1887

Abstract

A novel Gram-stain-negative, non-motile, non-spore-forming, non-flagellated, aerobic, beige-coloured and rod-shaped bacterium, designated strain KHS02, was isolated from the intestinal tract of a Japanese flying squid, , which was collected from the East Sea, Korea. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain KHS02 formed a monophyletic clade with HDW-32, with which it had the highest sequence similarity (98.67 %). Strain KHS02 grew optimally at pH 7 with 2 % (w/v) NaCl at 25 °C on marine broth 2216, but could not grow without Na. The predominant isoprenoid quinone was ubiquinone-10 (Q-10). The major fatty acids (>10 % of the total) were summed feature 8 (Cω6 and/or Cω7) and 11-methyl Cω7. The polar lipids of strain KHS02 comprised phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminolipids, an unidentified phospholipid and an unidentified lipid. The genomic DNA G+C content was 58.6 mol%. DNA–DNA hybridization showed that the isolate shared 16.2 ± 1.3 % (reciprocal, 15.7 ± 2.8 %) genomic relatedness with the type strain of the closest species. In conclusion, this isolate is suggested to represent a novel species of the genus , for which the name is proposed. The type strain is KHS02 ( = KACC 18257 = JCM 30419).

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2016-03-01
2019-10-21
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References

  1. Bae J. W. , Park Y. H. . ( 2006;). Homogeneous versus heterogeneous probes for microbial ecological microarrays. Trends Biotechnol 24: 318–323 [CrossRef] [PubMed].
    [Google Scholar]
  2. Bae J. W. , Rhee S. K. , Nam Y. D. , Park Y. H. . ( 2005;). Generation of subspecies level-specific microbial diagnostic microarrays using genes amplified from subtractive suppression hybridization as microarray probes. Nucleic Acids Res 33: e113 [CrossRef] [PubMed].
    [Google Scholar]
  3. Belas R. , Mileham A. , Cohn D. , Hilman M. , Simon M. , Silverman M. . ( 1982;). Bacterial bioluminescence: isolation and expression of the luciferase genes from Vibrio harveyi . Science 218: 791–793 [CrossRef] [PubMed].
    [Google Scholar]
  4. Chang H. W. , Nam Y. D. , Jung M. Y. , Kim K. H. , Roh S. W. , Kim M. S. , Jeon C. O. , Yoon J. H. , Bae J. W. . ( 2008a;). Statistical superiority of genome-probing microarrays as genomic DNA-DNA hybridization in revealing the bacterial phylogenetic relationship compared to conventional methods. J Microbiol Methods 75: 523–530 [CrossRef] [PubMed].
    [Google Scholar]
  5. Chang H. W. , Sung Y. , Kim K. H. , Nam Y. D. , Roh S. W. , Kim M. S. , Jeon C. O. , Bae J. W. . ( 2008b;). Development of microbial genome-probing microarrays using digital multiple displacement amplification of uncultivated microbial single cells. Environ Sci Technol 42: 6058–6064 [CrossRef] [PubMed].
    [Google Scholar]
  6. Collins M. D. , Jones D. . ( 1981a;). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 45: 316–354,[PubMed].
    [Google Scholar]
  7. Collins M. D. , Jones D. . ( 1981b;). A note on the separation of natural mixtures of bacterial ubiquinones using reverse-phase partition thin-layer chromatography and high performance liquid chromatography. J Appl Bacteriol 51: 129–134 [CrossRef] [PubMed].
    [Google Scholar]
  8. Felsenstein J. . ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  9. Gonzalez J. M. , Saiz-Jimenez C. . ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4: 770–773 [CrossRef] [PubMed].
    [Google Scholar]
  10. Hyun D. W. , Kim J. Y. , Kim M. S. , Shin N. R. , Kim H. S. , Lee J. Y. , Bae J. W. . ( 2015;). Actibacter haliotis sp. nov., isolated from the gut of an abalone, Haliotis discus hannai, and emended description of the genus Actibacter . Int J Syst Evol Microbiol 65: 49–55 [CrossRef] [PubMed].
    [Google Scholar]
  11. Jung Y. T. , Park S. , Lee J. S. , Oh T. K. , Yoon J. H. . ( 2012;). Pseudahrensia aquimaris gen. nov., sp. nov., isolated from seawater. Int J Syst Evol Microbiol 62: 2056–2061 [CrossRef] [PubMed].
    [Google Scholar]
  12. Kaplan H. B. , Greenberg E. P. . ( 1985;). Diffusion of autoinducer is involved in regulation of the Vibrio fischeri luminescence system. J Bacteriol 163: 1210–1214,[PubMed].
    [Google Scholar]
  13. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  14. Kluge A. G. , Farris F. S. . ( 1969;). Quantitative phyletics and the evolution of anurans. Syst Zool 18: 1–32 [CrossRef].
    [Google Scholar]
  15. Lane R. J. . ( 1991;). Vascular surgery for vasculogenic impotence. Med J Aust 155: 547–548,[PubMed].
    [Google Scholar]
  16. MIDI ( 1999;). Sherlock Microbial Identification System Operating Manual, version 3.0., Newark, DE: MIDI, Inc;.
    [Google Scholar]
  17. Peix A. , Rivas R. , Trujillo M. E. , Vancanneyt M. , Velázquez E. , Willems A. . ( 2005;). Reclassification of Agrobacterium ferrugineum LMG 128 as Hoeflea marina gen. nov., sp. nov. Int J Syst Evol Microbiol 55: 1163–1166 [CrossRef] [PubMed].
    [Google Scholar]
  18. Rochelle P. A. , Fry J. C. , Parkes R. J. , Weightman A. J. . ( 1992;). DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities. FEMS Microbiol Lett 100: 59–65 [CrossRef] [PubMed].
    [Google Scholar]
  19. Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425,[PubMed].
    [Google Scholar]
  20. Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  21. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  22. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–4882 [CrossRef] [PubMed].
    [Google Scholar]
  23. Tindall B. J. . ( 1990;). Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66: 199–202 [CrossRef].
    [Google Scholar]
  24. Tittsler R. P. , Sandholzer L. A. . ( 1936;). The use of semi-solid agar for the detection of bacterial motility. J Bacteriol 31: 575–580 [PubMed].
    [Google Scholar]
  25. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463–464 [CrossRef].
    [Google Scholar]
  26. Xin H. , Itoh T. , Zhou P. , Suzuki K. , Kamekura M. , Nakase T. . ( 2000;). Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. Int J Syst Evol Microbiol 50: 1297–1303.[CrossRef]
    [Google Scholar]
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