1887

Abstract

Two Gram-stain-negative, aerobic, motile, halophilic, rod-shaped bacteria, designated Hb8 and Hb20, were isolated from a tidal flat environment located on the South-West Korean peninsula. The isolates grew at 10–37 °C, at pH 5.0–9.0 and in NaCl concentrations of 0.5–15 % (w/v; optimum, 3.0–6.0 %). Sequence analysis of the 16S rRNA indicated that the isolates belong to the genus Marinobacter and are most closely related to Marinobacter sediminum R65 (98.3 %), followed by Marinobacter lipolyticus SM19, Marinobacter salsuginis SD-14B and Marinobacter similis A3d10. The overall 16S rRNA gene sequence similarity with these species was 97.9 %, but Hb8 and Hb20 showed 100 % sequence similarity with each other. DNA–DNA relatedness values of H8 and Hb20 suggested that these isolates represent a single species, while DNA–DNA relatedness values of the two novel isolates with M. sediminum DSM 27079 and M. similis DSM 15400 were only 21.3 and 22.9 %, respectively. The major fatty acids present in strain Hb8 were identified as C16 : 0, C16 : 1ω9c, C18 : 1ω9c, C18 : 0 3-OH and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). Ubiquinone-9 was the main respiratory quinone in both the novel strains. The polar lipids found to be present included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, four unidentified phospholipids and five unidentified lipids. The genomic DNA G+C content of Hb8 and Hb20 was 54.5 mol%. Polyphasic analysis indicated that the two isolates are representatives of a novel species of the genus Marinobacte , for which the name Marinobacter salinus sp. nov. is proposed. The type strain is Hb8 (=KCTC 52255=JCM 31416).

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2017-02-24
2019-10-20
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