1887

Abstract

A Gram-stain-variable, short-rod-shaped, endospore-forming, strictly aerobic, non-motile, chitinolytic and endophytic bacterium, designated strain CC-Alfalfa-19, exhibiting unusual bipolar appendages was isolated from a root nodule of alfalfa ( L.) in Taiwan and subjected to a polyphasic taxonomic study. Based on 16S rRNA gene sequence analysis, strain CC-Alfalfa-19 was found to be most closely related to CAU 9324 (95.2 %), whereas other species of the genus shared ≤ 95.0 % sequence similarity. The phylogenetic analysis revealed a distinct phyletic lineage established by strain CC-Alfalfa-19 with respect to other species of the genus . Fatty acids comprised predominantly anteiso-C, C, anteiso-C and iso-C. Menaquinone 7 (MK-7) was identified as the sole respiratory quinone and the genomic DNA G+C content was 42.7 mol%. Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, an unidentified glycolipid and an unidentified lipid. The diagnostic diamino acid found in the cell-wall peptidoglycan was -diaminopimelic acid. Based on the polyphasic taxonomic evidence that was in line with the genus and additional distinguishing characteristics, strain CC-Alfalfa-19 is considered to represent a novel species, for which the name sp. nov. (type strain CC-Alfalfa-19 = BCRC 80441 = JCM 18446) is proposed.

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2015-11-01
2019-10-20
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