1887

Abstract

A novel Gram-stain-positive, spore-forming, aerobic, motile and rod-shaped bacterium designated strain PAMC 80007 was isolated from an active layer soil sample of Council, Alaska. Optimal growth of strain PAMC 80007 was observed at 30 °C, pH 7.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain PAMC 80007 belonged to the genus . This strain was closely related to (98.3 %), (98.3 %) and (97.2 %). Genomic DNA G+C content was 43.5 mol% and genomic relatedness analyses based on the average nucleotide identity and the genome-to-genome distance showed that strain PAMC 80007 is clearly distinguished from the closely related species of the genus . The major fatty acids (>5 %) were iso-C (24.7 %), Cω11 (16.8 %), anteiso-C (16.5 %), C (15.6 %) and anteiso-C (8.7 %). The major respiratory isoprenoid quinones were menaquinone-6 (MK-6) and menaquinone-7 (MK-7), and the polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, phospholipid and two unidentified lipids. -Diaminopimelic acid (type A1γ) was present in the cell-wall peptidoglycan, and the major whole-cell sugar was ribose with a minor quantity of glucose. Results from a polyphasic study suggested that strain PAMC 80007 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is PAMC 80007 ( = JCM 30371 = KCTC 33549 = DSM 29572). An emended description of the genus is also provided.

Funding
This study was supported by the:
  • (Award PE15080 and PN15082)
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2015-10-01
2021-10-16
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