
Full text loading...
Seventeen strains of lespedeza rhizobia, a previously defined novel group, were further characterized by comparison with phylogenetically related species using a polyphasic approach, including SDS-PAGE of whole-cell proteins, PCR-based restriction fragment length polymorphism analysis of ribosomal intergenic spacer sequences, determination of DNA G+C contents, DNA–DNA hybridization and analysis of phenotypic features. Based on data obtained in the present and previous studies, a novel species, Rhizobium miluonense sp. nov., is proposed for these strains. The type strain of R. miluonense is CCBAU 41251T (=LMG 24208T=HAMBI 2971T). Strain CCBAU 41251T could form ineffective nodules on Phaseolus vulgaris in addition to its original host Lespedeza chinensis.
Article metrics loading...
Full text loading...
References
Data & Media loading...
Supplements
International Journal of Systematic and Evolutionary Microbiology vol. 58 , part 6, pp. 1364 - 1368
Supplementary Table S1. Phenotypic characteristics of strains of Rhizobium miluonense sp. nov. [ PDF] 186 KB