1887

Abstract

A Gram-stain-positive, non-spore-forming actinobacterium, strain 9H-4, isolated from the choana of a garden warbler (Sylvia borin) was studied to examine its taxonomic position. On the basis of 16S rRNA gene sequence analysis it was shown that strain 9H-4 belongs to the genus Aeromicrobium with Aeromicrobium flavum TYLN1 (98.7 % similarity) and Aeromicrobium tamlense SSW1-57 (98.4 %) as the nearest neighbours and forms a separate branch in a neighbour-joining phylogenetic tree based on 16S rRNA gene sequences. DNA–DNA hybridizations confirmed its novel species identity based on 39 and 46 % DNA–DNA relatedness with A. flavum DSM 19355 and A. tamlense DSM 19087, respectively. The predominant menaquinone of strain 9H-4 was MK-9(H4). The peptidoglycan contained ll-diaminopimelic acid as the diagnostic diamino acid. The major fatty acids (C18 : 1 ω9c, 10-methyl C18 : 0, C16 : 0, C18 : 0, C16 : 0 2-OH) were consistent with the fatty acid patterns reported for members of the genus Aeromicrobium . The DNA G+C content of strain 9H-4 was 70.8 mol%. The distinct genotypic, chemotaxonomic, physiological and biochemical characteristics support the classification of strain 9H-4 as a representative of a novel species of the genus Aeromicrobium , for which the name Aeromicrobium choanae sp. nov. is proposed. The type strain is 9H-4 (=ZIM B1021=LMG 29165=CCM 8650).

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2019-10-21
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