As a result of a polyphasic taxonomy study, this strain was identified as a member of the genus Micromonospora . This strain was a Gram-stain-positive, aerobic actinobacterium with well-developed substrate mycelium with hyphae forming a single microspore was non-motile. Stran TMS7T was identified according to its 16S rRNA gene sequence as a new member of the genus Micromonospora . The closest phylogenetic members sharing a similarity were Micromonospora chersina DSM 44151T at 99.4 % and Micromonospora rosaria DSM 803T, Micromonospora tulbaghiae TVU1T, Micromonospora inositola DSM 43819T and Micromonospora endolithica DSM 44398T all at 99.2 %. Chemotaxonomic data including cell wall components, major menaquinones and major fatty acids confirmed the affiliation of strain TMS7T to the genus Micromonospora . The results of the phylogenetic analysis, addition to physiological and biochemical studies in combination with DNA–DNA hybridization, allowed the genotypic and phenotypic differentiation of strain TMS7T and the most closely related species with validly published names. The name proposed for the novel species is Micromonospora terminaliae sp. nov. The type strain is TMS7T (=DSM 101760T=NRRL B-65345T).

A novel strain belonging to the genus Promicromonospora , designated HM 533T, was isolated from soil in Kerman Province, Iran. It produced long and branched hyphae on ISP 2 medium that developed into a large number of irregular-shaped spores. It showed optimal growth at 25–30 °C and pH 5.0–8.0 with 0–4 % (w/v) NaCl. The peptidoglycan type of strain HM 533T was A4α l-Lys–l-Ala–d-Glu. Whole-cell hydrolysates of strain HM 533T contained the sugars ribose, glucose and galactose. The main phospholipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, two unknown glycolipids and an unknown phospholipid. MK-9(H4) and MK-9(H2) were the predominant menaquinones. The fatty acids pattern was mainly composed of the saturated branched-chain acids anteiso-C15 : 0 and iso-C15 : 0. The 16S rRNA gene sequence analysis showed the highest pairwise sequence identity (99.5–97.1 %) with the members of the genus Promicromonospora . Based on phenotypic and genotypic features, strain HM 533T is considered to represent a novel species of the genus Promicromonospora , for which the name Promicromonospora kermanensis is proposed with strain HM 533T (=DSM 45485T=UTMC 00533T=CECT 8709T) as the type strain.

A novel endophytic actinomycete with antifungal activity, designated strain NEAU-JXY5T, was isolated from the root of Parathelypteris beddomei (Bak.) Ching. Strain NEAU-JXY5T showed closest 16S rRNA gene sequence similarity to Micromonospora luteifusca GUI2T (99.31 %), and phylogenetically clustered with Micromonospora noduli GUI43T (99.24 %), ' Micromonospora lycii' NEAU-gq11 (99.19 %), ' Micromonospora zeae ' NEAU-gq9 (99.12 %), Micromonospora saelicesensis Lupac 09T (98.97 %), Micromonospora vinacea GUI63T (98.96 %), ' Micromonospora jinlongensis ' NEAU-GRX11 (98.91 %), Micromonospora profundi DS3010T (98.77 %), Micromonospora zamorensis CR38T (98.76 %), Micromonospora chokoriensis 2–19(6)T (98.71 %), Micromonospora lupini Lupac 14NT (98.69 %), Micromonospora ureilytica GUI23T (98.69 %), Micromonospora violae NEAU-zh8T (98.57 %) and Micromonospora taraxaci NEAU-P5T (98.37 %). Phylogenetic analysis based on gyrB gene sequences also indicated that the isolate clustered with the above strains except M. violae NEAU-zh8T. A combination of DNA–DNA hybridization results and some phenotypic characteristics indicated that the strain could be readily distinguished from these closest phylogenetic relatives. Therefore, it is concluded that strain NEAU-JXY5T represents a novel species of the genus Micromonospora , for which the name Micromonospora parathelypteridis sp. nov. is proposed. The type strain is NEAU-JXY5T (=CGMCC 4.7347T=DSM 103125T).

A Gram-stain-positive actinobacterium, designated strain YIM DR4008T, was isolated from the root sample of Psammosilene tunicoides collected from Lijiang, Yunnan, China. Strain YIM DR4008T could grow at temperatures ranging from 10 to 50 °C (optimum 28–30 °C), at pH 5.0–11.0 (optimum pH 7.0) and in the presence of up to 4 % (w/v) NaCl. Sequence analysis of the 16S ribosomal RNA gene revealed that strain YIM DR4008T shared highest similarity (95.0 %) with Streptomyces griseoplanus NBRC 12779T and <95 % similarity with other known members of the genera Streptomyces , Kitasatospora and Streptacidiphilus . The diagnostic cell-wall diamino acid of strain YIM DR4008T was found to be ll-diaminopimelic acid. The whole-cell hydrolysates contained a major amount of galactose and mannose along with a small proportion of fucose, glucose, rhamnose and ribose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylinositol mannosides and three unidentified phospholipids. The respiratory menaquinones were MK-9(H6) and MK-9(H8), while the major cellular fatty acids (>10 %) were anteiso-C15 : 0, C16 : 0, iso-C16 : 0, iso-C15 : 0 and anteiso-C17 : 0. The genomic DNA G+C content was determined to be 75.3 mol%. Based on the phenotypic, chemotaxonomic and molecular characteristics, strain YIM DR4008T is proposed to be recognized as a novel species of a new genus in the family Streptomycetaceae , with the name Allostreptomyces psammosilenae gen. nov., sp. nov. The type strain of the type species is YIM DR4008T (=DSM 42178T=CGMCC 4.7247T). An emended description of the family Streptomycetaceae is also provided.

A Gram-stain-positive, facultatively anaerobic actinomycete, designated strain T6220-5-2bT, was isolated from a sample taken from a mouldy spot on the surface of a mural painting (the white tiger, Byakko) inside the stone chamber of Takamatsuzuka Tumulus in Asuka village, Nara Prefecture, Japan. Based on 16S rRNA gene sequence analysis of the isolate, it was closely related to the genus Promicromonospora , but formed of a novel lineage within the family Promicromonosporaceae . The closest related species to strain T6220-5-2bT was Promicromonospora flava , with which it shared 99.1 % 16S rRNA gene sequence similarity. The isoprenoid quinone systems were menaquinones MK-9(H2), MK-9(H0) and MK-9(H4). The predominant cellular fatty acids for the isolate were anteiso-C15 : 0 and iso-C15 : 0. The peptidoglycan contained glutamic acid, aspartic acid, alanine and lysine, with the last named being the diagnostic diamino acid. The cell-wall acyl type was acetyl. The major polar lipids of the isolate were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside, two unknown phospholipids and an unknown phosphoglycolipid. Whole-cell sugars of the isolate were galactose, glucose and ribose. The DNA G+C content of the genomic DNA was 75.2 mol%. Based on the results of phylogenetic, physiological and biochemical analyses and DNA–DNA hybridization experiments, the isolate was considered to represent a novel species of a new genus in the family Promicromonosporaceae , for which the name Krasilnikoviella muralis gen. nov., sp. nov. is proposed. The type strain of Krasilnikoviella muralis is T6220-5-2bT (=JCM 28789T=NCIMB 15040T). The reclassification of Promicromonospora flava as Krasilnikoviella flava comb. nov. is also proposed with the emended description of this species.

A Gram-stain-positive, aerobic actinobacterial strain (designated SYP-A7299T), which displayed a rod–coccus growth lifecycle, was isolated from the rhizosphere of Ginkgo biloba L. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain SYP-A7299T belongs to the genus Arthrobacter and is most closely related to Arthrobacter halodurans JSM 078085T (97.4 % 16S rRNA gene sequence similarity). The DNA–DNA relatedness value between strain SYP-A7299T and A. halodurans JSM 078085T was 37 % ±2.9. The cell-wall peptidoglycan was A4α, and glucose and galactose were whole-cell sugars. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two glycolipids and an unknown polar lipid. The major menaquinone were MK-8(H2) (72 %) and MK-9(H2) (28 %), and the predominant cellular fatty acids were anteiso-C1 5  : 0, iso-C1 5  : 0 and anteiso-C1 7  : 0. The DNA G+C content was 68.9 mol%. Based on the morphological, physiological, biochemical and chemotaxonomic characters presented in this study, strain SYP-A7299T represents a novel species of the genus Arthrobacter , for which the name Arthrobacter ginkgonis sp. nov. is proposed. The type strain is SYP-A7299T (=DSM 100491T=KCTC 39 592T).

The aim of this study was to reclarify the taxonomic relationship among Streptomyces hygroscopicus subsp. hygroscopicus , Streptomyces endus and Streptomyces sporocinereus . Whole genome shotgun sequencing was performed for the type strains of these three taxa. Average nucleotide identity and digital DNA–DNA hybridization values among the three taxa were greater than the thresholds for bacterial species delineation, indicating that they belong to the same genomospecies. In addition, the phenotypic data previously reported also support the synonymy. Therefore, S. endus and S. sporocinereus should be reclassified as later heterotypic synonyms of S. hygroscopicus subsp. hygroscopicus .

A Gram-stain-positive, non-spore-forming actinobacterium, strain 9H-4T, isolated from the choana of a garden warbler (Sylvia borin) was studied to examine its taxonomic position. On the basis of 16S rRNA gene sequence analysis it was shown that strain 9H-4T belongs to the genus Aeromicrobium with Aeromicrobium flavum TYLN1T (98.7 % similarity) and Aeromicrobium tamlense SSW1-57T (98.4 %) as the nearest neighbours and forms a separate branch in a neighbour-joining phylogenetic tree based on 16S rRNA gene sequences. DNA–DNA hybridizations confirmed its novel species identity based on 39 and 46 % DNA–DNA relatedness with A. flavum DSM 19355T and A. tamlense DSM 19087T, respectively. The predominant menaquinone of strain 9H-4T was MK-9(H4). The peptidoglycan contained ll-diaminopimelic acid as the diagnostic diamino acid. The major fatty acids (C18 : 1 ω9c, 10-methyl C18 : 0, C16 : 0, C18 : 0, C16 : 0 2-OH) were consistent with the fatty acid patterns reported for members of the genus Aeromicrobium . The DNA G+C content of strain 9H-4T was 70.8 mol%. The distinct genotypic, chemotaxonomic, physiological and biochemical characteristics support the classification of strain 9H-4T as a representative of a novel species of the genus Aeromicrobium , for which the name Aeromicrobium choanae sp. nov. is proposed. The type strain is 9H-4T (=ZIM B1021T=LMG 29165T=CCM 8650T).

A Gram-staining-positive, strictly aerobic bacterium, designated R1T, was isolated from a rice paddy in South Korea. Cells were non-motile cocci showing oxidase-negative and catalase-positive activities. Growth of strain R1T was observed at 10–37 °C (optimum, 30 °C) and pH 5.0–9.0 (optimum, pH 7.0). Strain R1T contained iso-C16 : 0, summed feature 9 (comprising iso-C17 : 1ω9c/10-methyl C16  : 0), anteiso-C17 : 0, iso-C17 : 0 and anteiso-C17 : 1ω9c as the major fatty acids and MK-8 (H4) and MK-8 (H6) as the isoprenoid quinones. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, three unidentified phospholipids, four unidentified aminolipids, one unidentified glycolipid and four unidentified lipids. The peptidoglycan type was A4α with an l-Lys−l-Ser2−d-Glu interpeptide bridge containing a Gly residue. The G+C content of the genomic DNA was 64.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain R1T was most closely related to the members of the genus Flexivirga , Flexivirga endophytica YIM 7505T (97.7 %), Flexivirga luteaTBS-100T (97.5 %) and Flexivirga alba ST13T (97.2 %). On the basis of phenotypic, chemotaxonomic and molecular properties, it is clear that strain R1T represents a novel species of the genus Flexivirga , for which the name Flexivirga oryzae sp. nov. is proposed. The type strain is R1T (=KACC 18597T=JCM 31060T). An emended description of the genus Flexivirga is also proposed.

A novel Gram-staining-negative, aerobic, non-motile by gliding and rod-shaped strain, designated 22T, was isolated from surface-sterilized root tissue of maize planted in the Fangshan District of Beijing, PR China. The highest levels of 16S rRNA gene sequence similarity were found with respect to Pedobacter suwonensis 15-52T (97.5 %), Pedobacter terrae DS-57T (97.1 %) and Pedobacter alluvionis NWER-II11T (97.0 %). Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain 22T is a member of the genus Pedobacter . The isolate exhibited relatively low levels of DNA–DNA relatedness with respect to P. suwonensis DSM 18130T (21.3±2.0 %), P. alluvionis DSM 19624T (38.1±1.8 %) and P. terrae DSM 17933T (17.1±1.4 %). The DNA G+C content was 41.2±0.5 mol%. The major isoprenoid quinone was menaquinone-7 (MK-7). The major component in the polyamine pattern was sym-homospermidine. The major polar lipids consisted of phosphatidylethanolamine, three unidentified aminolipids and one unidentified lipid. The major fatty acids were identified as iso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The results of the physiological and biochemical tests and minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain 22T from the related species with high 16S rRNA gene sequence similarity, P. suwonensis DSM 18130T, P. alluvionis DSM 19624T and P. terrae DSM 17933T. Strain 22T represents a novel species within the genus Pedobacter , for which the name Pedobacter zeae sp. nov. is proposed, with the type strain 22T (=CGMCC 1.15287T=DSM 100774T).

In this paper, we report on the identification and species characterization of a novel Gram-staining-negative bacterium, strain S61T, isolated from the deep-sea sediment of the Okinawa Trough. Growth of strain S61T occurred at 4–37 °C (optimum, 25 °C), pH 6.0–10.0 (optimum, pH 7.0) and with 0–8 % (w/v) NaCl (optimum, 2 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S61T belonged to the genus Algoriphagus . The closest phylogenetic neighbours of strain S61T were Algoriphagus lutimaris S1-3T (98.1 %), Algoriphagus halophilus JC2051T (98.1 %), Algoriphagus chungangensis CAU 1002T (98.0 %) and Algoriphagus aestuarii MDM-1T (97.4 %). The sequence similarities between strain S61T and other close members of the genus were below 97 %. The values of DNA–DNA relatedness between strain S61T and its closest relatives in the genus Algoriphagus were well below 70 %. The genomic DNA G+C content of strain S61T was 40.2 mol%. The major menaquinone was MK-7. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids and one unidentified lipid. The major fatty acids were iso-C15 : 0 and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). Phylogenetic, physiological, biochemical, and morphological analyses suggested that this strain represents a novel species of the genus Algoriphagus , for which the name Algoriphagus iocasae sp. nov. is proposed with the type strain S61T (=KCTC 52359T=CCTCC AB 2015446T).

A bacterial strain, designated MIB-4T, was isolated from a freshwater spring in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain MIB-4T were Gram-staining-negative, aerobic, non-motile rods that formed pale pink colonies. Growth occurred at 15–30 °C (optimum, 25 °C), at pH 7–8 (optimum, pH 7) and with 0–0.5 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MIB-4T belonged to the genus Flectobacillus and was most closely related to Flectobacillus lacus CL-GP79T with sequence similarity of 98.7 %. Strain MIB-4T contained C16 : 1ω5c, iso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as the predominant fatty acids. The major isoprenoid quinone was MK-7. The polar lipid profile consisted of phosphatidylethanolamine, six unidentified aminophospholipids, one unidentified glycolipid, one unidentified phospholipid and three unidentified lipids. The DNA G+C content of the genomic DNA was 37.6 mol%. The DNA–DNA hybridization value for strain MIB-4T with F. lacus CL-GP79T was less than 32 %. On the basis of the phylogenetic inference and phenotypic data, strain MIB-4T should be classified as a representative of a novel species, for which the name Flectobacillus fontis sp. nov. is proposed. The type strain is MIB-4T (=BCRC 80926T=LMG 29292T=KCTC 33763T).

A novel Gram-stain-negative, non-spore-forming, orange-pigmented bacterium, designated strain SS2-56T, was isolated from sediment of the Nakdong River in Sangju-si, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate SS2-56T belongs to the family Chitinophagaceae , and was most closely related to Lacibacter daechungensis H32-4T (96.6 % similarity) and Lacibacter cauensis NJ-8T (96.1 %). Strain SS2-56T contained menaquinone 7 (MK-7) as a respiratory quinone and iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0 as major fatty acids. The major polar lipids of strain SS2-56T were phosphatidylethanolamine, one unidentified aminophospholipid and four unidentified lipids. The DNA G+C content was 43.8 mol%. Phenotypic, chemotaxonomic and phylogenetic evidence clearly indicates that strain SS2-56T represents a novel species of the genus Lacibacter , for which the name Lacibacter nakdongensis sp. nov. is proposed. The type strain is SS2-56T (=KCTC 52160T=JCM 31372T).

A Gram-stain-negative, non-spore-forming, rod-shaped and non-motile bacterium, designated strain xz20T, was isolated from the wastewater of a municipal wastewater treatment plant in Xuzhou, China. The taxonomic status of strain xz20T was determined using a polyphasic approach. Growth occurred at 15–40 °C (optimum, 25–37 °C), at pH 6.0–8.5 (optimum, pH 7.0) and with 0–3 % (w/v) NaCl (optimum, 1–2 %). 16S rRNA gene sequence analysis revealed that strain xz20T was a member of the genus Flavobacterium and shared the highest similarity with Flavobacterium . dongtanense LW30T (95.85 %) and Flavobacterium . haoranii LQY-7T (95.20 %). The major respiratory quinone of strain xz20T was menaquinone 6 (MK-6), and the major fatty acids were iso-C15 : 0, iso-C15 : 1 G and summed feature 9 (iso-C17 : 1 ω9c and/or C16 : 0 10-methyl). The major polar lipid was phosphatidylethanolamine. The genomic DNA G+C content of strain xz20T was 32.9 mol%. Based on the phenotypic, phylogenetic and chemotaxonomic characteristics, strain xz20T represents a novel species belonging to the genus Flavobacterium , for which the name Flavobacterium luticocti is proposed. The type strain is xz20T (=CCTCC AB 2015421T=JCM 31174T).

A novel Gram-stain-negative, aerobic, rod-shaped, non-flagellated and non-gliding bacterium, designated FA042T, was isolated from a marine sediment sample collected from the coast of Weihai, China (37° 32′ 02″ N 122° 03′ 44″ E). Optimal growth occurred at 33 °C, pH 7.0–7.5 and in the presence of 2–3 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain FA042T belonged to the genus Hyunsoonleella . The closest described neighbour, in terms of 16S rRNA gene sequence identity, was Hyunsoonleella jejuensis (95.0 %). The major fatty acids were iso-C15 : 0, iso-C15 : 1 G, C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 0 3-OH. The major respiratory quinone was MK-6. The major polar lipids of strain FA042T were phosphatidylethanolamine, three unidentified lipids and two unidentified aminolipids. The DNA G+C content was 38.5 mol%. Based on its phylogenetic and phenotypic characteristics, strain FA042T is presented as a representative of a novel species, for which the name Hyunsoonleella rubra sp. nov. is proposed. The type strain of Hyunsoonleella rubra sp. nov. is FA042T (=KCTC 42398T=MCCC 1H00110T).

The novel isolate belonging to the genus Terrimonas , designated CR94T, was isolated from rhizosphere soil of a ginseng field in Geumsan, Korea. Cells of strain CR94T were strictly aerobic, Gram-stain-negative, non-motile, non-filamentous single rods. Growth was observed at 10–37 °C (optimum 28 °C) and at pH 4.0–10.0 (optimum pH 6.0). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain CR94T belonged to the genus Terrimonas , showing highest sequence similarity to Terrimonas lutea DYT (97.3 %), Terrimonas pekingensis QHT (97.1 %), Terrimonas aquatica RIB1-6T (95.6 %), Terrimonas rubra M-8T (94.7 %) and Terrimonas ferruginea ATCC 13524T (93.8 %). DNA–DNA relatedness values between strain CR94T and T. lutea KACC 13047T, T. pekingensis KACC 18795T, T. ferruginea KACC 11310T and T. aquatica LMG 24825T were 30.5, 28.9, 17.8 and 13.5 %, respectively. The DNA G+C content was 46.5 mol% and the major respiratory quinone was menaquinone-7 (MK-7). The major cellular fatty acids of strain CR94T were iso-C15:1 G and iso-C15 : 0. On the basis of the polyphasic analysis, strain CR94T represents a novel species of the genus Terrimonas, for which the name Terrimonas rhizosphaerae sp. nov. is proposed. The type strain is CR94T (=KACC 17564T=NCAIM B 025317T).

Strain CBA3207T, a novel Gram-stain-negative, aerobic and rod-shaped bacterium, was isolated from the seashore sand of Jeju island in South Korea. Strain CBA3207T grew optimally at 25–30 °C and pH 7.0–7.5 with 3.0–4.0 % (w/v) NaCl. It was catalase-positive, oxidase-negative, and hydrolysed starch, gelatin, and Tweens 20, 40 and 80. The 16S rRNA gene sequence of strain CBA3207T showed 96.0, 95.6, 95.6, 95.5 and 95.5 % similarity to that of Aquimarinamytili PSC33T, Aquimarinaagarivorans HQM9T, Aquimarinalatercula DSM 2041T, Aquimarinaintermedia KMM 6258T and Aquimarinaamphilecti 92VT, respectively. The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G and summed feature 9 (10-methyl C16 : 0 and/or iso-C17 : 1 ω9c). The major respiratory quinone was menaquinone-6, and the major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and six unidentified lipids. The G+C content of the genomic DNA was 38.8 mol%. Based on phenotypic, genotypic and phylogenetic analyses, strain CBA3207T represents a novel species in the genus Aquimarina , for which the name Aquimarinaversatilissp. nov. is proposed. The type strain is CBA3207T (=KACC 17666T=JCM 19528T).

A Gram-stain-negative, facultatively anaerobic, orange-pigmented bacterium, designated HQYD1T, was isolated from a sea squirt (Styelaclava) and characterized using a polyphasic approach. Morphologically, strain HQYD1T exhibited rods with gliding motility. This novel isolate grew optimally at 28 °C in the presence of 2–3 % (w/v) NaCl. The 16S rRNA gene sequence was most similar to [ Saccharicrinis ] marinus Y11T (96.3 %), followed by Saccharicinis fermentans DSM 9555T (93.8 %). The dominant fatty acids of strain HQYD1T were identified as C16 : 0, C18 : 0 and iso-C15 : 0. Major polar lipids included an unidentified lipid and a phospholipid. The major respiratory quinone was found to be MK-7, and the genomic DNA G+C content was determined to be 35.1 mol%. Based on evidence from this taxonomic study, a novel genus, Labilibacter gen. nov., is proposed in the family Marinilabiliaceae with type species Labilibacter aurantiacus sp. nov. The type strain of the type species is HQYD1T (=MCCC 1K02304T=KCTC 42583T). As [ Saccharicrinis ] marinus Y11T clustered phylogenetically with strain HQYD1T, we also propose [ Saccharicrinis ] marinus Y11T be reclassified as Labilibacter marinus comb. nov. (type strain Y11T=CICC 10837T=KCTC 42400T).