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Abstract

Strain JW12, isolated from surface seawater of the Arabian Sea, was subjected to characterization by a polyphasic taxonomic approach. Cells of the isolate were Gram-stain-negative, aerobic and rod-shaped. It accumulated poly-β-hydroxybutyrate. On the basis of 16S rRNA gene sequence analysis, strain JW12 was closely related to Alteromonas confluentis , with 16S rRNA gene sequence similarity of 98.0 %. Phylogenetic analysis revealed that it fell within the cluster of the genus Alteromonas and represented one independent lineage with A. confluentis . The average nucleotide identity (ANI) value and the genome-to-genome distance between strain JW12 and A. confluentis KCTC 42603 were 70.0 and 21.3 %, respectively. The sole respiratory quinone was ubiquinone-8 (Q8). The principal fatty acids were summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH), C16 : 0 and C18 : 1 ω7c. The major polar lipids included phosphatidylethanolamine, phosphatidylglycerol, two unidentified glycolipids and one aminophospholipid. The DNA G+C content was 48.4 mol%. Differential phylogenetic distinctiveness and chemotaxonomic differences, together with phenotypic properties obtained in this study, revealed that strain JW12 could be differentiated from the closely related species. Therefore, it is proposed that strain JW12 represents a novel species in the genus Alteromonas , for which the name Alteromonas lipolytica sp. nov. (type strain, JW12=CGMCC 1.15735=KCTC 52408=MCCC 1K03175), is proposed.

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2017-02-24
2019-10-23
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