1887

Abstract

An alkalitolerant actinomycete strain, designated EGI 80674, was isolated from a rhizosphere soil of (Pall.) Bieb in Xinjiang, north-west China and subjected to a taxonomic characterization using a polyphasic approach. Strain EGI 80674 formed white aerial hyphae with long spore chains. Whole-cell hydrolysates of the isolate contained -diaminopimelic acid as the diagnostic diamino acid with no diagnostic sugars. The major fatty acids identified were iso-C, anteiso-C and 10-methyl-CTBSA. The predominant menaquinones detected were MK-10(H) and MK-10(H). The G+C content of the genomic DNA of strain EGI 80674 was 70.9 mol%. Strain EGI 80674 showed the highest 16S rRNA gene sequence similarity (97.24 %) to NBRC 102170. The DNA–DNA relatedness value of strain EGI 80674 and NBRC 102170 was 18.4±1.3 %. Phenotypical, chemotaxonomic and phylogenetic characteristics and DNA–DNA hybridization data suggest that strain EGI 80674 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EGI 80674 (=CGMCC 4.7228=KCTC 39673).

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2016-12-01
2019-12-12
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References

  1. Akhwale J. K., Göker M., Rohde M., Schumann P., Boga H. I., Klenk H. P.. 2016; Nocardiopsis mwathae sp. nov., isolated from the haloalkaline Lake Elmenteita in the African Rift Valley. Antonie Van Leeuwenhoek109:421–430 [CrossRef][PubMed]
    [Google Scholar]
  2. Bennur T., Kumar A. R., Zinjarde S., Javdekar V.. 2015; Nocardiopsis species: Incidence, ecological roles and adaptations. Microbiol Res174:33–47 [CrossRef][PubMed]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  4. Ding Z.-G., Li M.-G., Zhao J.-Y., Ren J., Huang R., Xie M.-J., Cui X.-L., Zhu H.-J., Wen M.-L.. 2010; Naphthospironone a: an unprecedented and highly functionalized polycyclic metabolite from an alkaline mine waste extremophile. Chemistry16:3902–3905 [CrossRef][PubMed]
    [Google Scholar]
  5. Ding Z.-G., Zhao J.-Y., Li M.-G., Huang R., Li Q. M., Cui X.-L., Zhu H.-J., Wen M.-L.. 2012; Griseusins F and G, spiro-naphthoquinones from a tin mine tailings-derived alkalophilic Nocardiopsis species. J Nat Prod75:1994–1998 [CrossRef][PubMed]
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E.. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bactriol39:224–229 [CrossRef]
    [Google Scholar]
  7. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  8. Felsenstein J.. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution39:783–789 [CrossRef]
    [Google Scholar]
  9. Fitch W. M.. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool20:406–416[CrossRef]
    [Google Scholar]
  10. Goodfellow M.. 1971; Numerical taxonomy of some nocardioform bacteria. J Gen Microbiol69:33–80 [CrossRef][PubMed]
    [Google Scholar]
  11. Horikoshi K.. 1999; Alkaliphiles: some applications of their products for biotechnology. Microbiol Mol Biol Rev63:735–750[PubMed]
    [Google Scholar]
  12. Hozzein W. N., Trujillo M. E.. 2012; Genus I. Nocardiopsis Meyer 1976, 487AL. In Bergey’s Manual of Systematic Bacteriology, 2nd edn.vol. 5 pp.1891–1906Edited by Whitman W. B., Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Ludwig W., Suzuki K., Parte A.. New York: Springer;
    [Google Scholar]
  13. Kelly K. L.. 1964; Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office;
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  15. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L.. 2007; Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol57:1424–1428 [CrossRef][PubMed]
    [Google Scholar]
  16. Marmur J.. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol3:208–218 [CrossRef]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B.. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bactriol39:159–167 [CrossRef]
    [Google Scholar]
  18. Meyer J.. 1976; Nocardiopsis, a new genus of the order Actinomycetales. Int J Syst Bactriol26:487–493 [CrossRef]
    [Google Scholar]
  19. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2:233–241 [CrossRef]
    [Google Scholar]
  20. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E.. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol46:1088–1092 [CrossRef][PubMed]
    [Google Scholar]
  21. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  22. Sasser M.. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101. Newark, DE: MIDI Inc;
    [Google Scholar]
  23. Shirling E. B., Gottlieb D.. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol16:313–340 [CrossRef]
    [Google Scholar]
  24. Stackebrandt E., Goebel B. M.. 1994; Taxonomic note: a place for DNADNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bactriol44:846–849 [CrossRef]
    [Google Scholar]
  25. Staneck J. L., Roberts G. D.. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol28:226–231[PubMed]
    [Google Scholar]
  26. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  27. Tang S. K., Tian X. P., Zhi X. Y., Cai M., Wu J. Y., Yang L. L., Xu L. H., Li W. J.. 2008; Haloactinospora alba gen. nov., sp. nov., a halophilic filamentous actinomycete of the family Nocardiopsaceae. Int J Syst Evol Microbiol58:2075–2080 [CrossRef][PubMed]
    [Google Scholar]
  28. Tang S. K., Wang Y., Chen Y., Lou K., Cao L. L., Xu L. H., Li W. J.. 2009; Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella. Int J Syst Evol Microbiol59:2025–2033 [CrossRef][PubMed]
    [Google Scholar]
  29. Williams S. T., Goodfellow M., Alderson G., Wellington E. M., Sneath P. H., Sackin M. J.. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol129:1743–1813 [CrossRef][PubMed]
    [Google Scholar]
  30. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G.-Z., Chen H. H., Xu L. H., Jiang C. L.. 2005; Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol55:1149–1153 [CrossRef][PubMed]
    [Google Scholar]
  31. Yamamura H., Ohkubo S. Y., Ishida Y., Otoguro M., Tamura T., Hayakawa M.. 2010; Nocardiopsis nikkonensis sp. nov., isolated from a compost sample. Int J Syst Evol Microbiol60:2967–2971 [CrossRef][PubMed]
    [Google Scholar]
  32. Zhang D. F., Pan H. Q., He J., Zhang X. M., Zhang Y. G., Klenk H. P., Hu J. C., Li W. J.. 2013; Description of Streptomonospora sediminis sp. nov. and Streptomonospora nanhaiensis sp. nov., and reclassification of Nocardiopsis arabia Hozzein & Goodfellow 2008 as Streptomonospora arabica comb. nov. and emended description of the genus Streptomonospora. Int J Syst Evol Microbiol63:4447–4455 [CrossRef][PubMed]
    [Google Scholar]
  33. Zhang Y. G., Liu Q., Wang H. F., Park D. J., Guo J. W., Kim C. J., Zhang Y. M., Li W. J.. 2016; Nocardiopsis ansamitocini sp. nov., a new producer of ansamitocin P-3 of the genus Nocardiopsis. Int J Syst Evol Microbiol66:230–235 [CrossRef][PubMed]
    [Google Scholar]
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