1887

Abstract

A Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped bacterium, designated Q-4, was isolated from a herbicide-contaminated soil sample in Nanyang, Henan province, China. Strain Q-4 grew optimally in the LB medium without NaCl supplement at a pH range of 6.0–7.0 and a temperature of 30 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Q-4 was most closely related to ‘’ TDMA-5 (97.4 % 16S rRNA gene sequence similarity), followed by S27 (95.8 %). The genomic DNA G+C content of strain Q-4 was 41.8 mol%. MK-7 was the major respiratory quinone. Phosphatidylethanolamine and phosphoaminolipid were the major polar lipids. The major fatty acids were iso-C, iso-C 3-OH, Cω6 and/or Cω7 (summed feature 3) and Cω7/Cω6 (summed feature 3). Strain Q-4 showed low DNA–DNA relatedness with ‘’ TDMA-5 (21.4 ± 0.6 %). Physiological and biochemical characteristics are able to distinguish strain Q-4 from the most closely related species of the genus . On the basis of genotypic and phenotypic data, strain Q-4 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Q-4 ( = KCTC 42442 = ACCC 19798).

Funding
This study was supported by the:
  • , the National High Technology Research and Development Program of China , (Award 2012AA101403)
  • , the Project for Science and Technology of Jiangsu Province , (Award BE2012749)
  • , the Project for Science and Technology of Guangdong Province , (Award 2013B090500017)
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2015-10-01
2020-09-29
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