1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 66, Issue 12

sp. nov., isolated from rhizosphere soil of (Pall.) Bieb Free

Abstract

An alkalitolerant actinomycete strain, designated EGI 80674, was isolated from a rhizosphere soil of (Pall.) Bieb in Xinjiang, north-west China and subjected to a taxonomic characterization using a polyphasic approach. Strain EGI 80674 formed white aerial hyphae with long spore chains. Whole-cell hydrolysates of the isolate contained -diaminopimelic acid as the diagnostic diamino acid with no diagnostic sugars. The major fatty acids identified were iso-C, anteiso-C and 10-methyl-CTBSA. The predominant menaquinones detected were MK-10(H) and MK-10(H). The G+C content of the genomic DNA of strain EGI 80674 was 70.9 mol%. Strain EGI 80674 showed the highest 16S rRNA gene sequence similarity (97.24 %) to NBRC 102170. The DNA–DNA relatedness value of strain EGI 80674 and NBRC 102170 was 18.4±1.3 %. Phenotypical, chemotaxonomic and phylogenetic characteristics and DNA–DNA hybridization data suggest that strain EGI 80674 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EGI 80674 (=CGMCC 4.7228=KCTC 39673).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Halocnermum strobilaceum (Pall.) Bieb , Nocardiopsis rhizosphaerae sp. nov. and rhizosphere soil
© 2016 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001483
2016-12-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/5129.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001483&mimeType=html&fmt=ahah

References

  1. Akhwale J. K., Göker M., Rohde M., Schumann P., Boga H. I., Klenk H. P. 2016; Nocardiopsis mwathae sp. nov., isolated from the haloalkaline Lake Elmenteita in the African Rift Valley. Antonie Van Leeuwenhoek 109:421–430 [View Article][PubMed]
     [Google Scholar]
  2. Bennur T., Kumar A. R., Zinjarde S., Javdekar V. 2015; Nocardiopsis species: Incidence, ecological roles and adaptations. Microbiol Res 174:33–47 [View Article][PubMed]
     [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [View Article][PubMed]
     [Google Scholar]
  4. Ding Z.-G., Li M.-G., Zhao J.-Y., Ren J., Huang R., Xie M.-J., Cui X.-L., Zhu H.-J., Wen M.-L. 2010; Naphthospironone a: an unprecedented and highly functionalized polycyclic metabolite from an alkaline mine waste extremophile. Chemistry 16:3902–3905 [View Article][PubMed]
     [Google Scholar]
  5. Ding Z.-G., Zhao J.-Y., Li M.-G., Huang R., Li Q. M., Cui X.-L., Zhu H.-J., Wen M.-L. 2012; Griseusins F and G, spiro-naphthoquinones from a tin mine tailings-derived alkalophilic Nocardiopsis species. J Nat Prod 75:1994–1998 [View Article][PubMed]
     [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bactriol 39:224–229 [View Article]
     [Google Scholar]
  7. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
     [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789 [View Article]
     [Google Scholar]
  9. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
     [Google Scholar]
  10. Goodfellow M. 1971; Numerical taxonomy of some nocardioform bacteria. J Gen Microbiol 69:33–80 [View Article][PubMed]
     [Google Scholar]
  11. Horikoshi K. 1999; Alkaliphiles: some applications of their products for biotechnology. Microbiol Mol Biol Rev 63:735–750[PubMed]
     [Google Scholar]
  12. Hozzein W. N., Trujillo M. E. 2012; Genus I. Nocardiopsis Meyer 1976, 487AL. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 5 pp. 1891–1906Edited by Whitman W. B., Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Ludwig W., Suzuki K., Parte A. New York: Springer;
     [Google Scholar]
  13. Kelly K. L. 1964 Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office;
     [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
     [Google Scholar]
  15. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L. 2007; Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia . Int J Syst Evol Microbiol 57:1424–1428 [View Article][PubMed]
     [Google Scholar]
  16. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [View Article]
     [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bactriol 39:159–167 [View Article]
     [Google Scholar]
  18. Meyer J. 1976; Nocardiopsis, a new genus of the order Actinomycetales . Int J Syst Bactriol 26:487–493 [View Article]
     [Google Scholar]
  19. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  20. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [View Article][PubMed]
     [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  22. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101. Newark, DE: MIDI Inc;
     [Google Scholar]
  23. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
     [Google Scholar]
  24. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bactriol 44:846–849 [View Article]
     [Google Scholar]
  25. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231[PubMed]
     [Google Scholar]
  26. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  27. Tang S. K., Tian X. P., Zhi X. Y., Cai M., Wu J. Y., Yang L. L., Xu L. H., Li W. J. 2008; Haloactinospora alba gen. nov., sp. nov., a halophilic filamentous actinomycete of the family Nocardiopsaceae . Int J Syst Evol Microbiol 58:2075–2080 [View Article][PubMed]
     [Google Scholar]
  28. Tang S. K., Wang Y., Chen Y., Lou K., Cao L. L., Xu L. H., Li W. J. 2009; Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella . Int J Syst Evol Microbiol 59:2025–2033 [View Article][PubMed]
     [Google Scholar]
  29. Williams S. T., Goodfellow M., Alderson G., Wellington E. M., Sneath P. H., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813 [View Article][PubMed]
     [Google Scholar]
  30. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G.-Z., Chen H. H., Xu L. H., Jiang C. L. 2005; Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153 [View Article][PubMed]
     [Google Scholar]
  31. Yamamura H., Ohkubo S. Y., Ishida Y., Otoguro M., Tamura T., Hayakawa M. 2010; Nocardiopsis nikkonensis sp. nov., isolated from a compost sample. Int J Syst Evol Microbiol 60:2967–2971 [View Article][PubMed]
     [Google Scholar]
  32. Zhang D. F., Pan H. Q., He J., Zhang X. M., Zhang Y. G., Klenk H. P., Hu J. C., Li W. J. 2013; Description of Streptomonospora sediminis sp. nov. and Streptomonospora nanhaiensis sp. nov., and reclassification of Nocardiopsis arabia Hozzein & Goodfellow 2008 as Streptomonospora arabica comb. nov. and emended description of the genus Streptomonospora . Int J Syst Evol Microbiol 63:4447–4455 [View Article][PubMed]
     [Google Scholar]
  33. Zhang Y. G., Liu Q., Wang H. F., Park D. J., Guo J. W., Kim C. J., Zhang Y. M., Li W. J. 2016; Nocardiopsis ansamitocini sp. nov., a new producer of ansamitocin P-3 of the genus Nocardiopsis . Int J Syst Evol Microbiol 66:230–235 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001483
Loading
Nocardiopsis rhizosphaerae sp. nov., isolated from rhizosphere soil of Halocnermum strobilaceum (Pall.) Bieb
Int J Syst Evol Microbiol 66, 5129 (2016); https://doi.org/10.1099/ijsem.0.001483
/content/journal/ijsem/10.1099/ijsem.0.001483
/content/journal/ijsem/10.1099/ijsem.0.001483
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error