1887

Abstract

A Gram-stain-positive actinobacterium, designated strain YIM DR4008, was isolated from the root sample of collected from Lijiang, Yunnan, China. Strain YIM DR4008 could grow at temperatures ranging from 10 to 50 °C (optimum 28–30 °C), at pH 5.0–11.0 (optimum pH 7.0) and in the presence of up to 4 % (w/v) NaCl. Sequence analysis of the 16S ribosomal RNA gene revealed that strain YIM DR4008 shared highest similarity (95.0 %) with NBRC 12779 and <95 % similarity with other known members of the genera , and The diagnostic cell-wall diamino acid of strain YIM DR4008 was found to be -diaminopimelic acid. The whole-cell hydrolysates contained a major amount of galactose and mannose along with a small proportion of fucose, glucose, rhamnose and ribose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylinositol mannosides and three unidentified phospholipids. The respiratory menaquinones were MK-9(H) and MK-9(H), while the major cellular fatty acids (>10 %) were anteiso-C, C, iso-C, iso-C and anteiso-C. The genomic DNA G+C content was determined to be 75.3 mol%. Based on the phenotypic, chemotaxonomic and molecular characteristics, strain YIM DR4008 is proposed to be recognized as a novel species of a new genus in the family , with the name gen. nov., sp. nov. The type strain of the type species is YIM DR4008 (=DSM 42178=CGMCC 4.7247). An emended description of the family is also provided.

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2017-02-01
2021-10-19
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