1887

Abstract

A Gram-negative-staining, aerobic, non-motile, non-spore-forming, rod-shaped and yellow-pigmented bacterium, designated R11H, was isolated from a soil sample collected from a hexachlorocyclohexane dumpsite located at Ummari village, Lucknow, Uttar Pradesh, India. The 16S rRNA gene sequence similarity between strain R11H and the type strains of species of genus with validly published names ranged from 93.75 to 97.85 %. Strain R11H showed the highest 16S rRNA gene sequence similarity to DS15 (97.85 %), followed by JCM15910 (97.79 %), KCTC 12582 (97.77 %) and KCTC 22112 (97.34 %). The DNA G+C content of strain R11H was 63.5 mol%. DNA–DNA relatedness between strain R11H and its closest phylogenetic neighbours was well below the threshold value of 70 %, which suggested that strain R11H represents a novel species of the genus . The major polar lipids of strain R11H were sphingoglycolipid and other lipids commonly reported in this genus, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylmonomethylethanolamine. Spermidine was detected as the major polyamine. The chemotaxonomic markers in strain R11H confirmed its classification in the genus , i.e. Q-10 as the major ubiquinone and summed feature 8 (Cω7 and/or Cω6), summed feature 3 (Cω7 and/or Cω6), C and C 2-OH as the predominant fatty acids. Results obtained from DNA–DNA hybridization and chemotaxonomic and phenotypic analyses clearly distinguished strain R11H from its closest phylogenetic neighbours. Thus, strain R11H represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R11H ( = DSM 28472 = MCC 2778).

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2015-10-01
2020-09-28
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