RT Journal Article SR Electronic(1) A1 Kim, Hyun Sik A1 Kim, Pil Soo A1 Hyun, Dong-Wook A1 Lee, June-Young A1 Kang, Woorim A1 Shin, Na-Ri A1 Whon, Tae Woong A1 Bae, Jin-WooYR 2016 T1 Pseudahrensia todarodis sp. nov., isolated from the gut of a Japanese flying squid, Todarodes pacificus JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 3 SP 1389 OP 1393 DO https://doi.org/10.1099/ijsem.0.000888 PB Microbiology Society, SN 1466-5034, AB A novel Gram-stain-negative, non-motile, non-spore-forming, non-flagellated, aerobic, beige-coloured and rod-shaped bacterium, designated strain KHS02T, was isolated from the intestinal tract of a Japanese flying squid, Todarodes pacificus, which was collected from the East Sea, Korea. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain KHS02T formed a monophyletic clade with Pseudahrensia aquimaris HDW-32T, with which it had the highest sequence similarity (98.67 %). Strain KHS02T grew optimally at pH 7 with 2 % (w/v) NaCl at 25 °C on marine broth 2216, but could not grow without Na+. The predominant isoprenoid quinone was ubiquinone-10 (Q-10). The major fatty acids (>10 % of the total) were summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c) and 11-methyl C18 : 1ω7c. The polar lipids of strain KHS02T comprised phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminolipids, an unidentified phospholipid and an unidentified lipid. The genomic DNA G+C content was 58.6 mol%. DNA–DNA hybridization showed that the isolate shared 16.2 ± 1.3 % (reciprocal, 15.7 ± 2.8 %) genomic relatedness with the type strain of the closest species. In conclusion, this isolate is suggested to represent a novel species of the genus Pseudahrensia, for which the name Pseudahrensia todarodis is proposed. The type strain is KHS02T ( = KACC 18257T = JCM 30419T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000888