1887

Abstract

A yellowish brown bacterium was isolated from photoheterotrophic enrichment cultures obtained from a mud sample collected from a mangrove forest located in Khola, India. Enrichment and isolation in medium containing 2 % NaCl (w/v) yielded strain JA297, cells of which were ovoid and motile. Cells of strain JA297 contained vesicular internal membranes and bacteriochlorophyll and carotenoids of the spheroidene series. Strain JA297 grew optimally at 30 °C and at pH 6.0–7.0. Photo-organoheterotrophy was the preferred mode of growth. Strain JA297 was also able to grow photolithoautotrophically and chemo-organotrophically but not by chemolithoautotrophy or by fermentation. Strain JA297 was able to utilize sulfide, sulfite, sulfate, thiosulfate, thioglycolate and cysteine as sulfur sources. Biotin, niacin and thiamine were required as growth factors. On the basis of 16S rRNA gene sequence analysis, strain JA297 was shown to belong to the class and was closely related to the type strains of (96.72 % similarity), (96.42 %) and (96.32 %). The DNA G+C composition was 63 mol%. Based on 16S rRNA gene sequence analysis and morphological and physiological characteristics, strain JA297 could be distinguished from all recognized species of the genus . This strain is therefore suggested to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JA297 (=ATCC BAA-1544=JCM 14888=CCUG 55397).

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2008-07-01
2019-10-17
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References

  1. Biebl, H. & Pfennig, N. ( 1981; ). Isolation of members of the family Rhodospirillaceae. In The Prokaryotes, pp. 167–273. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. G. Schlegel. New York: Springer.
  2. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Department of Genome Sciences, University of Washington, Seattle, USA.
  3. Hansen, T. A. & Veldkamp, H. ( 1973; ). Rhodopseudomonas sulfidophila, nov. spec., a new species of the purple non-sulfur bacteria. Arch Microbiol 92, 45–58.
    [Google Scholar]
  4. Heising, S., Dilling, W., Schnell, S. & Schink, B. ( 1996; ). Complete assimilation of cysteine by a newly isolated non-sulfur purple bacterium resembling Rhodovulum sulfidophilum (Rhodobacter sulfidophilus). Arch Microbiol 165, 397–401.[CrossRef]
    [Google Scholar]
  5. Hiraishi, A. & Ueda, Y. ( 1995; ). Isolation and characterization of Rhodovulum strictum sp. nov. and some other purple nonsulfur bacteria from colored blooms in tidal and seawater pools. Int J Syst Bacteriol 45, 319–326.[CrossRef]
    [Google Scholar]
  6. Imhoff, J. F. & Trüper, H. G. ( 1992; ). The genus Rhodospirillum and related genera. In The Prokaryotes, 2nd edn, pp. 2141–2155. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K.-H. Schleifer. New York: Springer.
  7. Kompantseva, E. I. ( 1985; ). Rhodobacter euryhalinus sp. nov., a new halophilic purple bacterial species. Mikrobiologiia 54, 974–982 (in Russian).
    [Google Scholar]
  8. Neutzling, O., Imhoff, J. F. & Trüper, H. G. ( 1984; ). Rhodopseudomonas adriatica sp. nov., a new species of the Rhodospirillaceae, dependent on reduced sulfur compounds. Arch Microbiol 137, 256–261.[CrossRef]
    [Google Scholar]
  9. Pfennig, N. & Trüper, H. G. ( 1992; ). The family Chromatiaceae. In The Prokaryotes. A Handbook on the Biology of Bacteria. Ecophysiology, Isolation, Identification, Applications, 2nd edn, pp. 3200–3221. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K.-H. Schleifer. New York: Springer.
  10. Srinivas, T. N. R., Anil Kumar, P., Sasikala, Ch., Ramana, Ch. V., Süling, J. & Imhoff, J. F. ( 2006; ). Rhodovulum marinum sp. nov., a new phototrophic purple non-sulfur alphaproteobacterium from marine tides of Visakhapatnam. Int J Syst Evol Microbiol 56, 1651–1656.[CrossRef]
    [Google Scholar]
  11. Srinivas, T. N. R., Anil Kumar, P., Sasikala, Ch. & Ramana, Ch. V. ( 2007a; ). Rhodovulum imhoffii sp. nov. Int J Syst Evol Microbiol 57, 228–232.[CrossRef]
    [Google Scholar]
  12. Srinivas, T. N. R., Anil Kumar, P., Sasikala, Ch., Ramana, Ch. & Imhoff, J. F. ( 2007b; ). Rhodovulum visakhapatnamense sp. nov. Int J Syst Evol Microbiol 57, 1762–1764.[CrossRef]
    [Google Scholar]
  13. Straub, K. L., Rainey, F. A. & Widdel, F. ( 1999; ). Rhodovulum iodosum sp. nov. and Rhodovulum robiginosum sp. nov., two new marine phototrophic ferrous-iron-oxidizing purple bacteria. Int J Syst Bacteriol 49, 729–735.[CrossRef]
    [Google Scholar]
  14. Thompson, J. D., Higgins, D. G., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
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