@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.000505, author = "Lai, Wei-An and Hameed, Asif and Lin, Shih-Yao and Hung, Mei-Hua and Hsu, Yi-Han and Liu, You-Cheng and Shahina, Mariyan and Shen, Fo-Ting and Young, Chiu-Chung", title = "Paenibacillus medicaginis sp. nov. a chitinolytic endophyte isolated from a root nodule of alfalfa (Medicago sativa L.)", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2015", volume = "65", number = "Pt_11", pages = "3853-3860", doi = "https://doi.org/10.1099/ijsem.0.000505", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000505", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A Gram-stain-variable, short-rod-shaped, endospore-forming, strictly aerobic, non-motile, chitinolytic and endophytic bacterium, designated strain CC-Alfalfa-19T, exhibiting unusual bipolar appendages was isolated from a root nodule of alfalfa (Medicago sativa L.) in Taiwan and subjected to a polyphasic taxonomic study. Based on 16S rRNA gene sequence analysis, strain CC-Alfalfa-19T was found to be most closely related to Paenibacillus puldeungensis CAU 9324T (95.2 %), whereas other species of the genus Paenibacillus shared ≤ 95.0 % sequence similarity. The phylogenetic analysis revealed a distinct phyletic lineage established by strain CC-Alfalfa-19T with respect to other species of the genus Paenibacillus. Fatty acids comprised predominantly anteiso-C15 : 0, C16 : 0, anteiso-C17 : 0 and iso-C16 : 0. Menaquinone 7 (MK-7) was identified as the sole respiratory quinone and the genomic DNA G+C content was 42.7 mol%. Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, an unidentified glycolipid and an unidentified lipid. The diagnostic diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid. Based on the polyphasic taxonomic evidence that was in line with the genus Paenibacillus and additional distinguishing characteristics, strain CC-Alfalfa-19T is considered to represent a novel species, for which the name Paenibacillus medicaginis sp. nov. (type strain CC-Alfalfa-19T = BCRC 80441T = JCM 18446T) is proposed.", }