@article{mbs:/content/journal/ijsem/10.1099/ijs.0.65661-0, author = "Gu, Chun Tao and Wang, En Tao and Tian, Chang Fu and Han, Tian Xu and Chen, Wen Feng and Sui, Xin Hua and Chen, Wen Xin", title = "Rhizobium miluonense sp. nov., a symbiotic bacterium isolated from Lespedeza root nodules", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2008", volume = "58", number = "6", pages = "1364-1368", doi = "https://doi.org/10.1099/ijs.0.65661-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65661-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "RFLP, restriction fragment length polymorphism", keywords = "IGS, intergenic spacer", abstract = "Seventeen strains of lespedeza rhizobia, a previously defined novel group, were further characterized by comparison with phylogenetically related species using a polyphasic approach, including SDS-PAGE of whole-cell proteins, PCR-based restriction fragment length polymorphism analysis of ribosomal intergenic spacer sequences, determination of DNA G+C contents, DNA–DNA hybridization and analysis of phenotypic features. Based on data obtained in the present and previous studies, a novel species, Rhizobium miluonense sp. nov., is proposed for these strains. The type strain of R. miluonense is CCBAU 41251T (=LMG 24208T=HAMBI 2971T). Strain CCBAU 41251T could form ineffective nodules on Phaseolus vulgaris in addition to its original host Lespedeza chinensis.", }