@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001315, author = "Guo, Bixi and Liu, Yongqin and Gu, Zhengquan and Shen, Liang and Liu, Keshao and Wang, Ningliang and Xing, Tingting and Liu, Hongcan and Zhou, Yuguang and Li, Jiule", title = "Massilia psychrophila sp. nov., isolated from an ice core", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "10", pages = "4088-4093", doi = "https://doi.org/10.1099/ijsem.0.001315", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001315", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Massilia", keywords = "ice core", keywords = "novel species", abstract = "A Gram-stain-negative, aerobic, rod-shaped, motile bacterium, strain B1555-1T, was isolated from an ice core drilled from Ulugh Muztagh Glacier, China. The optimum growth temperature of strain B1555-1T was 15 °C and optimum pH was 7. The major fatty acids of strain B1555-1T were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The predominant respiratory quinone was Q-8. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain B1555-1T was 66.0 mol%. In 16S rRNA gene sequence comparisons, strain B1555-1T was affiliated to the genus Massilia and shared 98.30 and 97.13 % similarity with Massilia eurypsychrophila B528-3T and Massilia niabensis 5420S-26T, respectively. The results of DNA–DNA hybridization revealed that strain B1555-1T showed 49.8 % relatedness with M. eurypsychrophila B528-3T and 38.5 % with M. niabensis 5420S-26T. Based on the genotypic and phenotypic evidence presented in this study, strain B1555-1T represents a novel species of the genus Massilia , for which the name Massilia psychrophila sp. nov. is proposed. The type strain is B1555-1T (=CGMCC 1.15196T=JCM 30813T).", }