RT Journal Article SR Electronic(1) A1 Zhang, Yong-Guang A1 Lu, Xin-Hua A1 Ding, Yan-Bo A1 Zhou, Xing-Kui A1 Wang, Hong-Fei A1 Guo, Jian-Wei A1 Liu, Yong-Hong A1 Duan, Yan-Qing A1 Li, Wen-JunYR 2016 T1 Nocardiopsis rhizosphaerae sp. nov., isolated from rhizosphere soil of Halocnermum strobilaceum (Pall.) Bieb JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 12 SP 5129 OP 5133 DO https://doi.org/10.1099/ijsem.0.001483 PB Microbiology Society, SN 1466-5034, AB An alkalitolerant actinomycete strain, designated EGI 80674T, was isolated from a rhizosphere soil of Halocnermumstrobilaceum (Pall.) Bieb in Xinjiang, north-west China and subjected to a taxonomic characterization using a polyphasic approach. Strain EGI 80674T formed white aerial hyphae with long spore chains. Whole-cell hydrolysates of the isolate contained meso-diaminopimelic acid as the diagnostic diamino acid with no diagnostic sugars. The major fatty acids identified were iso-C16 : 0, anteiso-C17 : 0 and 10-methyl-C18 : 0TBSA. The predominant menaquinones detected were MK-10(H8) and MK-10(H6). The G+C content of the genomic DNA of strain EGI 80674T was 70.9 mol%. Strain EGI 80674T showed the highest 16S rRNA gene sequence similarity (97.24 %) to Nocardiopsis nikkonensis NBRC 102170T. The DNA–DNA relatedness value of strain EGI 80674T and N. nikkonensis NBRC 102170T was 18.4±1.3 %. Phenotypical, chemotaxonomic and phylogenetic characteristics and DNA–DNA hybridization data suggest that strain EGI 80674T represents a novel species of the genus Nocardiopsis , for which the name Nocardiopsis rhizosphaerae sp. nov. is proposed. The type strain is EGI 80674T (=CGMCC 4.7228T=KCTC 39673T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001483