1887

Abstract

Seventeen strains of lespedeza rhizobia, a previously defined novel group, were further characterized by comparison with phylogenetically related species using a polyphasic approach, including SDS-PAGE of whole-cell proteins, PCR-based restriction fragment length polymorphism analysis of ribosomal intergenic spacer sequences, determination of DNA G+C contents, DNA–DNA hybridization and analysis of phenotypic features. Based on data obtained in the present and previous studies, a novel species, sp. nov., is proposed for these strains. The type strain of is CCBAU 41251 (=LMG 24208=HAMBI 2971). Strain CCBAU 41251 could form ineffective nodules on in addition to its original host .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65661-0
2008-06-01
2022-01-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/6/1364.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65661-0&mimeType=html&fmt=ahah

References

  1. De Ley, J.(1970). Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol 101, 738–754. [Google Scholar]
  2. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  3. Gao, J. L., Sun, J. G., Li, Y., Wang, E. T. & Chen, W. X.(1994). Numerical taxonomy and DNA relatedness of tropical rhizobia isolated from Hainan Province, China. Int J Syst Bacteriol 44, 151–158.[CrossRef] [Google Scholar]
  4. García-Fraile, P., Rivas, R., Willems, A., Peix, A., Martens, M., Martínez-Molina, E., Mateos, P. F. & Velázquez, E.(2007).Rhizobium cellulosilyticum sp. nov., isolated from sawdust of Populus alba. Int J Syst Evol Microbiol 57, 844–848.[CrossRef] [Google Scholar]
  5. Gu, C. T., Wang, E. T., Sui, X. H., Chen, W. F. & Chen, W. X.(2007). Diversity and geographical distribution of rhizobia associated with Lespedeza spp. in temperate and subtropical regions of China. Arch Microbiol 188, 355–365.[CrossRef] [Google Scholar]
  6. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  7. Quan, Z.-X., Bae, H.-S., Baek, J.-H., Chen, W.-F., Im, W.-T. & Lee, S.- T.(2005).Rhizobium daejeonense sp. nov. isolated from a cyanide treatment bioreactor. Int J Syst Evol Microbiol 55, 2543–2549.[CrossRef] [Google Scholar]
  8. Rasolomampianina, R., Bailly, X., Fetiarison, R., Rabevohitra, R., Béna, G., Ramaroson, L., Raherimandimby, M., Moulin, L., De Lajudie, P. & other authors(2005). Nitrogen-fixing nodules from rose wood legume trees (Dalbergia spp.) endemic to Madagascar host seven different genera belonging to α- and β-Proteobacteria. Mol Ecol 14, 4135–4146.[CrossRef] [Google Scholar]
  9. Sneath, P. H. A. & Sokal, R. R.(1973).Numerical Taxonomy. The Principles and Practice of Numerical Classification. San Francisco: W. H. Freeman.
  10. Stackebrandt, E., Frederiksen, W., Garrity, G. M., Grimont, P. A. D., Kämpfer, P., Maiden, M. C. J., Nesme, X., Rosselló-Mora, R., Swings, J. & other authors(2002). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef] [Google Scholar]
  11. Tan, Z. Y., Xu, X. D., Wang, E. T., Gao, J. L., Martínez-Romero, E. & Chen, W. X.(1997). Phylogenetic and genetic relationships of Mesorhizobium tianshanense and related rhizobia. Int J Syst Bacteriol 47, 874–879.[CrossRef] [Google Scholar]
  12. Valverde, A., Igual, J. M., Peix, A., Cervantes, E. & Velázquez, E.(2006).Rhizobium lusitanum sp. nov., a bacterium that nodulates Phaseolus vulgaris. Int J Syst Evol Microbiol 56, 2631–2637.[CrossRef] [Google Scholar]
  13. Vincent, J. M.(1970).A Manual for the Practical Study of the Root-Nodule Bacteria. Oxford: Blackwell Scientific.
  14. Yao, Z. Y., Kan, F. L., Wang, E. T., Wei, G. H. & Chen, W. X.(2002). Characterization of rhizobia that nodulate legume species of the genus Lespedeza and description of Bradyrhizobium yuanmingense sp. nov. Int J Syst Evol Microbiol 52, 2219–2230.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65661-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65661-0
Loading

Data & Media loading...

Supplements

vol. , part 6, pp. 1364 - 1368

Phenotypic characteristics of strains of sp. nov. [ PDF] 186 KB



PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error