1887

Abstract

A Gram-stain-negative, aerobic, rod-shaped, motile bacterium, strain B1555-1, was isolated from an ice core drilled from Ulugh Muztagh Glacier, China. The optimum growth temperature of strain B1555-1 was 15 °C and optimum pH was 7. The major fatty acids of strain B1555-1 were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The predominant respiratory quinone was Q-8. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain B1555-1 was 66.0 mol%. In 16S rRNA gene sequence comparisons, strain B1555-1 was affiliated to the genus Massilia and shared 98.30 and 97.13 % similarity with Massilia eurypsychrophila B528-3 and Massilia niabensis 5420S-26, respectively. The results of DNA–DNA hybridization revealed that strain B1555-1 showed 49.8 % relatedness with M. eurypsychrophila B528-3 and 38.5 % with M. niabensis 5420S-26. Based on the genotypic and phenotypic evidence presented in this study, strain B1555-1 represents a novel species of the genus Massilia , for which the name Massilia psychrophila sp. nov. is proposed. The type strain is B1555-1 (=CGMCC 1.15196=JCM 30813).

Keyword(s): ice core , Massilia and novel species
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001315
2016-10-01
2019-09-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/10/4088.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001315&mimeType=html&fmt=ahah

References

  1. Breznak J. A., Costilow R. N..( 1994;). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology , pp. 137–154. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  2. Du Y., Yu X., Wang G..( 2012;). Massilia tieshanensis sp. nov., isolated from mining soil. . Int J Syst Evol Microbiol 62: 2356–2362. [CrossRef] [PubMed]
    [Google Scholar]
  3. Embley T. M..( 1991;). The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. . Lett Appl Microbiol 13: 171–174. [CrossRef] [PubMed]
    [Google Scholar]
  4. Feng G. D., Yang S. Z., Li H. P., Zhu H. H..( 2016;). Massilia putida sp. nov., a dimethyl disulfide-producing bacterium isolated from wolfram mine tailing. . Int J Syst Evol Microbiol 66: 50–55. [CrossRef] [PubMed]
    [Google Scholar]
  5. Gallego V., Sánchez-Porro C., García M. T., Ventosa A., Garcıa M. T..( 2006;). Massilia aurea sp. nov., isolated from drinking water. . Int J Syst Evol Microbiol 56: 2449–2453. [CrossRef] [PubMed]
    [Google Scholar]
  6. Hiraishi A., Ueda Y., Ishihara J..( 1998;). Quinone profiling of bacterial communities in natural and synthetic sewage activated sludge for enhanced phosphate removal. . App Environ Microbiol 64: 992–998.
    [Google Scholar]
  7. Kämpfer P., Falsen E., Busse H. J..( 2008;). Naxibacter varians sp. nov. and Naxibacter haematophilus sp. nov., and emended description of the genus Naxibacter. . Int J Syst Evol Microbiol 58: 1680–1684. [CrossRef] [PubMed]
    [Google Scholar]
  8. Kämpfer P., Lodders N., Martin K., Falsen E..( 2011;). Revision of the genus Massilia La Scola et al. 2000, with an emended description of the genus and inclusion of all species of the genus Naxibacter as new combinations, and proposal of Massilia consociata sp. nov. . Int J Syst Evol Microbiol 61: 1528–1533. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kämpfer P., Lodders N., Martin K., Falsen E..( 2012;). Massilia oculi sp. nov., isolated from a human clinical specimen. . Int J Syst Evol Microbiol 62: 364–369. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kates M..( 1986;). Techniques of Lipidology Isolation, Analysis and Identification of Lipids, , 2nd edn. rev. , pp. 106241–107246. Amsterdam:: Elsevier;.
    [Google Scholar]
  11. Kim J..( 2014;). Massilia kyonggiensis sp. nov., isolated from forest soil in Korea. . J Microbiol 52: 378–383. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al.( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kong B. H., Li Y. H., Liu M., Liu Y., Li C. L., Liu L., Yang Z. W., Yu R..( 2013;). Massilia namucuonensis sp. nov., isolated from a soil sample. . Int J Syst Evol Microbiol 63: 352–357. [CrossRef] [PubMed]
    [Google Scholar]
  14. La Scola B., Birtles R. J., Mallet M. N., Raoult D..( 1998;). Massilia timonae gen. nov., sp. nov., isolated from blood of an immunocompromised patient with cerebellar lesions. . J Clin Microbiol 36: 2847–2852.[PubMed]
    [Google Scholar]
  15. Luo X., Xie Q., Wang J., Pang H., Fan J., Zhang J..( 2013;). Massilia lurida sp. nov., isolated from soil. . Int J Syst Evol Microbiol 63: 2118–2123. [CrossRef] [PubMed]
    [Google Scholar]
  16. Marmur J..( 1961;). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3: 208–218. [CrossRef]
    [Google Scholar]
  17. Marmur J., Doty P..( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5: 109–118. [CrossRef] [PubMed]
    [Google Scholar]
  18. Orthová I., Kämpfer P., Glaeser S. P., Kaden R., Busse H. J..( 2015;). Massilia norwichensis sp. nov., isolated from an air sample. . Int J Syst Evol Microbiol 65: 56–64. [CrossRef] [PubMed]
    [Google Scholar]
  19. Reasoner D. J., Geldreich E. E..( 1985;). A new medium for the enumeration and subculture of bacteria from potable water. . App Environ Microbiol 49: 1–7.
    [Google Scholar]
  20. Rodríguez-Díaz M., Cerrone F., Sánchez-Peinado M., SantaCruz-Calvo L., Pozo C., López J. G..( 2014;). Massilia umbonata sp. nov., able to accumulate poly-β-hydroxybutyrate, isolated from a sewage sludge compost-soil microcosm. . Int J Syst Evol Microbiol 64: 131–137. [CrossRef] [PubMed]
    [Google Scholar]
  21. Romano I., Lama L., Nicolaus B., Poli A., Gambacorta A., Giordano A..( 2006;). Halomonas alkaliphila sp. nov., a novel halotolerant alkaliphilic bacterium isolated from a salt pool in Campania (Italy). . J Gen Appl Microbiol 52: 339–348. [CrossRef] [PubMed]
    [Google Scholar]
  22. Shen L., Liu Y., Wang N., Yao T., Jiao N., Liu H., Zhou Y., Xu B., Liu X..( 2013;). Massilia yuzhufengensis sp. nov., isolated from an ice core. . Int J Syst Evol Microbiol 63: 1285–1290. [CrossRef] [PubMed]
    [Google Scholar]
  23. Shen L., Liu Y., Gu Z., Xu B., Wang N., Jiao N., Liu H., Zhou Y..( 2015;). Massilia eurypsychrophila sp. nov. a facultatively psychrophilic bacteria isolated from ice core. . Int J Syst Evol Microbiol 65: 2124–2129. [CrossRef] [PubMed]
    [Google Scholar]
  24. Singh H., Du J., Won K., Yang J. E., Yin C., Kook M., Yi T. H..( 2015;). Massilia arvi sp. nov., isolated from fallow-land soil previously cultivated with Brassica oleracea, and emended description of the genus Massilia. . Int J Syst Evol Microbiol 65: 3690–3696. [CrossRef] [PubMed]
    [Google Scholar]
  25. Smibert R. M., Krieg N. R..( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  26. Spiess M., Huintjes E., Schneider C..( 2016;). Comparison of modelled- and remote sensing- derived daily snow line altitudes at Ulugh Muztagh, northern Tibetan Plateau. . J Mt Sci 13: 593–613. [CrossRef]
    [Google Scholar]
  27. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S..( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30: 2725–2729. [CrossRef] [PubMed]
    [Google Scholar]
  28. Wang J., Zhang J., Pang H., Zhang Y., Li Y., Fan J..( 2012;). Massilia flava sp. nov., isolated from soil. . Int J Syst Evol Microbiol 62: 580–585. [CrossRef] [PubMed]
    [Google Scholar]
  29. Weon H. Y., Kim B. Y., Son J. A., Jang H. B., Hong S. K., Go S. J., Kwon S. W..( 2008;). Massilia aerilata sp. nov., isolated from an air sample. . Int J Syst Evol Microbiol 58: 1422–1425. [CrossRef] [PubMed]
    [Google Scholar]
  30. Weon H. Y., Kim B. Y., Hong S. B., Jeon Y. A., Koo B. S., Kwon S. W., Stackebrandt E..( 2009;). Massilia niabensis sp. nov. and Massilia niastensis sp. nov., isolated from air samples. . Int J Syst Evol Microbiol 59: 1656–1660. [CrossRef] [PubMed]
    [Google Scholar]
  31. Weon H.-Y., Yoo S.-H., Kim S.-J., Kim Y.-S., Anandham R., Kwon S.-W..( 2010;). Massilia jejuensis sp. nov. and Naxibacter suwonensis sp. nov., isolated from air samples. . Int J Syst Evol Microbiol 60: 1938–1943. [CrossRef]
    [Google Scholar]
  32. Zhang Y. Q., Li W. J., Zhang K. Y., Tian X. P., Jiang Y., Xu L. H., Jiang C. L., Lai R..( 2006;). Massilia dura sp. nov., Massilia albidiflava sp. nov., Massilia plicata sp. nov. and Massilia lutea sp. nov., isolated from soils in China. . Int J Syst Evol Microbiol 56: 459–463. [CrossRef] [PubMed]
    [Google Scholar]
  33. Zul D., Wanner G., Overmann J..( 2008;). Massilia brevitalea sp. nov., a novel betaproteobacterium isolated from lysimeter soil. . Int J Syst Evol Microbiol 58: 1245–1251. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001315
Loading
/content/journal/ijsem/10.1099/ijsem.0.001315
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error